XB-CLONE-4357151
Clone Name: IMAGE:7029784
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Gene:
dleu7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080492
[+]
GGCAGTTAGTGAGGGTGCGATTGTGTCGGAGACATGATGCAGAGACCCGGAATGGTCGGAGTCGCGCTAAACCACCAGGTCACAGCGTTCCACACGCTCA
GGGCTTTGCTGCAGGACAGGGGCTCAGAACCCTGTGTTCTCATAGAGGAACCGCACCCCCTGCCCATTACCGGCCTCACTGCACTGAATGATGAATGTGC
CGGGGAAGGTACCAGCACCGGTCGCACCAGGACTGCCAGGGAACTGCAGCCAGACTCTGCCAGCTTATACTCTGCCTCCGTCACTACATCCAGCGCACTA
AGGGCAGGATCTGCAGCGGGGACTAAAGCCCCTACACTGGCACAGATGGCATCGCAGAACCGCCTTTCCAGGGTCACCAACTCCACCGCTCAGCTCTTAC
TGGTGGAACAGAACATGCTGCAGCACCTACCGCCGGAGCGGCCCCTTGGCATTCAGCTGAAGGACAGCATTGAATTCAGAAACATCTGCACGCATATGGC
TCTTCAGGAGGAGGACCGCAAGTTTGATAAGGATGTGAATGAGGCCCAGCAATGCTTAAGGACAATCATCACCAATCTGATCACTGCCCTTTTAGCCCTG
AACTCAGAGTTTTGTGCCGCTGCCGCCGAGGATCTGCGCTCCATACTGAAAAAGCTCACTGAGGACTGAACAAAGATAGGACTGGTATGGAACAATAGGC
AATATGAATGGAAAGCCAGGAGTTGCCATAACCTATACAGTTATTGCACACAAGGAACAATTCATAAAGACTGGCACGTTCCATTGCATGAGCAGCACAG
GGACAAGATGGCTTCTCTGGTGGCTTCTCTTCTCTGACATCTGACAAACTGGCAGACATTCGTTTATCCTAAATAACACAATAATATGACATGTGAATAT
TTATGCTGAGTAAATGGTAGGGTGAATTTTAGTCAGTCTGGGATGCCTGCAGTTCAGCAGCCACTGGGAGTTGCAGTTCAGCAGCCACTGTAGGGTAAAC
AGTAATCTAATCTAGAGGCCTGAGCATGGGCTTCAGAGGTCCTGAGTATAAAAAGGAATGTTTTCTTTTATTTGCAGTCAGTATTATTTAGTGTATTTAT
TTATCTCTTTATTATGTGAAATCATGTTATGTGAAAACTCAGACTGGTCTCCTTCTGACCAATTCCCCCTAGACTGACAGTGTTATTCTATTCCTGACTC
TCATTCATCCATGCTGGAACGAAGCGCCTCTGCTATCCAGAGAATCTGTGCAGCACCCACCATGTCTCCTCCTGCCTACTCTTCTCTCTGCAGGTTGGGA
CCATGCCTACTGCCTGCCTAACCCTGCCTCCCATTGCCCCTTTCCCATAGGAAGAATCACTGCCCACTGCCCCCCATTCCTTTTTTGCACAGTTTTCGAT
GCTGCTATTTCACATTTGAACTATAACAAAGTATCGCCTGGCCAGTGGTGTAACTATGTATACAGCGGGGAAGTGCAGTGCCAGAGGGCAAGGGGGGGGG
GGGGAGGAGGGACTGGTTTTGCCGGGCCCCCTCAGAAGATTCACAGGGGGGCCCAGAGTGACCAAGTTACGTCGCTGCCCCTGGCATTGGGAAACACATT
GTGCAACAGGTTCTAGAACAAGCAGTGTGTTACTGCTGTGTAGCATTTTTTCAACTCTTTTATGAACATTACATAAGTATTCATTTGACAATTTGTTGTT
TCCTGTATTTGCCTTTATGTCACACACTTCTGTAGGTCCAGCATTCAGGGCATATTGAAGTGTGATGGACGTATGTAAGGTTCAAAACTGAACTTCTGTT
TCCATCATCAGTGCAATAGTAACCTCAGGTGTTCATTAATGTAACTGACAACCTTCTTGCCCAACCAATTTCCTCTGCACCCTCATGGCTACAGATCCAA
AAGGCTTATGGCACATCTGGCCATATATTGTAGCTTGTCAACTTGCATTTGTTTTTAGACTCCATTTTATTCCATTAATGTAAATGACAAGCTAAACAAT
TTGGGGAAAGTATGCCTGGCGCTGGGCCCAAACTAGGGGTAGGCAGAAGAGGCAAGTGCCTATGGCACAATGATGGGGAGGGGCGGGCACAAACCTCAAG
TGTCTGCTTTGCTAACTACCCCTCTTACAGGGGCCCTTGTCGCCCCACTGCACACTTAAGTCCTATTCTGTGTTGTCACCATGCACGCTTTCCCCTGTAC
GGGGGGCTGGTTAAGGCACGGTAACCAGCCGGGTTGCCTAGGGAACATTGGCAGGCTCTTTCTTGGCACCTCATGGGCTAAATTAACTTTTTACATGTGC
TATAGTGCAGTTGAGTCCGGTGAATGCATCCTATAACAACTGCAGTAGCATACACTGTGGGTACATTCTCTGTTTCTCATAATTCTGAAATGTGGAGATG
CCAGAATAAATGTTAATACTATAATGCTTGTGACTATATGTTTACAATGCGTTGTTTCCCATTGTGCTCACATATGGGCCCCAGCACATTTGGACCCCTC
TTGGATACTTTGGATAACTGTTCCTTAGGGCCCAGTGAGAGACAATCGCCTGTGCAGTCACACAGAAATATTGCTTTCTTTGTCACTGACCCTGAAATTG
CTGTCCAATGGCAGTACTGACAATCTAGTGCATTATGGGCCTGGCACAGACACACAAAAATTTATTTCATGGTTTCCAATAGAAGTCTATGGGGAAATTC
ATAATTGAATTAGGAAATACGCTTTTCTCATTGGATTGAATCTGGTTCTATATCTGCCTTTGCTTATACAGCCAATTTAATACAATTCTTATTTATTTGC
CTTACCTTTTTGAAATACACTGTGAAACCTACCTAGAGTATTGTCAAGAATGTTGTTGTAAGTATTTAAACTGTTATAATATTACAGTTTTAGTAAAAAA
TCTACAGGTACAAAAAAAAAAAAAAAA
5' EST: CF781609
[+]taattaatcccccccccccccggcagttagtgagggtgcgattgtgtcggagacatgatgcagagacccggaatggtcggagtcgcgctaaaccaccagg
tcacagcgttccacacgctcagggctttgctgcaggacaggggctcagaaccctgtgttctcatagaggaaccgcaccccctgcccattaccggcctcac
tgcactgaatgatgaatgtgccggggaaggtaccagcaccggtcgcaccaggactgccagggaactgcagccagactctgccagcttatactctgcctcc
gtcactacatccagcgcactaagggcaggatctgcagcggggactaaagcccctacactggcacagatggcatcgcagaaccgcctttccagggtcacca
actccaccgctcagctcttactggtggaacagaacatgctgcagcacctaccgccggagcggccccttggcattcagctgaaggacagcattgaattcag
aaacatctgcacgcatatggctcttcaggaggaggaccgcaagtttgataaggatgtgaatgaggcccagcaatgcttaaggacaatcatcaccaatctg
atcactgcccttttagccctgaactcagagttttgtgccgctgccgccgaggatctgcgctccatactgaaaaagctcactgaagactgaacaaagatag
gactgggtatggaacaatagggaatatgaatgggaagccaggagtttgccataacctattacagttatttggccacaagggaacaattcataaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: