XB-CLONE-4356747
Clone Name: IMAGE:7029533
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Gene:
fzd2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080489
[+]
GGACTTGAAACAGCCTGTAGAGGAGTAAGGAGGAGAAGCGGAGCACAAAGAGAAAGTGATCCGGATCAGAACCACAGGGAAAGCGGCCGAGGCAGCCCGA
TCCGACACAACCCAACCGAACCGCACGTCCTGCAAACTTGGCTGAACTTCCACAAACAAACCGGGGAGTGAAACCGGGGCAGGCTCCCAAACCGACCAGC
GAGTCGCTCGGAATCGTTCCGTCCAAAATGAACCGGACAGAGTGGAGGTAAGCGGCACGATCGCTGCTGGAACCCCGTAGGGTCGAACTGTTGTCCCGCT
CCAGCCTGCGACTATTGGGAAGCAGCTGCAACAGTCGCGCACAGTCTGCCCGCTTGTCGCTCAGGCAAAGCTACTCCATTGATTTGGCTCTCAGAACAGC
AGCCCCTTCTCTGCCTCAGCAATGCAGGGAGTCACCAGAAGCTCCAATCTGATTGTGTGCCATCTCTTTGCCCTGTGCCTCCTCAGCTTTGCCCAACTCC
ATGGAGAAAAGGGCATCTCTGTGCCAGAACATGGCTTCTGCCAGCCCATCTCTATCCCCCTCTGCACAGACATTGCTTACAATCAGACCATAATGCCCAA
CCTGTTGGGTCACACTAACCAGGAGGATGCTGGCCTGGAGGTGCACCAGTTCTATCCGCTTGTCAAGGTCCAGTGTAGTTCTGAGCTGCGTTTCTTCCTC
TGCTCCATGTACGCCCCAGTATGCACGGTGCTGGAGCAGGCCATCCCCCCATGCAGATCCATATGTGAGAGGGCACGACATGGCTGCGAGGCCCTAATGA
ACAAGTTTGGCTTCCAGTGGCCTGAAAGACTACGGTGTGAGAACTTTCCACGTCATGGAGCGGAGCAGATTTGTGTTGGACAGAACCACTCTGAAGATGG
AGGTCCGACTCTGCTAACCACCAGTCCACCCCACCATGGTACGCCTGGGCCACCCATCTATGCCACACTGGACCATCCATTTCATTGTCCTCGAGTGCTG
AAAGTCCCCTCTTACCTCAACTACAGGTTCTTGGGAGAGAAGGACTGCGCTGCTCCATGTGAGCCTTCCAAGAATGATGGTTTCATGTTTTTCAGTCAGG
ACGAGATCCGCTTTGCCCGCGTTTGGATTCTCATCTGGTCTGTGCTGTGTTGTGCATCCACCTTCTTCACCGTGACAACCTACTTGGTGGACATGCAGAG
GTTTCGCTATCCCGAAAGGCCCATCATATTCCTGTCTGGTTGCTACACCATGGTGTCTGTGGCCTACATTGCTGGGTTTGTGCTGGGCAACAAAGTGGTC
TGCAATGAAAATTTTTCAGAGGGCGGCTACAAAACTGTTGTACAGGGGACTAAGAAGGAAGGTTGCACTATCCTCTTCATGATGCTTTATTTTTTTAGTA
TGGCCAGCTCAATCTGGTGGGTCATTCTCTCCTTGACTTGGTTTCTGGCTGCTGGTATGAAGTGGGGGCATGAGGCCATAGAAGCCAACTCGCAGTACTT
TCATCTGGCAGCATGGGCTGTACCTGCCGTGAAGACCATCACTATACTGGCAATGGGTCAGATAGATGGAGACCTCCTGAGTGGGGTTTGCTTTGTGGGT
CTAAATAACATTGACCCACTCAGAGGTTTTGTGTTGGCCCCACTTTTTGTCTACCTCTTCATTGGAACCTCCTTTCTCTTAGCTGGCTTCGTATCCCTCT
TCAGGATCAGAACCATCATGAAGCATGATGGCACCAAGACGGAGAAGTTGGAGAGGCTTATGGTGCGTATTGGGGTCTTCAGCGTCCTCTACACGGTTCC
TGCCACTATTGTTATCGCATGCTACTTCTACGAGCAGGCGTTTAGAGAGCACTGGGAGCGTAGCTGGGTCAGCCAGAATTGCAAAAGCCTGGCCATTCCC
TGCCCCCTGCAGTATACCCCACGCATGACCCCAGATTTTACTGTATATATGATCAAATATCTCATGACCCTTATTGTTGGCATCACGTCAGGGTTCTGGA
TCTGGTCAGGCAAGACTCTACACTCCTGGAGAAAGTTTTACACAAGGCTCACCAACAACAAACATGGGGAGACCACAGTGTGAGGAAGGAGATGGATGGT
TAGAGGACACCAGTCCTTTATCACTGGACTACCACTGACTGTAAATTCCTACATGTATTTTATCTCTCTCCCATATTCCCGGGATCCCAAGCAGTCTTTA
CAGTTATCGGGTGAAAGGCCCTATAGAATACCATCATCAGGTACATCCCTTTGGATTTGTAATAAAACAAACTGTAAATAGTTTTTTGCCCCCATAAATG
TAATTATATATTTCTATTTAAAATATGGGGCAGAAAATTGAGAAACAATAGAAAAATGTTTGCGGGGGCAAATACTCATTCACCCCCCCCCCCTTTCAAG
TATTTTCTTGATTCAGCAGTGCCTTCTGTGTAAATGAAGTCCTATAGCCTCCAGTCCTCGGCCTGTATTTGCCCTTTAATACTGCTCAGTTCGACGTTAA
CCCTGAAAAAAGTGTTATTGGTACGAATTTCCTCTTGAACTTGAAGGTTTCATTTAGAAGACAACCAAAGAACTTGGGAGCCACTAAAGTTGCTTGTGAG
ACTTGATGGCCTAACACAGACTTTTTTGGGGAGAAGGGTCCATGGAATTTTCTTTGCTCCCAGTTATCCACATTGTGAGAACATACATGGACTTAGCCAG
TCCTTCATTGCTGTGTGTTGCAATGCATGCTATGAGAACTAATACATGGCTAGGGACAGACTTACATATGCAATCTGGACATTTTCTGAACACTGAATTT
AAGTATTAGAGGGGAGGTACCTTATTAACCCCTTTGTTTGCTGGAGATCAACTTTCCCTATCCCGGTGGGTCTTCGCTAAAGCCCGTCATGTTGCCGCTC
CACGGTTCCTCTTTGAATAATGATATGTTGACGTGTTTCTTTGTGTTTTTTTTTCTATGTGATCTTATTATTATTATTATTTTTGCCCCTTTGAAATAAA
AATATTTTTTGTTAAGAACAAAAAAAAAAAAAAAA
5' EST: CF781811
[+]taattaatcccccccccccccggacttgaaacagcctgtagaggagtaaggaggagaagcggagcacaaagagaaagtgatccggatcagaaccacaggg
aaagcggccgaggcagcccgatccgacacaacccaaccgaaccgcacgtcctgcaaacttggctgaacttccacaaacaaaccggggagtgaaaccgggg
caggctcccaaaccgaccagcgagtcgctcggaatcgttccgtccaaaatgaaccggacagagtggaggtaagcggcacgatcgctgctggaaccccgta
gggtcgaactgttgtcccgctccagcctgcgactattgggaagcagctgcaacagtcgcgcacagtctgcccgcttgtcgctcaggcaaagctactccat
tgatttggctctcagaacagcagccccttctctgcctcagcaatgcagggagtcaccagaagctccaatctgattgtgtgccatctctttgccctgtgcc
tcctcagctttgcccaactccatggagaaaagggcatctctgtgccagaacatggcttctgccagcccatctctatccccctctgcacagacattgctta
caatcagaccataatgcccaacctgttgggtcacactaaccaggaggatgctggcctgggagtgcaccagttctatccgcttgtcaaggtccagtgtaat
tctgagctgcgtttcttcctctgctccatgtacgccccaagtatgccacggtgcctgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: