XB-CLONE-4357139
Clone Name: IMAGE:7029777
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Gene:
serpinc1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081366 [+]
ACCAGTTGACAAGGAACACGCTCAGTAGCAGAACTGCTCATTTACAGAAACAACTTTCTTCTCTCTCCGGGTTTGGCAAAGAACATTGAAGCACAGGCTC
AGGAGCAGGGATGTATTTGCTTTCGTTGTTGCTTCTCAGCCTCCTGGGCTCAGGGTACCTCCAGTCACAGAATGCTGACATATGCTTGGCAAAACCTAAA
GATATCCCTCTGACTCCCATGTGTGTCTACCGGAAAACTCTGGAGGCTGTTGAAGCAGAGGAAAAGGAGAAAGAACCCGCACAACAAGAACAGAAGGTTC
CAGAGTCGACTAACCCTCGTGTATATGAGCTCTCACAGGCCAATGCTAAATTTGCCATTGCTTTCTATAAAAATCTGGCTGACTCCAAGCAAAACACAGA
AAATATCTTTATGTCACCCCTGAGCATCTCTCAAGCCTTTACAATGGCAAAACTGGGTGCTTGCAATAACACACTGAAGGAACTTATGGAGGTATTCTAC
TTTGACACCATTTCAGAGAGGGCTTCTGATCAAATACACTACTTCTTTGCAAAGCTCAACTGCCGGCTGTTCAGAAAAGCAAACAAATCATCAGAACTCG
TATCCGTCAACCGCCTCTTCGGCGAGAAGTCTCTTACCTTTAATGAAACCTATCAAGATATCAGTGAGTTGGTGTATGGGGCTAAATTGTTGCCCTTAAA
CTTTAAGGAGAAGCCTGAACTATCCCGTGAAATAATTAATAATTGGGTATCCGATAAAACAGAGAAACGAATAACTGATGTGATCCCTGTGGGTGTCATC
ACCCCTGACACAGTATTGGTGCTGATAAATGCCATCTACTTTAAGGGACTATGGAAATCCAAGTTTAATTCAGAAAATACTAAAATGGAACAATTCTACC
CAGATGAAAGTAACCACTGCTTGGCTGCAACCATGTATCAAGAGGGCATATTCCGCTACAGTTCCTTTAAAGATGATGGAGTTCAGGTCCTTGAACTGCC
TTATAAAGGTGATGACATAACAATGGTGCTGGTATTACCTTCTCCAGAGACTCCACTAATGAAAGTGGAGCAGAACCTGACACTGGAAAAACTAGGGAAT
TGGCTCCAAAAGTCTCGAGAATTACAGTTATCTGTTTATCTCCCTCGATTCCGAGTGGAGGATTCCTTCAGCGTCAAGGAGAAATTGCAGCAAATGGGAT
TGGTAGACCTGTTTGATCCCAACTCGGCAAAGCTTCCAGGAATCGTTGCAGGAGGAAGGACAGACTTGTATGTGTCAGATGCATTCCATAAGGCATTCCT
AGAGGTCAACGAGGAAGGTAGCGAGGCAGCGGCATCTACGGCTGTGATTCTGACAGGACGTTCCTTGAACCTCAACCGAATCACATTCAGAGCCAATAGA
CCCTTCCTGGTGTTTATCAGAGAAGTTGCTATAAATTCTGTTTTATTCATGGGAAGAGTTGCTAATCCCTGCACTGAGTAGCACCATCTTTGGCATATAA
ACCTACAGTAGTCAAACACACTTCCTAAACTATTTGTGTTTGTTTTTAATTTGTTCCCATCCTATCTCCATCTAATACTGACTATAACAAGTATTTTTGT
ACACTTTCTGATAATGATGATTGTAGCTGCTTATCTTGGGAGGTTGTAATGCCTGTGCCAACCATACACATAATGGTGTTAGCGTAGGCAGCACTGCAGA
AAAAAATAAACCTGACACATAGCTTTTGTGGAATCCAAAAAAAAAAAAAAAA
5' EST: CF781615 [+]taattaatcccccccccccccaccagttgacaaggaacacgctcagtagcagaactgctcatttacagaaacaactttcttctctctccgggtttggcaa
agaacattgaagcacaggctcaggagcagggatgtatttgctttcgttgttgcttctcagcctcctgggctcagggtacctccagtcacagaatgctgac
atatgcttggcaaaacctaaagatatccctctgactcccatgtgtgtctaccggaaaactctggaggctgttgaagcagaggaaaaggagaaagaacccg
cacaacaagaacagaaggttccagagtcgactaaccctcgtgtatatgagctctcacaggccaatgctaaatttgccattgctttctataaaaatctggc
tgactccaagcaaaacacagaaaatatctttatgtcacccctgagcatctctcaagcctttacaatggcaaaactgggtgcttgcaataacacactgaag
gaacttatggaggtattctactttgacaccatttcagagagggcttctgatcaaatacactacttctttgcaaagctcaactgccgggctgttcagaaaa
gcaaaaaaaatcatcagaactcgtatccgtcaaacggcttctttccgcgagaagtctcttacctttttaatgaaaacctatcaagaatatccagtgagtt
tggttgtaatgggggctaaaattgttggccccttaaaacttttaaaggagaaagccttgaaccaatccccggtggaaataaattaaataaatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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