XB-CLONE-4356903
Clone Name: IMAGE:7029639
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Gene:
mpp1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081365 [+]
CCCCGAGCCTATTCCTTGGGAGATAAAGGAGAAGAGGCAGAGGGGTTCGATAGTATAAACCGGTGCAGGTTTATACAGTGGAAACACCTAGGAAGGCGGA
ATACCTCGTCTCAGGATGTCAATGAAGTTTGAGCGCGCAGAGACCCCGTTGTGTGACAGTTCCAGTGTGAGGACTGCCCTGTCAGACCTGTATCTGGAGA
CCCTGCTGCAAAACCGGGGCTCAGCCAAGGGCTATCAAACCAGTGTCGGCTCTCACACAGAGGAGATTTACACAAATGGATCTGCAGCTTCAGGGACAGA
GGAAAAGAAGAGAGTACGACTTGTTCAGTTTGAAAGAGAAACCGAAGAACCTATGGGTATTACGCTCAAGGTAAATTCACAGCAGAGCTGTATAGTTGCT
CGCATCTTACATGGAGGATTCATTCACAGACAAGGTTCACTTCATGTTGGAGATGAAATTCTGGAAATTAACGGGAAAAGTGTCCGAAATCAAAGTGTGG
ATCATCTGCAGAGTGTTCTGAAAGAAAGCAAAGGGACCGTTGTTTTAAAAGTGTCACCATCACAGAGCAATCGCAAGCCCCCACTCCAGATGTATGTCCG
AGCTCTGTTTGATTACAACCCTCTTTCTGATACACTGATACCATGTCGCGAGGCTGGCTTGGCCTTTCATACTGGAGATATCCTTCAGATCATAAACAAG
GATGATTCCAATTGGTGGCAAGGACGTTTACAGGGTGAGGGGTCAGCTGGGCTGATTCCCTCTCCAGAACTTCAGGAGTGGCGTGTGGCAAGCTCCAGTA
CAACAAATCAGAATCCACAGAGTTGTAGTCCTTTTAGCAAGAAGAAGAAATGTAAAGACAAATACCTGGCAAAGCACAGTTCTATATTTGACCAGCTTGA
TGTAGTGTCCTATGAGGAAGTAGTATCTCTACCAGCTTTTACAAGAAAGACCCTTGTGCTGATCGGCGTAAGTGGGGTTGGACGCAGCCACATAAAAAAT
ACTTTATTAGCCAAATACCCAGAAAGATTTGTGTATCCAGCACCACACACCTCCCGTCCCCCTAAACGCGGAGAGGAGGAGAGTGGCTCGTATCACTTTG
TTTCAGCAGAGGAAATGAGCCGGGCAATCTCAGAAAATGAGTTCCTGGAATATGGCAGCTTTAGTGGATATATGTTTGGAACAAAGATCCAGACTGTGAA
GGAGGTTCATAAAGATGGGAAAGTTGCTATTTTGGATATTGAGCCACAGACTCTGAAGATGGTTCGTACAGCAGAATTGGCTCCCTTTATTGTGTTTATT
TCACCCACAGACAAAGAGGAGTCGGAGGCTCTGCAGAAGCTGCGTGCAGACTCAGATGTCCTTCGCTCCCGATATGCACAGCATTTTGACCTGACTCTAG
TTAATAATGGAGTTGAGCAGACAGTGCAGGATCTGTTGGAAGCTTTTGATGCAGCCTGTTCTTCCCCTCAGTGGGTTCCTGTTACGTGGGTGTACTAAAA
ACTTACTAATAGGTGAATCCCAAAAATCATTGATCCCATTCAGCCATGAAAACTTACCCTTGGCCCCTAAGAATTACTCAAGTTTACCACAAAAGTCTTT
CTTCCTATATAAAACACATAGGAGAACAGGGTAATCTCTTATCAACTGCAATAGAAAACCAGTTGTTTAGAACCGTGCTGTCAAGCTTTTGTGCTGATTA
TAATTATGGACAGCCTTGATCTAGATTAAGATAATCTGAGTCTTACAATGCATTTTGATTCCAGAATACTTAAACATTGCCCAAATTGAACAGATGACAT
CTGGGAACTTTTCCAAACATACCCACCCAGAGAAGCCAGTGACATCATTTATCCTAAACTGTTAGGACACAGCATAAGGTTGCCAGATCATGTACAAAAT
GGGCTAGTAGAGCTGCACTGATTGAATTTAATTTTAATGCAATATGTTTGCAATCAGCTCATCTCATCTGAATTTATAGTTTCACATGATTTGGTAACAG
TAACATAGAGGTCATTGTGCAGTGAAGTATGCCGTGTAAACTCTTAGAAAAAAAAAAAAAAAA
5' EST: CF781733 [+]taattaatcccccccccccccccccgagcctattccttgggagataaaggagaagaggcagaggggttcgatagtataaaccggtgcaggtttatacagt
ggaaacacctaggaaggcggaatacctcgtctcaggatgtcaatgaagtttgagcgcgcagagaccccgttgtgtgacagttccagtgtgaggactgccc
tgtcagacctgtatctggagaccctgctgcaaaaccggggctcagccaagggctatcaaaccagtgtcggctctcacacagaggagatttacacaaatgg
atctgcagcttcagggacagaggaaaagaagagagtacgacttgttcagtttgaaagagaaaccgaagaacctatgggtattacgctcaaggtaaattca
cagcagagctgtatagttgctcgcatcttacatggaggattcattcacagacaaggttcacttcatgttggagatgaaattctggaaattaacgggaaaa
gtgtccgaaatcaaagtgtggatcatctgcagagtgttctgaaagaaagcaaagggaccgttgttttaaaagtgtcaccatcacagagcaatcgcaagcc
cccactccagatgtatgtccgagctctgtttgattacaaccctctttctgatacactgataccatgtcgcgaggctggcttggcctttcatactggagat
atccttcagatcataaacnaggatgattcccattggtgggcaggacgtttacaggggtgaggggtcagctgggctgattccctctccagaacttcaagaa
tggcgtgtggcaacc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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