XB-CLONE-4357045
Clone Name: IMAGE:7029721
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Gene:
cfl2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084909
[+]
GACTCACAATAACCCCTCCTTATTGACTGGATCACTTGCCAAGCTTGGAAATCAAGGCAACCCCCCGGATATAACGCTCTTCTGACACAGGACAAAGCAT
GGCTTCTGGCGTAACTGTTAATGATGAAGTCATAAAGGTATTCAATGAAATGAAGGTGAGGAAGTCTTCCACACCAGAGGAGATCAAGAAGAGGAAGAAA
GCGGTACTGTTTTGTCTCAGCCCAGATAAGAAGGAAATAATAGTGGAAGAAACTAAGCAGATATTAGTTGGTGACATTGGTGAAGCAGTTCCAGACCCCT
ACAGGACCTTTGTCAACCTTCTACCACTCGATGACTGTCGGTATGGACTGTATGATGCAACGTATGAGACCAAAGAATCCAAGAAGGAGGACCTTGTCTT
CATCTTTTGGGCGCCAGATAATGCACCTCTAAAAAGCAAGATGATATATGCAAGTTCCAAAGATGCAATTAAGAAGAAATTTACAGGCATCAAGCATGAA
TGGCAAGTGAACGGATTGGATGACATTAAGGACCGCTGTACCCTGGCAGATAAACTGGGGGGCAACGTAGTGGTTTCACTTGAAGGAGTTCCACTAAAAT
GAATAATTCCCACCCTACCCTGCAGAAGTGAACCCATTCAAAGCTTTTGTTCTGACCATAGGCAAAGTTTTAGGTGTTTTATTTATTGAATTTCTTTGTT
CCCTCATTCTCATCCCTCAAAGCACTGTTTTTTTCTGAAAACGTTACCATGTAAACTTAAAGGCATGGAGCAGCATATACGTGGTAATGGCAGGGTTGCT
TTAATTATAGAAAGGCATCTCCTGTTGTTAAACCCTGATGAAGAGCCTTAACCACAACCAAGTCTAGCCATATCTGTAATAATTACTGGCAGAATTTTTG
TATAATACAGAATCTTGCAATTCTTGTGACCCAAAAATATTCAGCTGGGTTAGGCAGCCCAAAGGACAATGAGCAGGCGTGCCATGACCCTCCAGTACCA
GATAACACAAGCTGCCATGTTTGTTGAGTAAAGCTAAAAGGCAGTATTCGAGTATTGGTGCTACTTTTTTTGCACTTTTAATTCACTTTCAAGATAAGCG
TTTGTGAACTGCCCTGTTGGACATTAACTAGAGGAATAGAGGGCCCTTGCTAAGAGTTGTCTTCAAAATGAATTGCTAGTCAAAAAAAATTCTCATCCTG
TAACATCAGTTCTCATATTGCAATGGCAGTTCTTAAGATCATCTAGGCTAGTGCCAGAATTTTTAATTCTGGTTGATAGATTTATAAGTTATACAAGCTG
TATGATCTTCATTGTTGGCCTCCAGCAACCAACCATAATCCAGGATGACTGATGATCTAACTGGGTGCATGCATTAGCCAAACACGAAATGCACTGGGCA
GCAATTCCAACATATTCATATTGTGTCAATATATGGGCATTTAGTACTTAGTGACAACAGTGTCGGACTGGGCTTCCACCAGAGAACCTGACATGTAGGG
CCCACTTTCATGACTATGATAACGATAAATGTGTGTTATATATGCCTATAATGAGAATAAGAAAGGGTTGATTGGAATATTCCTTGGCTGGCACCAACTA
GGCTCTGGGCCCACTAGAAGATCCAGTCAAAAAAAGTCACTTCAGGTATATTGCCATAACTGCTACAGTACATTTCACTATAGGAAATGTAATATGTGCC
ATATATTCTGCCGTTAAATGCCAACCTAGAAGCTACATCTTCCCTATGGCCACATGGACATCAAACTGCCTGTCTCTTTAAATTTGCTATATTGCACCTT
TTCCCAATCTCATCAGGATATAATTTTCTGTTACACATGCAATATAAATGACTACAGCTCAGCTGCCAGCAGCTGTAAAAGGTTTTATTGCATCTGAAGC
AACGTGATATCCCCCAAGCTTACAATAGTACTGCAGTTGCTGGGATGCCTTCAGGAGATGAATGGACAGAAAAAGCTGCTAACTCCTCCAGCTGCTGTAG
GGTTTTTTATCAACAGTTGAGTCAATAAACTATGCCATAACTTTACATGAACCAAGACATTTATTGCTGATTCTGTGCTAATAAACCAAATATAAAACCA
TATATTTTAGCACCTATTTTTTAGACCAAAATTGTAATAAAATTGCTGTTCCAGCTATTAAATTTACTTGTCACATTTTCTCTTGGTGATGTGCCAAGGA
CATTTTCCAAACATATAAGGGAATATTTTCCGAGAAACAATCATAAACTAAACCAAAAACAAACCAATAATAAAAAGTTTTTACTATTATTATGATCATT
ATTGTTTGATGACACCAGAAACAATGTTTTGTGGAATTTTGGATAACTGTGCAAAATAGGAATAAACTTGTTGGATTATTTCAGACAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF781662
[+]taattaatcccccccccccccgactcacaataacccctccttattgactggatcacttgccaagcttggaaatcaaggcaaccccccggatataacgctc
ttctgacacaggacaaagcatggcttctggcgtaactgttaatgatgaagtcataaaggtattcaatgaaatgaaggtgaggaagtcttccacaccagag
gagatcaagaagaggaagaaagcggtactgttttgtctcagcccagataagaaggaaataatagtggaagaaactaagcagatattagttggtgacattg
gtgaagcagttccagacccctacaggacctttgtcaaccttctaccactcgatgactgtcggtatggactgtatgatgcaacgtatgagaccaaagaatc
caagaaggaggaccttgtcttcatcttttgggcgccagataatgcacctctaaaaagcaagatgatatatgcaagttccaaagatgcaattaagaagaaa
tttacaggcatcaagcatgaatggcaagtgaacggattggatgacattaaggaccgctgtaccctggcagataaactggggggcaacgtagtggtttcac
ttgaaggagttccactaaaatgaataattcccaccctaccctgcagaagtgaacccattcaaagcttttgttctgaccatagggcaaagttttagggtgt
tttatttattgaatttctttgttccctcattctcatccctcaaagcacttgtttttttctgaaaacgttaacatgtaaactttaaaggcatgggagcaac
atatacgtgggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: