XB-CLONE-4357395
Clone Name: IMAGE:7029205
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Gene:
mal
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077032
[+]
CCCGACCAGAAACAGCAGGATTTGGGAGGAAGATCACAACCTGTGCTTTTCCTCAGCCTTGAGGGAAGAAGAGGGAATAGACCAATATATCTAAATCACC
AGATCTGCAGTAATTGACATGGCAGCCACAACTCCTAATGCCCCAGTATATGAAGATACATCTTCTTTGCCATCTGGTTTCAGAGTTTTGGCAACATTTC
CTGATGTGCTGATGATACCAGATTTAGTTTTTGGAAGTCTTGTATGGATTTTGGTGGCTTCTTCAAGAGTGCCATTTCCCATTGTACAAGGATGGGTTAT
GTATGTCTCCGTATCCCTTTTTTTCTTCAGTGTGCTCCTCATGCTGCTGTATTGCGCTCGTGCACATGGAGGAAAGTCATCCTGGACAGCAGTTGATGCT
TTTTATCACTGCGTTGCTGCTTACTTCTACTTGAGTGCCTCTGTCCTAGAAAGCTACAGCACCATTGCACTGAGTAATGTGACCATATATCAGCTTTATC
AAGAGAATATTGCAGCTGTGGTGTTTGCCTATCTTGCCACACTTTCCTATACAATTCATGCTATTTTCTCACTGATACGGTGGAAAGGATCATCCTAATA
CTGAAACATGTGGTAACGATTCGGAGTCGCACAATCTACTTGAAATAAAAGCTAAACTACAATATGGACTGGCAGAGACACTAGCAAAATACTTGTCCCA
TGTGTACGTACTATGTTAGGAATATATTCGGATTTAGCATCAGATTGCACAGATAGTTACACGCCCGAGCCCCTGCGCCGATCAAAGTGAACACATCACT
AAAGATAAATCTACCAATGTCTTCCCTTCAGTTACAAATGTTTGTGTTTTTATTTGTGTTTTATAGGTATTTTTTCCTGTAACAGTTTGAGCAAATGTAT
TTAATGTTAATGTGTGTTTACAGTTTACAGAATGTGATTTAATCTCTTATTTGAATTGTAAATATGCAGCCAAAAATTAGTCACATACAAATCAATTTTA
GAGAACAAATAATAAAATAAATGTAATTTTCACTTAAAGAAAAAAAAAAAAAAAA
5' EST: CF781487
[+]taattaatccccccccccccccccgaccagaaacagcaggatttgggaggaagatcacaacctgtgcttttcctcagccttgagggaagaagagggaata
gaccaatatatctaaatcaccagatctgcagtaattgacatggcagccacaactcctaatgccccagtatatgaagatacatcttctttgccatctggtt
tcagagttttggcaacatttcctgatgtgctgatgataccagatttagtttttggaagtcttgtatggattttggtggcttcttcaagagtgccatttcc
cattgtacaaggatgggttatgtatgtctccgtatccctttttttcttcagtgtgctcctcatgctgctgtattgcgctcgtgcacatggaggaaagtca
tcctggacagcagttgatgctttttatcactgcgttgctgcttacttctacttgagtgcctctgtcctagaaagctacagcaccattgcactgagtaatg
tgaccatatatcagctttatcaagagaatattgcagctgtggtgtttgcctatcttgccacactttcctatacaattcatgctattttctcactgatacg
ggtggaaaggatcatcctaatactgaaacatgtggtaacgattcggagtcgcacaatctacttgaaataaaagctaaactacaatatggactggcagaga
cactagcaaaatacttgtcccatgtgtacgtactatgtttaggattatattccggatttagcatcagattgcacagatagttacacgcccgagccccctg
cggccgatcaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: