XB-CLONE-4357173
Clone Name: IMAGE:7029797
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Gene:
frg1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077685 [+]
GGTCCGGAAGCGCGTACTGTTTACAACTAGTTTAGCAACATCTCCTGTTAGGGTTTAATAGTGAGACAGGATCGTGTTGGGCTTCCACTGTAACTACAGA
AAGTATAACCTCCAGGATTACTCTGTCTGACAATGGCTGAATATTCAAAAGTGAAATCTACCAAGCTAGTGCTGAAGGGAATGAAAAACAAGAGTAAGAA
AAAGAACAAAGACAAAAAAAGGAAAAGAGAAGAATCTGATGAGGACAAGATGGATGTAGTTGGAAATTGGTGGACTGTTAAGAACTTTGGAGAAATTTCT
GGGACAGTGGCTATTGAAATGGACAAAGGAGCTTATATTCATGCTTTAGATACTGGCCTGTTTACTATTGGAGCTCCCCATAAAGATGATGATGGACCAT
CGCCTCCAGAGCAATTCACAGCAATCAAGTTGTCCGATTCAAGAATTGCACTAAAATCTGGCTATGGCAAATATCTTGGTATCAATTCTGATGGCCTTGT
CATTGGGCGTTCTGATGCCATAGGAGCAAGAGAGCAATGGGAACCAGTCTTTGAAAATGGGAAAATGGCACTTCTTGCTTCAAACAGTTGTTTTGTTGGA
TGTAATGAAGAAGGTGACTTAATAGCACAAAGTAAAACAGCTGGTGAAGGAGAAATGATAAAGATCCGCTCATGTGTTGAGAGAGATGGAAAAAGTGAAG
ATGAAATTCCCAAAGAAGATAAAGGAAATGTTAAGCAATGTGAAATAAACTATGTAAAGAAATTCCAGAGTTTCCAGGACAACAAACTCAAAGTGAGTCT
GGAAGACAACAAAGATCTAAAAAAGGCCAGAAAAGAAGGAAACTTTCACGAGACACTTCTAGACCGGAGATCTAAACTGAAAGCAGATCGATACTGCAAA
TGAAACATTGTGCTTGACCTGCTGTGTGAATATTTCTTTCTGTTCTATAAGTATTTATTGCTATAGGTTTTGTACTATTACATCATTAAAACGATTTTTT
TAACAACAAAAAAAAAAAAAAAAA
5' EST: CF781598 [+]taattaatcccccccccccccggtccggaagcgcgtactgtttacaactagtttagcaacatctcctgttagggtttaatagtgagacaggatcgtgttg
ggcttccactgtaactacagaaagtataacctccaggattactctgtctgacaatggctgaatattcaaaagtgaaatctaccaagctagtgctgaaggg
aatgaaaaacaagagtaagaaaaagaacaaagacaaaaaaaggaaaagagaagaatctgatgaggacaagatggatgtagttggaaattggtggactgtt
aagaactttggagaaatttctgggacagtggctattgaaatggacaaaggagcttatattcatgctttagatactggcctgtttactattggagctcccc
ataaagatgatgatggaccatcgcctccagagcaattcacagcaatcaagttgtccgattcaagaattgcactaaaatctggctatggcaaatatcttgg
tatcaattctgatggccttgtcattgggcgttctgatgccataggagcaagagagcaatgggaaccagtctttgaaaatgggaaaatggcacttcttgct
tcaaacagttgttttgttggatgtaatgaagaaagtgacttaatagcacaaagtaaaacagctgggtgaagagaaatgataaagatccgctcatgtgttg
agagagatggaanaagtgaagatgaaattcccaaagaagataaaggaaatgtttnnagcatgtgaaaataan
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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