XB-CLONE-4357017
Clone Name: IMAGE:7029706
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Gene:
gtpbp4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC085000
[+]
TTCCCTTCTACACTGAGCTCGGCCTGGGAGATCAGCGGCCGCAGCCATGGCGCTTTATAACTTTAAGAAGATCATGGTGGTCCCTCCCGCTAAGGACTTC
ATAGATCTGACTTTATCCAAGACCCAAAGAAAGACGCCAACTGTAATCCACAAACATTATCAGATCAACCGCATTCGGCAGTTTTACATGAGGAAAGTGA
AGTACACCCAGCAGAATTACCACGACCGCTTGTCCCAAATTCTCACGGATTTCCCTAAACTCGATGATGTGCACCCGTTTTATGCCGACCTAATGAATGT
CCTGTATGACAAAGATCATTACAAACTGGCACTGGGACAGCTCAACATCGCCAAGAATCTGATTGACAACGTGGCTAAGGATTATGTGCGGCTGATGAAG
TACGGGGACTCTCTGTATCGCTGCAAGCAGTTAAAGCGAGCGGCTCTGGGGCGAATGTGCACAATTATCAAAAGGCAGAAGCAGAGCCTGGAATACCTGG
AACAAGTCCGGCAACATCTGTCCCGTCTGCCAACCATTGACCCAAACACCAGAACTCTCCTCCTGTGTGGTTATCCCAATGTGGGGAAATCCAGCTTCAT
AAACAAGGTGACAAGGGCAGACGTGGAGGTGCAGCCATACGCCTTCACCACAAAGTCCCTATTTGTGGGCCATATGGATTATAAATACCTACGGTGGCAG
GTTGTAGACACCCCTGGAATCCTTGACCACCCCCTAGAGGAGAGGAACACCATAGAGATGCAGGCAATCACCGCCCTTGCTCACTTGCGTGCCGCCATTC
TCTATGTGATGGACATCTCCGAGCAGTGCGGCCACACCATAGAGCAGCAGCTGGAGCTCTTCAACAACATTCGCCCCCTATTTTCCAACAAACCTCTGAT
TATCGTCATGAATAAATGTGATATTAAGAGGGTCTCTGAACTCCCTGATGAGCAGAGGAAAATATTCCAGGATTTGGAAAGCGAAGGCCTGACTGTGATC
GAGACCAGCACCCTCACTGATGAAGGGGTGATGACCGTCAAAACTGAGGCGTGCGATCGACTGTTGGTTCATCGCGTTGAAACCAAAATGAAAGGCAATA
AGGTGAACGATGTACTGAACAGGCTGCACTTGGCCGTGCCATCAAAGAGAGATGAGAAGGAGCGACTGCCATTTATCCCTGAGGGCGCACTAGCCAGAAA
GAAGCGCATGGTTACAGATGCACCTAAAAAGAGACTGGAAAAAGATATCGAGGTGGAAATGGGAGACGATTATATCTTGGATCTGCAGAAATACTGGGAC
CTCATGAATCCATCTGAGAGAACCGACAAAGTCCCCGAAATTTGGCAGGGTCACAATATTGCTGATTATATTGATCCGGAGATCATGAAGAAACTGGAAG
AGCTGGAGAAAGAGGAGGAACTGCGGGAAGGCGCCGGGGAGTACGACAGCGATATTGAGAGCGAGGACGAAGAAATGACCGAAATCCGAGAGCTGGCACA
GCAGATCCGGGAGAAGAAAAAGCTGAAGATCCTCGAGTCAAAGGAGAAAGATATACACGCACCGCGACTGCCTAGAACTGTTAAAAAGGTGCAACGCAAG
TCCTTAGAGAAGGAGATGAGTTCCCTGGGCCTTGACATGACCGAGAAAGATCAGACACATTACGCAGCGCAGGCGAGATCGAGGAGTCTGCAAAGGAAGC
GCAAGCGTGACGAGTCTGAGCCGCCCGTGTCCGCCGCCCGCAGCAGAAGCAGCTCCAAGACACCGCGAGATCAGTCTGGCATGCGTGATGTCAAGATGGT
GAAAAAGGCTAAAAAGATCATGAAGAATTCCCAGAAGAAGGGCAATCGTTTGGGCAAGAGAGGAGAGGCCGACAGGCATGTGTTTGATCTGAAACCCAAG
CATTTATTAGCCGGCAAGAGGAAATCTGGCAAGACGGACAGAAGATAACAGATTAACAAGCAAAGCCCTCCATCCCAAAGGACCTTGTATACAGAAAGAA
CTAATCTATAAGGGTGCCATGCACTTAGCCAGCTCCCCATTCTCTCTGCTGTGCAAACCCCAGGCTGCAGCACCCATACTCACAGAATTCAGGTGCCAAG
ATCAACCCAAACTCTCTGCTGTAATGGGTGCCCGGATGCTGGTTCCCAGATACATGTTTGTGAATAATAAACCTGTAATATCTGTTCCAAAAAAAAAAAA
AAAA
5' EST: CF781676
[+]taattaatcccccccccccccttcccttctacactgagctcggcctgggagatcagcggccgcagccatggcgctttataactttaagaagatcatggtg
gtccctcccgctaaggacttcatagatctgactttatccaagacccaaagaaagacgccaactgtaatccacaaacattatcagatcaaccgcattcggc
agttttacatgaggaaagtgaagtacacccagcagaattaccacgaccgcttgtcccaaattctcacggatttccctaaactcgatgatgtgcacccgtt
ttatgccgacctaatgaatgtcctgtatgacaaagatcattacaaactggcactgggacagctcaacatcgccaagaatctgattgacaacgtggctaag
gattatgtgcggctgatgaagtacggggactctctgtatcgctgcaagcagttaaagcgagcggctctggggcgaatgtgcacaattatcaaaaggcaga
aacagagcctggaatatctgggaacaagtccggcaacatctgtcccgtctgccaaccattgaccccaacaccagaactctcctcctgtgtggttatccca
atgtggggaaatccagcttcataaacaaggtgaccagggcagacgtggaagtgcaaccatacgccttcaccacaaagtccctatttgtgggcccatatgg
attataaatacctaccgtgggcaggtttgaaaacccccctggaatcctttggaccacccccctagaaggagaaggaacaccctttgaggatgccgggcaa
tcaccgctccctgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: