XB-CLONE-4356577
Clone Name: IMAGE:7027887
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Gene:
arl15
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080149
[+]
CAGAGGGGTCAGTAGCAGCAAATGCAGACTTGCAGCGGTGGTTCGGCTCCTTTATTATTCTTTAACAAGCTGTGCCCGACCGAGCCCCCAGCCCAGCAGT
AAAGAACCCTACCCCCCCCCTCTGTTCGCTACAGGCAAAGGCTCAGGCTTCCACTGCATGAATCCATGGACTACATGTGTTTTCGAGCACTCTGTTGTAA
GGGTCCTCCGCCACCGCGGCCCGAATATGATATTGTTTGTATAGGACTGACAGGCTCCGGCAAGACGAGCCTACTAACACAACTGTGCAGCGAGAGCCAA
GAAAACATTGTGCCTACCACAGGTTTCAGCATAAAAGCAATTCCTTTCCAGAGTGCCATCCTGAATGTAAAAGAACTTGGAGGGGCCACCAACATCAGGA
AGTACTGGAGTCGCTACTACCAGGGATCCCAGGGTGTGATATTCGTGCTAGACAGTGCCTCGTCCGAAGATGACCTCGAAACTGCCAGAAACGAGCTCCA
TTCAGCTCTGCAGCACCCACAGTTATGCACTTTACCCTTTTTAATACTGGCCAACCACCAGGACAAGCCAGCAGCTCGCTCAATACAGGAGATCAAGAAG
TATTTTGAACTTGAACCACTTGCACGTGGGAAACGTTGGATTCTGCAGCCAACATCACTGGATAACATGGAGGCTGTGAAAGATAGCTTCTCAATGCTAA
TAAATTTATTAGATGAGCGAGAGCCAGAGCCAATTCAAATGTGAAGCTCTTGAAGGGGGACGTCGTCCATAGTGATCGCCGCGCTGGCTGCTGCTTCTTC
TCCAACAGAAACTGAACTTGAGTGGAATTTCAATTGTGTGTCTTTGGGCCAAGCTGGAGTTTAGGTTGGAAAATCTGATATGAGCGCTTTGATTTTTATG
CCTTACAGCTGTTTTACTTGGCGTTAAACCCAATAGGTAAATAAGGTCTGATGTTTACTTTGAGCAGCAATGCCACCTAGCCAGCCTCTGTTTCAGAGCT
CATCCTCTAAGCGCAGCTTGTAGGGCAAATAGCTGTGTCTTTATTGGGTAAAAAAAGCTTTGGAGACCCGCTCCACTTCACATCTCAACCTGTACCAAAT
ATTCTGCTGTGTATACTCCTGAGTAGCCTTGCCACATTTACAGTCTATAATACACATCTTTTATTCTTTAGTATGTGGCGGTGCCTAGGTGGATGGAAAA
CTTTATATTGGGGAGCCATTTTTCGTTTCTCTCCGTGTCTCTTCCTGGATCATGCTTCTCTCTACCCAAGTCATTTAGAAATCCATTTAGGTTGTTTAAC
CAGTCACTGAGTTCTTAACTAACCACTTTTTTTAACTTCTATTTCCCAAGCATCTGATCCATACTGAATGGAAATAAAGAGCTACATTACTTACCTTTGC
TCAGCATTGGACTGGGCTGCTGGGGCAAAACCCTGGTGGGCCTCGGCGGCCCAGACCCAATCCCGACAGGGCACTCCACTGACCTGCCAGTCTCCCTCCC
CTGACGCGGTGTAGGTAAATTTAATTTAAGCGCTCGGGCGAGGGATTTGGGCGGGTGTTGGGACGCCCCTGCTAGAGGGTGGGCGGAAGCCTCGGTTGGC
CCTGCACCCCCCAGTCCGACCCTGCCTTTGCTGGCAGTCATATTCTACTACAGTGCAGAGACAGCAAGGTGCTTTTATGTACAAGGGGCCACATAAGGAC
ATGGCTGCTCTATTTGCTAAAAAAAATAAATTAGAGCCATTCATTCATCTTTAACCCCCCCAATTCCTGTACTACTGAGCCCATTCTATGCCACTAGTCA
GCTAATCATTTCCTTTCCCCTCTGCCGTCGTCTTTCCAACAAATGACATATCATATTGTATAGTACTGTTTTTATAGACCTGGTTTTATTGAGGGATACG
GTAGCATTGAATTACCACATTTTACAAATTTTATTTTAGTTTTATTAGTATCTTTTTTTTTTTTCATTTATATATAAATGTCTGTAAATAACGAATTGCC
CGCTTGGGGGAAATGTTAGTTGTAACCTTGTTGTTAGCCTTCCACGTGAGAGCTGGTGTTTTTCTCTACAATCCGTAACAATAGCGACAGTGTTAATAAC
AAGAAAAGAATGAATAAAATACATTTCTAAACTGACATTTGCCAATGTAAACAAAGTTTTTTTTATGCTAGATATTAAGAAAATTTCAAAAAGCACATTT
TATGAATCTTAATGGAAAAAATATCTTGATGTATGCTCATGATCAATATGGGAAAGCGACCTCAATGGCTATCACTACAGAGTGTGTTAATATTAGTGTT
TGACCCGCCTACATGGGGCCCACGTGTGCTCACTGTCACTGTGCAAACCTGGTTATTTTGTATTAAAAAAGAACAATTATTGCTGTATTTTCTTTCTAAA
CTGTGAACTTTAAATGGCATCAATGTTAAAAAAACACAGCTAATTATATAAAATAATATTGTTTTTTCAAAGTTCTATGAAGGTTTTTTGTGCTGTTTGT
ACCTAAAATAAAAGATAGAGTAAGTTTTTCCGAAAAAAAAAAAAAAAA
5' EST: CF781896
[+]taattaatccccccccccccccagaggggtcagtagcagcaaatgcagacttgcagcggtggttcggctcctttattattctttaacaagctgtgcccga
ccgagcccccagcccagcagtaaagaaccctaccccccccctctgttcgctacaggcaaaggctcaggcttccactgcatgaatccatggactacatgtg
ttttcgagcactctgttgtaagggtcctccgccaccgcggcccgaatatgatattgtttgtataggactgacaggctccggcaagacgagcctactaaca
caactgtgcagcgagagccaagaaaacattgtgcctaccacaggtttcagcataaaagcaattcctttccagagtgccatcctgaatgtaaaagaacttg
gaggggccaccaacatcaggaagtactggagtcgctactaccagggatcccagggtgtgatattcgtgctagacagtgcctcgtccgaagatgacctcga
aactgccagaaacgagctccattcagctctgcagcacccacagttatgcactttaccctttttaatactggccaaccaccaggacaagccagcagctcgc
tcaatacaggagatcaagaagtattttgaacttgaaccacttgcacgtgggaaaagttggattctgcagccaacatcactggataacatggaggctgtga
aaagaagcttctcaatgctaataaattttattagatgagcgaagagccagagccccattccaatgtgaaagctctttgaaagggggacgtcgttccatta
c
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: