XB-CLONE-4356733
Clone Name: IMAGE:7029524
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Gene:
arl11l.1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077037
[+]
GATTACAGAGTCTGAAGAGAGAGAGAGCAGACAGATCTCCAGTCAACTGTCTAAGAAGAGCAGGCAGATCCCCAGTGAAAGAATAAAGGGCAAGCAGATC
CTCAAGAGAGAGCACATCTCCTTATAAAAAGGACTTTTGAAAAGAAGCAGCATCCATGGGTCTTCTCTTTAGCACCCTCTACAAAGCCCTCATGGGCTTC
TCTGGCACCAAGGCTAAAATCATTATGCTGGGACTGGATGCAGCTGGCAAGACCACAGTCCTGTACAAGCTAAAGCTTAATGAGACCGTCTGTACAATCC
CCACCATTGGATTCAACGTTGAGACAGTGGAGCCCATCCGTAACGTGTCCTTTACTGTGTGGGATGTGGGGGGTCAGGAGAGGATCAGAGCACTTTGGAA
GCACTATTTTGTTAATACAGATGGCCTGGTCTTTGTTGTGGATAGTGCTGACTGGGAGAGGTTCAAGGAAGCTAGTCTGGAGCTGGAATCCATTCTGGAC
AATGATGAAATGAGAGGCGTCCCATTTCTGGTGATGGCCAATAAGCAGGACCTTCCTGGTGCCAGGAGACCAATGGAGGTGGCAGAAGAATTAGGCTTGA
TGAAGATACAAGGCCACCAGTGGCACGTGCAGGGCTGTTGTGCAGCTACTGGGGATGGACTTGTGGAGGGCCTGGAAGTGCTCACAAACTTGGTCAAACA
GTTCCAAAAGTCCAGGTCCTAATGACCAAGCTTCAGCTGCTACCTTGTTTTGCAGAGAATTGTAATCTGGGGAGCCATGTAATGCAGGTATTGGGTCCCC
TTTGTGTTCTCTTAAACACATATAATGCACTACTATATTCACACTATTATTACAGTGCTAAGATAATGGCTGTTTATTGTGCATTTGAGGAGAGACAGTG
GGACTGATAATGTAATTAGACAGCCATGATATATGGGAGAGCATCAACCAGACAAGCACAGGCGGACCCACAATGACTTGAATGCATCAGGATAAAAGCC
ACCCAATATTATGCTTCTCTACTGAACATTACTATACACAGTGATTTTAGATGTTGTTGAACTACAAATCTTGCCCTCTTCCAGAAGCCTTAGGAAGGCT
ACAGGGTGCCTGTGATTAGAAGCGTTTTTTATGTTATCTCTATCAAACATGAAATGTCTCTGGTTATCATTTCTGTTCTGTGATTGTAATTGTATAAATA
AAGTCATATTAATGTTTTTCTAATAAAATGTACACTTAAATATTGCAGAAAACAAAAAAAAAAAAAAAA
5' EST: CF781818
[+]taattaatcccccccccccccgattacagagtctgaagagagagagagcagacagatctccagtcaactgtctaagaagagcaggcagatccccagtgaa
agaataaagggcaagcagatcctcaagagagagcacatctccttataaaaaggacttttgaaaagaagcagcatccatgggtcttctctttagcaccctc
tacaaagccctcatgggcttctctggcaccaaggctaaaatcattatgctgggactggatgcagctggcaagaccacagtcctgtacaagctaaagctta
atgagaccgtctgtacaatccccaccattggattcaacgttgagacagtggagcccatccgtaacgtgtcctttactgtgtgggatgtggggggtcagga
gaggatcagagcactttggaagcactattttgttaatacagatggcctggtctttgttgtggatagtgctgactgggagaggttcaaggaagctagtctg
gagctggaatccattctggacaatgatgaaatgagaggcgtcccatttctggtgatggccaataagcaagaccttcctggtgccaggagacccaatggag
gtggcagaagaattagggcttgatgaagatacaaggccaccagtggcacgtgcaggggctgttgtgcactaactgggggaggaacttggggaagggcccg
ggaaatgctcacaaacttggtcaaacagtttcaaaagtcccaggtcctaatgaccaagcttcagctggctaacttgtttttgcagaagaattggtaatct
gggggagcctgttaatgggcgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: