XB-CLONE-4357307
Clone Name: IMAGE:7029113
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Gene:
map1lc3a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080488
[+]
CGAGAGAGAGACTGGGCGAGTCGGGCTGAGTGCTAGCTTCTGGGACTCACACCCCTCAGGATGCCGTCAGAGCGGCCCTTTAAGCACAGGAGAACCTTCG
CCGAGCGTTGTGCAGAAGTACGGCAGATCCGTGAGCAGCATCCCAATAAAATTCCGGTTATTATAGAGCGATACAAGGGGGAGAAGCAGCTGCCTGTGTT
GGATAAAACAAAATTCCTTGTTCCAGATCATGTGAATATGAGTGAACTAGTGAAAATCATAAGACGCAGGCTGCAATTGAACCCCACCCAGGCCTTCTTC
CTGCTTGTTAACCAGCACAGCATGGTGAGTGTCTCCACCCCAATACTGGATATCTACGAACAGGAGAAGGATGAGGACGGCTTCCTGTACATGGTGTATG
CCTCGCAGGAAACATTCGGCCACTGATGTCCTGAGGATGCACAATCGCTCGCACGCCAACACACATCCAACACGGCTATGCACAGACACACTCTTGCATA
TTGACACTTACATGTGATTTTAACCACACTTCTTGCCTGTAGATACCAACAAATCATTCTTGCACAAGTACTGCCTCGCACAAATACTGTTACACGAGCA
TTCCACCATGCTTTGGACCCTTGTTAATGCAATGTGTGTATGCGCACACTTATTTCTGTGCACACATTCATACCCCCCATGCCTGTATGTAAGATTTCAT
GCTTGCACGCAATCAACCTTCCGCTGCCATGCAGAATCTCCTGGTTACACACACGCAGTAAAAATGGGGTGCCCCATGTGTAACACACTTTCCTGCAACA
AAGGTTTGCCCTTTTGTGTAACTGTAGATAGAGTTTTTGTGGCTCATATTTGTGAGGTTGCAAACAGGGGCACAAATTATAGAAATGTGGACATTTAGGG
GCCCGAGGACCACTGAAAGTACAAAGGGTATTAATATCGGCGTTCCATCACAAAATGGAGTCCATAATTTTATAATTTGGGTCTATATTGTCTGGTCTCA
TCAGTGGAGCTTAGGGAGTAAAGGGGACAATAAACCTTTGAGGTTAACCCTTTGTTCAAAAAACTGCAGCACTCACAGATATCCACTACAGTTTCCTGCT
TTATGGTTTATCAGGATTTCCAGTGTGTAATCATGGAAGCACTTTAAGAGCTCCTTCTGTTGTCCAGAGGAATTCACTGCGGTCACAAGTAATAACAACA
TGGCAGCTTTTTAGTATAACATCAAACCAGTGCTGGCCAATGGGCTTACTCACGTGGTTTACTGTTTCTTTTTTAGGGGGGCAAAATATATACCTGCTGT
TAGGCCCACTGTTATTATGCCTCCGCTTGCACTTCTTGTAGCCGCTGGTCGTTGGCCATAGCAACAACACCATTAACTTTCGCTATTTGTAGATTTTAGT
AAACAGCAAGATACAAAAGAGAAAAAAAAAATCAGTTCTCTCGAAAATGGAGATACTTGTGTGTAACCGATTCTTTCTACCTGTACGATTTATTTTATTC
TTTTAATCTGCCAAATTCTGGCATAAATCTGATTTTCTTGAGTGTTATAGTGATTGATAATCATGAATACGGAGTGACTAGGGGGGGTCGGGGGGGGGGT
TGTTAATAGCTGTACAGAGTTAGACTTCAGTTTTCTGTCTCGCTAAAGTCTCCTTTTTCTGTTTTTAGTTATTTTAATCTTTTTGTTGTTCTCCTGATGG
AACGTAAAACGGTGAGAGGGTAAATGAGACTTCCCCTCTCTATACTGCAATGAGAAAATGTACAAAAAAAAACTATATAAATATAAAAAGCTGATGTGTG
TATATACTGATACATTTCATGTGTATAAATAAGAATAAAAAAAATATTGAAAGAAAAAAAAAAAAAAAA
5' EST: CF781531
[+]taattaatccccccccccccccgagagagagactgggcgagtcgggctgagtgctagcttctgggactcacacccctcaggatgccgtcagagcggccct
ttaagcacaggagaaccttcgccgagcgttgtgcagaagtacggcagatccgtgagcagcatcccaataaaattccggttattatagagcgatacaaggg
ggagaagcagctgcctgtgttggataaaacaaaattccttgttccagatcatgtgaatatgagtgaactagtgaaaatcataagacgcaggctgcaattg
aaccccacccaggccttcttcctgcttgttaaccagcacagcatggtgagtgtctccaccccaatactggatatctacgaacaggagaaggatgaggacg
gcttcctgtacatggtgtatgcctcgcaggaaacattcggccactgatgtcctgaggatgcacaatcgctcgcacgccaacacacatccaacacggctat
gcacagacacactcttgcatattgacacttacatgtgattttaaccacacttcttgcctgtagataccaacaaatcattcttgcacaagtactgcctcgc
acaaatactgttacacgagcattccaccatgctttggacccttgttaatgcaatgtgtgtatgcgcacacttatttctgtgcacacatttcttacccccc
atgcctgtatggaagattttctgcttgcacgcaatcaaccttccgctgccaggcaaaacccccgggttacccccccgccgtaaaaagggggggccccctt
gggtaaccacttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: