XB-CLONE-4357185
Clone Name: IMAGE:7029804
|
Gene:
fgf3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC081367 [+]
GTCTTTAATGGGACCCCTTAAGTCATTTGTTTCCAGACTTCATCTACTTAAGGGACTTTGTGTTTCGGCCATGCCACAATGGTTATAATTTGGATTCTGT
TGCTGAGTTTTATCAGTTGTGGTCCGCAGGTATCCGGGGCGAAGAGGCTGGAGAAAGAAGCCAAGTCTCCTTGCTCCCGAGGCAAAGTGTGCGACCCCAG
GCAGAGACGAGATGCCGGGGGACGCGGAGGGGTTTACGAACATCTCGGAGGCGCCCCTCGGAACAGAAAACTTTACTGCGCTACTAAGTACCACCTACAG
ATACACCTCAACGGCAAGATTAATGGGACTCTGGAGAAAAACAGTGTATTTAGTATTTTAGAAATTACCGCCGTGGATGTGGGAATTGTTGCCATCAAAG
GGCTGTTTTCTGGGAGATATCTGGCCATGAACCAAAGAGGGAGACTTTACGCCTCGGAAACCTACAACCCCGAATGTGAGTTTGTGGAAAGAATTCACGA
GCTCGGATACAATACGTACGCGTCGCGCCTTTATCGGACGGTTCCGAGCGGGGCGGGCACCAAGCGCAAGGCCAGCGCAGAAAGACTGTGGTACGTGTCT
ATCAATGGCAAAGGGCGACCCAGAAGAGGCTTCAAGACAAGAAGGACGCAGAAGTCTTCCCTTTTTTTACCCAGGGTGCTAGACAACAAGGACCATGACG
CCGTGAGGTTATTTCACACGAACGCTGTGTATAGAGAAAGTATCCTGAAACCCTCCAAACCCAGCGGAAGGCAAAGGCGGGGACAATGAGGGCTCCAAAC
TGTGACGAGATATTCCCCTTCCGACGCCGCGTCTAAGAACGGGAAATGACGTTGTTCCCTTAGGACAAAAGACGATGATTCCATTTTGTAGAACTTTATT
TTTATGAGGCTGACAATACTGCCACTAGAACCCATATTCTGCCTCTCTGGGTCTCCGAATCCTCGGTGTTCTACTGTAAGACCCTCTGGTAGGGATTTCT
CATGTTTCCACCGCTGGTCCCAAATGTGCATCTTCGGCACTTTCCTTGGAACCGAAATCCGAGGGGCACGGAGACGTATCGGGTCGTTCAATTATTGGGT
CGTTCAATCCGTCCCCCCCACGTTTCTTTGTTATTTGATCTACAGCTCATCACACGTCGGCTTGACTCCGCCTTTGGGTCCTACCTCAGATTCTGATATA
AGATTCTGCCCTAAGTTATGCAAAAGAACAAATAATGGCAGAATGTAAATGTGCCTGCTGTAGGGGCAAGTTCTTCAAAACCTATTTATTACCATTCATG
AAGTACTGTATGGCCATTGGGAATGTGTGTGTGCATACATTGTCTTTTATCTTTCAATTATATAACATTTGGATGCAATGAGAAGCCTCTGCAGAAGATG
GTTGCTTAGCTCTCAGACTGTGAGTGGTGCCCACATAACCGTGTATCATCCAACGTTCTACTCTAGTCGAATATACTGTACGCGGTGCCGCTGGGAGCAC
TTTACTCGTAATATCGTTTGAATGCATTCCAGACATGTATCCTGGACATATCTTCTTAGCAACTTGGCATTCGGCTCATTGGCTGCGGTAGCATCGGCGA
TATGACTATGATTTGAAAGGGTTTTGCTGGTAGAAACATCCCAAAACCTAAGTCTGACCTTGAAAGTATTACTATAGCTGTTATCCTTGTTTTCCTGCCC
TGGGAACAACAGTACTAAAATAAATAATACATTTTATTAAAAAAAAAAAAAAAA
5' EST: CF781592 [+]taattaatcccccccccccccgtctttaatgggaccccttaagtcatttgtttccagacttcatctacttaagggactttgtgtttcggccatgccacaa
tggttataatttggattctgttgctgagttttatcagttgtggtccgcaggtatccggggcgaagaggctggagaaagaagccaagtctccttgctcccg
aggcaaagtgtgcgaccccaggcagagacgagatgccgggggacgcggaggggtttacgaacatctcggaggcgcccctcggaacagaaaactttactgc
gctactaagtaccacctacagatacacctcaacggcaagattaatgggactctggagaaaaacagtgtatttagtattttagaaattaccgccgtggatg
tgggaattgttgccatcaaagggctgttttctgggagatatctggccatgaaccaaagagggagactttacgcctcggaaacctacaaccccgaatgtga
gtttgtggaaagaattcacgagctcggatacaatacgtacgcgtcgcgcctttatcggacggttccgaagcggggcgggcaccaagcgcaaggccagcgc
agaaagactgtggtacgtgtctatcaatggcaaagggcgacccaaaagaagctttcagacnagaagggacgcaaaagtcttcccctttttttacccaggg
tggctagacaacaaggaccatggacgccgcttgaagttatttcacaccgaacgctggggtataagagaaaggtatccctgaaaaccctccaaaacccagc
gggaaagcaaaagccgggggaaaat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: