XB-CLONE-4357195
Clone Name: IMAGE:7029809
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Gene:
kpna2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081368 [+]
CGGCAGTCTGTTGGGATCCGCTGGCAAAGCGAGAGCTGTTGTGACGTGTTCTGTCTGTAACTTTATCCTCTGAAAATGTCCAACAATGAGAACGCTGCCC
GCCTCACCAGGTTCAAGAACAAGGGCAAGGACACCACGGAAATGAGGAGGAGGAGGATCGAAGTGAACGTGGAGCTGCGCAAGGCCAAGAAAGATGATCA
AATGCTAAAGAGGAGAAATGTCAGCACCTTCCCAGATGAGCCAACCTCTCCCCTACAGGAGAAGAACCAGAATGGGCAGACCTCTACACAGTGGAGTGTT
GAGGAGATTGTCCGGGGTGTCGCCAGCTCCAGCCTAGAAATACAGCTACAGGCCACACAGGCTGCGAGGAAACTTTTGTCCAGAGAGCGAGAGCCGCCAA
TCGATCGCATCATTCAGGCGGGTCTCATTCCCAAGCTGGTAGCGTTTCTGGCCCGCACCGACTGCAGCCCCATCCAGTTTGAGGCCGCCTGGGCTTTGAC
GAACATTGCTTCCGGAACCTCTGATCAAACCAAGGCTGTTGTGGAAGGCGGTGGTGTCCCGGCATTCATCTCCCTTTTGGCATCTCCTCATCCTCACATC
AGTGAGCAGGCTGTGTGGGCCTTGGGAAACATTGCAGGTGATGGATCTGCCTACCGTGATCTGGTTATTAAACATGGAGCTGTTGGGCCCCTGCTGGCCT
TACTGGCTGATCCTGACCTCTCCACATTAGCTACTGGTTACCTCAGAAATGTTACATGGACACTCTCTAACTTGTGCCGCAACAAGAACCCGGCCCCGCC
TCTGGATGCTATTCAGCAGATCTTGCCCACACTAGTGAGGCTATTGCATCATGATGACCGGGAGGTACTGGCTGATACCTGCTGGGCAGTGTCCTACCTC
ACCGATGGCTCAAATGACCGCATCAACGTGGTGGTCGGCACAGGCTTGGTCCCACGCATTGTCCAGCTGCTGGGCTGCGGAGAGCTGACAATTGTGACCC
CTTGTTTGAGAACTGTAGGGAACATCGTGACTGGCACGGATGAGCAGACCCAAGTGGTTCTTGATGCGGGGGCCCTGTCTGTCTTTGCCGAGCTGCTCAC
CCACCACAAGAATAACATCCAGAAGGAAGCTGCCTGGACATTGTCCAACATTACTGCTGGGCGTCAGGACCAGATCCAGGAAGTGGTCAATCATGGGCTG
ATTCCCTATCTCATTGAGATCCTGAGGAAGGCTGACTACGAGACCCAGAAAGAAGCTATTTGGGCAGTCACCAACTACACAAGTGGGGGAACAATCGATC
AGATTATCTACCTTGTCCAGTGCAGTGTCATTGAGCCTCTGTCAAATCTCCTCTCTGTTAAGGACAGCAAGACTGTTCTGGTCATTCTAGATGCCTTCAC
CAATGTGTTTGCTGCTGCTGAAAAGCTTGGGGAGACTGAGAAGCTGTGCCTCATGGTAGAGGAATGTGGTGGTCTCGATCGCATCGAGGCCCTGCAGTCT
CATGAGAATGAACAGGTCTACAAAGCATCAGCCGGTCTCATTGAGAAATACTTCTCTGCAGAGGACGGAGAAGATGAGTCTTTGGCTCCTGAGACCACAT
CCGACGGCTACACTTTCCAGGTGGAAGGTGGAACCCACACCACATTTAACTTCTAAACATTGCCCTGTCTGTCTGTCCTTTTCTATGTCAACTGGCACTT
TGTCTTATTTTGAACAGCCTTTTCCACTCTGTAAATATACTGTGAATCTTAAATCTGTATTGAAATAAAATGTTGTAAATATTTCCTTTTTAATTTACAG
CAGTCAGGCAGCAGCTGCAATGCTTTCTAGTTCAAAGGCAAATGTAGCTGTGGCCGCCATAACCTCTTGAGGCAGAGATTTTATTGTACGAAGCAATTGG
GCCAGCGGCTGGCACAGTCCGACCATAGAAGCACTTAGCACAATTAGACATAAGGGGGTTTTGTTGGTTCCCCCTGTGCCTTGTATGAACCATTAATACA
ATGGCCTGGTTCCCTGCCAGCCCTCACTGACTGCATACTGGCCACAGCTTAGGGATTGTTCTGGCCCACTAACTGGCCCATTAAGGAGAAACTGCCAGAT
ACTTGTGGATCTGTGTGGCCAGTGCTGGGGATGGCTGGCACAAGCCATGAAAGCATTTCTGTGGGGCTGTGTGTTCCATGTCTCTCGCTGTCTCTGCTCT
TGAGCCCCCTGTATCCCTGAGAAAGCGTTCTAGCTCGGGGGGCTGCACATTGAATTCTTTAAGTTCTTGTACAGCTTCTTTATATTGCCACTGTTTACAA
TAAATTCCGTTTTGGCTAAAAAAAAAAAAAAAA
5' EST: CF781587 [+]taattaatccccccccccccccggcagtctgttgggatccgctggcaaagcgagagctgttgtgacgtgttctgtctgtaactttatcctctgaaaatgt
ccaacaatgagaacgctgcccgcctcaccaggttcaagaacaagggcaaggacaccacggaaatgaggaggaggaggatcgaagtgaacgtggagctgcg
caaggccaagaaagatgatcaaatgctaaagaggagaaatgtcagcaccttcccagatgagccaacctctcccctacaggagaagaaccagaatgggcag
acctctacacagtggagtgttgaggagattgtccggggtgtcgccagctccagcctagaaatacagctacaggccacacaggctgcgaggaaacttttgt
ccagagagcgagagccgccaatcgatcgcatcattcaggcgggtctcattcccaagctggtagcgtttctggcccgcaccgactgcagccccatccagtt
tgaggccgcctgggctttgacgaacattgcttccggaacctctgatcaaaccaaggctgttgtggaaggcggtggtgtcccggcattcatctcccttttg
gcatctcctcatcctcacatcagtgagcaggctgtgtgggccttgggaaacattgcaggtgatggatctgcctaccgtgatctggttattaaacatggag
ctgttgggcccctgctggccttactggctgatcctgacctctccacattacctactggttaccctcanaaatgttancatggnacacctctctaaacttg
t
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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