XB-CLONE-4357103
Clone Name: IMAGE:7029755
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Gene:
ccnb2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080491 [+]
CTGAGTCTGCGTGATCCTGGGCAGCGAGGGTTTTAGCTTCCTTGTCTTTCGGTTTTATCGGTTGGTTTTAAACGTCCTATTCCCGCAGATGGCTACTCGT
CGGGCTGCCGTTTCCCGTGAAGCAGATAATATTCTTGGGGGTGCAATGAGGTCCAAAGCTCAAATTAATACCAGAAGAGCTGCTTTGGGGGAGATTGGCA
ACAAGGTGACAGTGCGAGGAAAACCACCTTCAGTCAAGCCTTCCATTGTTGTGGCAAAACCATCAAAGGCAGCAACTAAAGGGGCAAATGTTAAGCCGAA
GCCTGTACCTGTAAAAACAGCAGTAGCTGAAGCTCCCCCAAAAGTGCCCTCCCCTCTGCCAATGGATGTGTCTATGAAAGAGGAAGAGCTGTGCCAGGCC
TTCTCAAATGCCTTAACCAATGTTGAAGACATTGATGCTGATGATGGTGGAAACCCTCAGTTGTGCAGTGGCTATGTGATGGACATCTATAACTACCTAA
AGCAACTAGAAGTCCAACAATCTGTGCGCCCATGCTATCTTGAAGGAAAAGAAATTAATGAGCGTATGAGAGCTATCCTAGTTGACTGGATTGTTCAGGT
GCATTCTAGGTTTCAGCTTCTTCAGGAGACTTTATACATGGGGATTGCAATCATGGATCGCTTTTTACAAGTTCAGCCAGTCTCCCGCAGTAAGCTTCAG
TTAGTTGGTGTAACTTCACTACTCGTTGCTTCCAAATATGAAGAGATGTACACCCCAGAGGTTGCCGACTTTGTTTATATCACTGATAATGCCTATACTG
CATCTCAGATCAGAGAAATGGAAATGATCATTCTTAGGGTCCTCAACTTTGACCTTGGAAGGCCGTTACCTCTTCACTTCCTAAGACGGGCTTCAAAATC
TTGCAGTGCTGATGCAGAGCAGCATACCCTTGCAAAATATCTGATGGAGCTTACACTCATAGACTATGAAATGGTCCACTTCAACCCTTCAGAAATCGCA
GCAGCTGCACTCTGCCTGTCGCAAAAAATTCTTGCTCAGGGAAGCTGGGGTGCTACACAGCACTATTACACAGGCTACACAGAAAGTGACTTGCAACTGG
TCATGAAGCATATGGCTAAGAACCTGACCAAAGTCAACCAGAATTTAACAAAGCATGTGGCTGTGAGGAACAAGTATGCTAGCAGTAAGCTAATGAAGAT
CAGCACACTGCCTCAGCTGATGGCTCCTCTGATCACAGAACTATCTGCAAGCCTTTCTTAGAACTGTTAAGTTACCCTTTCAAAGAGAACCACTAATTGC
ACTTTTAATGCTGCTGGCAAATGCTGCTGTTCCTGTATATATGTTTAAACTTGTTGTGTTTTTTTTATTTGACTTGTGAACTTTTGAGTACATCCAAATG
CTGTAATGTCAGTATATGAAATAAAATCTTCACTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAA
5' EST: CF781633 [+]taattaatcccccccccccccctgagtctgcgtgatcctgggcagcgagggttttagcttccttgtctttcggttttatcggttggttttaaacgtccta
ttcccgcagatggctactcgtcgggctgccgtttcccgtgaagcagataatattcttgggggtgcaatgaggtccaaagctcaaattaataccagaagag
ctgctttgggggagattggcaacaaggtgacagtgcgaggaaaaccaccttcagtcaagccttccattgttgtggcaaaaccatcaaaggcagcaactaa
aggggcaaatgttaagccgaagcctgtacctgtaaaaacagcagtagctgaagctcccccaaaagtgccctcccctctgccaatggatgtgtctatgaaa
gaggaagagctgtgccaggccttctcaaatgccttaaccaatgttgaagacattgatgctgatgatggtggaaaccctcagttgtgcagtggctatgtga
tggacatctataactacctaaagcaactagaagtccaacaatctgtgcgcccatgctatcttgaagganaagaaattaatgagcgtatgagagctatcct
agttgactggattgttcaggtgcattctaggtttcagcttcttcaggagactttatacatggggattgcaatcatggatcgcttttttacaagttcagcc
agtctcccgcagtaagcttcagttagtttgtggtactttccctactccgtgcttcccaaatatggaagagatgtacccccccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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