XB-CLONE-4356857
Clone Name: IMAGE:7029609
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Gene:
ncaph
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080490
[+]
CGGACGTATCTGGACCGTTACCTATCTGACGTCATCGCGAACCAACCGAAGGCGGGAAGTTTTTGAAACCTCAAAGCAAACCGGAAGTAGGAGATCAGGG
CAGACACGGGATAAGACAAAATGAGTACATCAACTCCTCAGTCAGGAGTGCGCAGAAAGCCAGCAACCCCAGACAGTGCTTTTCTGTCCCCAGCCACTAG
GCCTCAGCCCGTCAGTGCGGCTGGTACTCCCACTCTGCAGAACTTTACTAGCAATGATGATGAACGGGAAAGGAAACTTCGGCGTATGTCCAGGGTCATT
GACCTGCAGCTTAGCAATGCTGATTCCCCTGCATCTGCCATTTCACCAGCTCAAAGCAGGGGAATAGATACTCCAACATCTCTACTGCCCAAATTAAGCA
ACACGCAGATTTCAGATCATTATTCCACCTGCATTAAGCTGTCTCAGGAGAATAAAATAACAACTAAGAATGCTTTCGGGTTGCATTTGATTGATTACAT
GGGTGACATCTTGAAGCATAAAGACAGCGAATTAACTAACTTTAAAGTTGCAGCTGGGACTCTGGATGCCAGTGCCAAAATATATGCAGTGCGAGTCGAT
GCTGTTCATGCAGATGTCTACAAAGTGCTTGGTGGGCTAGGAAAGGAATCTCAGGCAACTGAGGACACAGACAACCAGGAAATAGATAATTCAAAAGATC
CTCAGGACAGAAAGAATCAGAAGAAGAGGAAACGTTCATATAAAACAATTGAGAGGAATCTGAATAACATAAACCGTTCAGAAACAGAACGCAAGAGTGA
GGTTGACCCACTCTTCCAGAAGACAGCTGCCTCTTTTGATGAATTCAGTACTGCTGGTGTCTTCCTCTCCACTCTGAAATGTCACAGTTATCACAGCGAG
TTGCGCTTTGATACAGACGTCAGGCCACTTTCAACAACAGAGGAGACAGAGCCACCTAGTCCTGGTTCTGCCGACAGTACAGAACTTAAACCCCTGATTT
TACAGTGTGTGGAAAAGCGGCCAATATGTCCATCCCTGTCTGGGTTCCGCTTCATGCAGTGGAACAGTGATGCCCAAAATGAGAACTTATCACTGTTAAT
GGACAAGTTTAAAAAGAGTGACCACGTCTTTGACATAAATGCAGAGGTAGAGGATGACTTTGCAGAGTCAGAAGCCCCGGTAGCTGATGATTTTGATGCA
GATGTTTGTGATGGGATGGATGCTGGAGATATCGGAGAGTTTGCTGAGCATCGTGAGGCTTGCAGGTCGGAACGAAAAAGACCACAACTAACTCAAATTG
GAAATGGTGACATTGGTACCATGTGCCTCCAACTGTCTTCGTGTCCAGGGGAGTATTCCTATTTCAGCCCCCGTACCATGTCCATGTGGGCAGGCCCCGA
GCATTGGCGTTTCCGTCCCCGTCAGAAAGCCAGCACAGACTCAGACCAACAGAAGGTCAAGAAGGGAAAGAAAGTATTTGAATTAAATTTTGAAGATGAC
GTTGACTTTGAAGTTCATTTTCGAAAAACCAGAGCTGCTACCACACTTACAAAGTCCACACTGGAAAGTCAGAATAAGAAGTCAACTACTCTGCCTGCAG
ACTTTCATTATGATCCCGACAACATTGCACGGATGAGTTTGAGACCAAAAGACAGGATAAGAAAGACAACTGTTCAAGACTCTGTTTCTGAGACTGAAGA
TGACATTGGTGAATATGATTACAATAATCCCAATGATACTTCCAACTTTTGCCCAGCCCTTCAAGCTGCAGACAGTGATGATGATGATGATGGGGCATTT
TTGGGGCCGGAGTCCAACAGTGCTGGCTTTTCTGCCGAGAACCAGTTGAACATCACAAGTTATGGCGAGTCGAATCTCGTGGCAGAACCACAGAAAGTTA
ACAAAATAGAAATTCATTATGCCAAAACTGCTAAGAAGATGGACATGAAGCGTCTTAAGAGCAACATGTGGAGTCTGCTAGCTAACTGCCCTGAGTCACA
GGAAGAGATGCCAAGTTCAAAAAAAGAAGAAATTGATGCTGCATTAATAACTGATGAGAGAATATTCAGCAGTGTCACCCATGACTTGCAGAAAAGGTTA
CCTCCTGTGATGGCTCAGAATCTCTCAGTCCCCCTGGCATTTGCTTGCCTCCTGCATCTGGCTAATGAAAAGAATTTAAAGTTGCAAGGAATGGACGATC
TATCAGATGTACTTATAATGCAAGATGACTAACTATTGGCATGGCCTCTGAATTCGAGTCTCCTGGAGGAATTGTCAAGAGCGAACAAATAACACCTTTA
AGAAAAGATATATATTTTTTTTTTTTTTTTAAAAATGACTTTGGCCTTTTTTGCATCTGTAGGAGGAAAAAAATGTAGTTTCCACAATGAAACTGAAAAT
ATTTTTCATATATATGTAAATAATGTATTTATCTCAGAATAATACATGTAGTTTTTTTTAATCCCTTCCCATTTATAGCAGCCAGGATAAAATAAAACCC
AAAATAAGCCAAAAAAAAAAAAAAAA
5' EST: CF781756
[+]taattaatccccccccccccccggacgtatctggaccgttacctatctgacgtcatcgcgaaccaaccgaaggcgggaagtttttgaaacctcaaagcaa
accggaagtaggagatcagggcagacacgggataagacaaaatgagtacatcaactcctcagtcaggagtgcgcagaaagccagcaaccccagacagtgc
ttttctgtccccagccactaggcctcagcccgtcagtgcggctggtactcccactctgcagaactttactagcaatgatgatgaacgggaaaggaaactt
cggcgtatgtccagggtcattgacctgcagcttagcaatgctgattcccctgcatctgccatttcaccagctcaaagcaggggaatagatactccaacat
ctctactgcccaaattaagcaacacgcagatttcagatcattattccacctgcattaagctgtctcaggagaataaaataacaactaagaatgctttcgg
gttgcatttgattgattacatgggtgacatcttgaagcataaagacagcgaattaactaactttaaagttgcagctgggactctggatgccagtgccaaa
atatatgcagtgcgagtcgatgctgttcatgcagatgtctacaaagtgcttggtggggctaggaaagaatctcaggccaactgaggacacaaacaacccg
ggaaaatagataatttcaaaaagattcctcagggacagaaaagaaatccgaaagaaagaagga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: