XB-CLONE-4357471
Clone Name: IMAGE:7029264
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Gene:
crx
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC085002 [+]
GCCTGTCCCAGGCTTGGACTACTGGTATAAGACACAGATGAGCACAAAGTACCATCGTATGCCTCTTCGATAGTTTAGTGGCGTGGATCTCAGACTTTGA
TGTATACTTTACAGATTCAGCTGAGAGAAGACCTGTATCTGGATCAACTGAGAGTAAAGAAACTCTGATTTCAGGCTAGCATACCACAAAAATATCAAGT
TTATTGAAGTCCCCTGAAACTCGGCCACTGAAATGATGTCCTACATTAAACAACCTCATTATGCAGTCAATGGACTTACTCTAGCTGGTACTGGAATGGA
CCTTCTGCACTCAGCTGTAGGATACCCAACAACCCCACGGAAACAAAGAAGAGAGAGAACAACTTTTACCAGGGCCCAGCTGGATATTTTGGAAGCCCTC
TTTGCTAAAACACGCTACCCTGACATTTTCATGAGGGAGGAGGTGGCTCTAAAGATAAATCTACCAGAGTCCCGAGTGCAGGTCTGGTTTAAAAATAGAA
GGGCAAAATGTCGCCAGCAACAACAGCAGAGCACCGGACAAGCTAAGCCTCGACCAGCTAAGAAGAAAACTTCCCCTGCCAGGGAGACCAATTCAGAGGC
AAGCACCAATGGGCAGTACAGTCCTCCTCCTCCTGGCACTGCAGTTACCCCTAGTTCCACAGCTAGTGCAACAGTGTCCATATGGAGTCCAGCCTCCATA
TCCCCCATTCCTGATCCTCTCTCTGCTGCGACTACTCCCTGCATGCAGAGGTCAGCTGGCTATCCAATGACCTATAGTCAGGCCCCTGCTTATACTCAGA
GCTATGGAGGATCTTCATCTTATTTCACAGGGCTGGACTGTGGATCCTATCTATCTCCTATGCACCCACAGCTCTCAGCTCCTGGTGCCACATTGAGCCC
AATTGCCACGCCTACGATGGGTAGCCACCTTAGCCAATCTCCGGCATCCCTTTCCGCCCAGGGATATGGAGCTTCCAGTCTTGGCTTCACCTCAGTGGAT
TGCTTAGACTACAAAGACCAAACTGCTTCCTGGAAGCTAAACTTCAATGCCACTGACTGCCTTGATTATAAAGACCAGAGCTCATGGAAATTTCAGGTCT
TGTAGATATGACTGAAGTCCAAGCCTCATTTTCATCATCCTCAACTTCAGTATAAACTGTGCTTCATATTTCAGCTTTACAAGAGTGGCTTGACTGGATA
TCTAACGATGGAACAAACACAAAAACATTAATAACTTTATATCTTTATGCCATTAACTCTTTTAGAACAAATTGCCTGTACAACTCCACAGTTTGAAACC
ACACAAATGTCTGTAAAAACACAGCACACCAAGCTTATTTTCTTTTTTAATCTGAAAAACATAAAGTTTTGATAAAATGACAACAAATGTAGGGGAGATC
ATTTTAAAAGCACTTTGGCCTGAGGAAGCTATGATAACAGTTCCTTACTCCCCTATCTTGTTGTTGATTGGGAAATTTGTTGTGATAAAATAATCAGGTT
TTTGGCATTAAACCTGTTTTGCTGCACATTTTGCTTACTCTGCCCTAGCCACCAAAGATAATTCAGTAATCTCTGGAGTAAAAGTGTTCTCCAGATATCT
ACCAAAGTTTCAAAATGAGATGTGACTCAACACAGCCATTCCTTATCTTTCCAATTTCTTAACCCTTATAATGCATATTTGAACTTTAATTTAAATTGAT
ATCTGATAACGCACAGAAGTTCATCAGGTGCATATGAGCCACCTACTGCAAAACCTGTATTTAAAGCCCCCCTACCAACAGTGAAAAAGAATGAAAAGTA
ATCTGCTCTGTTCAGTTTAGGACACAATGCCTGAAAGGCACAACCTTCTGCAGATTCTGAAATCCAATCAAGCTAAACAATAGCAGGCTCCTCATGTACC
AGAGCTAACACAATCACTGTAGTTCATTACAAGCATTGTTCATTTATTTGAACAAGCATAAACTTGAGACTCACAACTAACCAATGATATTTTTGAATGG
AGTTAATTTATTTTATAAAAGGTTTCTACCTATCCACCGCTGTATGATTTGTCATACCATGGAATACTATAAATGAGAAGTGTTTTTCTGTGTTGTGCTT
TAGTCTTTGTGTTAACTAGCAAAGTTCTATATAGGGGTTTATGTCTGTCCCAGCAAATCAGTACAAACTTTCTTTTAGTTCTGTAATATTAATTAATTAA
TGTTATGACAAAAAATATAAATGTGCAAAATATTAAAATTATTGTGTTAAACCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF781449 [+]cccccccccccccgcctgtcccaggcttggactactggtataagacacagatgagcacaaagtaccatcgtatgcctcttcgatagtttagtggcgtgga
tctcagactttgatgtatactttacagattcagctgagagaagacctgtatctggatcaactgagagtaaagaaactctgatttcaggctagcataccac
aaaaatatcaagtttattgaagtcccctgaaactcggccactgaaatgatgtcctacattaaacaacctcattatgcagtcaatggacttactctagctg
gtactggaatggaccttctgcactcagctgtaggatacccaacaaccccacggaaacaaagaagagagagaacaacttttaccagggcccagctggatat
tttggaagccctctttgctaaaacacgctaccctgacattttcatgagggaggaggtggctctaaagataaatctaccagagtcccgagtgcaggtctgg
tttaaaaatagaagggcaaaatgtcgccagcaacaacagcagagcaccggacaagctaagcctcgaccagctaagaagaaaacttcccctgccagggaga
ccaattcagaggcaagcaccaatggggcagtacaggtcctcctcctcctggcactgcagttacccctagtttccacagctagtgcaacagtgtccatatg
ggagtccagccctccctatccccccattcctgatccttctctctggctgcggactactcccggggcatgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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