XB-CLONE-4356845
Clone Name: IMAGE:7029600
|
Gene:
riok2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC080511
[+]
GGTAGTATGCGTTCCACAGAAGCCAGGCTGTCAGCATGGGGAAGCTCAATGTACTTTTGTTGCGATATTTGTCAAGAGAAGATTTTCGTGTCTTAACTGC
GGTTGAAATGGGTATGAAGAACCATGAGATAGTTCCAGCAAGTTTAATAGCCTCCATAGCAAGCCTGAAACATGGAGGATGCAATAAAATTTTACGTGAA
CTGGTGAAGCACAAACTCATTGCATACGAGCGGACCAAAACCGTACAGGGTTACCGATTAATTTATGGTGGCTACGACTATTTGGCATTAAAGACCTTGT
CTTCTCGTGAAGTTGTGACTTCGGTTGGAAACCAGATGGGCGTTGGCAAAGAGTCGGATATTTATATTGTCGCGAATGAAGATGAGAACCAACTGGCACT
AAAACTGCATCGACTGGGAAGAACATCTTTTCGTAACCTTAAAAACAAGCGCGATTATCACAAGCACAGACACAAGATGTCTTGGCTTTACTTGTCCCGT
TTGGCTGCAATGAAAGAATTTGCATATATGAAGGCATTGTATGATAGAGGATTCCCTGTCCCCAAGCCACATGACTACAACAGGCATGCAGTTGTTATGG
AACTAATTAATGGCTATCCATTATGCCAAGTCCGACACATTGAAGATCCAGCTTCTTTGTACAGTGAATTAATGGATCTCATTGTCAAACTTGCCAATCA
CGGTTTGATCCATGGGGACTTCAATGAGTTTAATTTGATGCTAGAAGAAGAGGATCATGTAACAATGATAGATTTCCCACAAATGGTGTCCACATCACAT
GCAAATGCTGAGTGGTATTTCAACAGGGATGTTAAATGTATCAGAGATTTCTTTCTGAAGCGGTTTAACTATGAGAGTGAACTATACCCCACCTTTAAGG
ATATCCAGAAGGAATGTTCTCTGGATGTGGAAATTGCAGTTAGTGGCTACACTAAAGAGATGCAAGAAGATGATGAGCTTCTACAGCCAACAGGACCCAG
TGATGATGATGATGATGATGATGATGATGATGATGATGGTGAAACATCCTGCAAGCAGCCAACTGAGGAGGAAGGAGAGAAAGCTAAGAGTGTCAGTAGT
GCAACAGGCACTTTAGATGCTCCATCCAGCCTGTCTTGGGAAGACTCTGTGAAAGATACAGCAGATGAGAGTGGGAGTCTTCAAAGCTTAGACATGGCAG
AATTTACTAAAGCATTGGCAGAGCTTGAAGGGCATCCAGGTGAATATCAAACTACTGGTATCCCATGCTGCTTTCCACTTGAGGATGAAAGACTGATAGA
TACAAATACAATTTCAGAGAGAGGTGACAAATGTCAGAAAGATGGGGATGATCAACTTGGAGACCAAGACTCTGATGAATGCCAAGATGAATGCCCTGAG
CTTGTTAACCTGTCTGAATCTAATAAAGAATATAAACCATTCAGAGATGAAGAAAGTCTGGCTCATGTAAATGAGCACAGAACAAGAACTACAAGCGTGA
CTTCTGTAGGCACCGTCGGTAGCTGCTCTACAATTCCACCGGAAATGATAAAACAGAAGGTGAAGCGACAGTTAACGAAGCAGCAGAAGGCCTCTATTCG
AAGACGGCTTCAAAAAGGAGAAGCAAATGTGAATACGAAACAGCGCAGGGACAATATGTACAACATTAAATGGAGTGTAGATGCTGCAAGTTTTTGGGGA
TAACTGCAGGAAAGTCAACACTTTGCATGTGTGCAGTGATGCTGTGCAATAAAATATTTTATGAAAAAAAAAAAAAAAA
5' EST: CF781762
[+]taattaatccccccccccccggtagtatgcgttccacagaagccaggctgtcagcatggggaagctcaatgtacttttgttgcgatatttgtcaagagaa
gattttcgtgtcttaactgcggttgaaatgggtatgaagaaccatgagatagttccagcaagtttaatagcctccatagcaagcctgaaacatggaggat
gcaataaaattttacgtgaactggtgaagcacaaactcattgcatacgagcggaccaaaaccgtacagggttaccgattaatttatggtggctacgacta
tttggcattaaagaccttgtcttctcgtgaagttgtgacttcggttggaaaccagatgggcgttggcaaagaatccgatatttatattgtcgcgaattga
agatgagaaaccactggcactaaaactgcatcgactggggaagaaactcttttcgtaaaccttaaaaaacaagcgccgattatcccaaagccacagaaca
ccaaaatgtcctgggcctttaccttggtccccgtttgggctgcaatggaaaagaaatttttgcataatttggaagggcttttttaatgaactagaaggaa
ttcccttggtccccccaaggcccccatggaacgttcaaccgaggcatggcaggtttgtttattgtgaaacttaaattaaatggggctaacccgttttatg
acccaaagatcccgaataaattttgtgaagaatttcaggtttttctttttgtttttagttggaaatttattgggggattctccattttggtcccaaagct
gtgggcacatattttatgggctttgttattccccttgggtgagcacacttccccatggaatcatctaaaaatttgtgaagggcttggacaaggaaaagaa
gggatctccttgtttgaacagtagtgtgtttgaatttttttcccgtctctaaaag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: