XB-CLONE-4356463
Clone Name: IMAGE:7027798
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Gene:
shmt2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080148 [+]
GCATGATCACACGTGTTCAGTGATCCATAAGGGGCAAGAGGCCGGAGTGGGGCTGCAGTGCTAGCCCGGGGACTAGTAGACATGCTTACGTTTTCTTTAC
GGCGCTTGGCACAGCCCCTGCGGAGTTGCTGCCATGTTCGCAGTCAGCACAGCCAAGCTTGGACACAGGCTGGAAACCAAGTCTGGACTGGACAGGAAAG
TCTGGCAGAGGGTGACCCTGAGATGTGGGACCTTGTACAGAAAGAGAAGGATAGGCAATGCCGAGGGCTGGAGATGATTGCCTTAGAGAATTTCTGTAGC
CGGGCAGCCTTGGAAGCCCTTGGTTCCTGCCTCAACAACAAGTATTCTGAGGGCTACCCAGGGAAGAGATATTATGGGGGTGCAGAGGTTGTGGACAAGA
TTGAATTACTGTGCCAGCAGCGGGCTTTGGATGCCTTTGACTTGAATCCAGAAAAATGGGGAGTTAATGTACAGCCATATTCAGGCTCTCCAGCCAACTT
TGCAGCATACACAGCTGTTCTACAGCCACACGATCGCATCATGGGGCTAGACCTTCCTGATGGAGGACATCTGACCCACGGGTACATGTCTGATGTGAAG
CGCATTTCTGCTACATCTATATACTTTGAATCCATGCCTTATAAGTTAAATCCAGCAACAGGGTTAATTGATTATGATCAGCTGGAGATGACTGCTCGCC
TCTTCCGACCAAAGCTTATCATTGCTGGAACCAGTGCCTATGCACGGCTAATTGACTATGCCAGGATGAGAAAGGTATGTGATGAAATGAAAGCCTACCT
TCTGGCTGATATGGCTCATATCAGTGGACTTGTAGCAGCTGGCGTGATCCCATCTCCCTTTGAACATGCTGATATTGTCACCTCAACAACACACAAGACT
TTGCGAGGGGCAAGGTCTGGGTTAATATTCTACCGAAAAGGTGTAAAATCTGTGGATAAGAAAACTGGAAAAGATGTACTTTATAACTTGGAAGACAAAG
TCAACTTTTCAGTGTTTCCTTCGATCCAAGGGGGGCCTCACAATCATGCAATTGCAGCTGTTGCCGTGGCTTTAAAACAGGCCAGTTCTCCTATGTTCCG
TGAATATGCAGTGCAAGTCCTGAAGAACGCCAAATCCATGGCCGCCGCTCTGCTAAGCAAGGGTTACACACTGGTGTCAGGAGGGACAGATAATCACCTG
GTTTTAGTAGACCTGAGACCCAAGGGCATTGATGGCGCAAGAGCAGAGAGGGTGCTGGAGCTTGTGTCAATAACCGCAAACAAGAACACATGCCCGGGGG
ACAAGAGCGCACTTACCCCAGGAGGGTTACGGCTAGGTGCCCCTGCGCTGACATCTCGGAATTTCAAGGAAGCCGACTTTGAAAAAGTGGTTCATTTCAT
TGATGAAGGTATCCGCATCGGACTTGATGTTAAGCGCAAAACAAACAAACTTCAAGACTTTAAGAACTTTCTTCTTGAGGACCACGAAACAGTTAATCGG
ATAGCAGACCTGCGTAAGCAAGTGGAGCAGTTTGCCCGTTCTTTTCCCATGCCTGGATTTGATGAGCGTTGAATAAAATAAAAAGATGATTGATGGTGTT
AAGAACAACAAACCATTCCACCCTATTTCTTCCTGCGCTTCCTGTTGCCTCATTGCATTTTCTGTTAATCCAGCCAATTTCTTATTGCATTCTATTCCTG
CATCATATATTTAGTTGTCTCTTATCTTTTCTTATAACGGAGTGACCCAGGACCCTTTTCTACTTGGCAAACCTTCTCTCCAGCAGCATCACTGCATGAA
GAACTAGGCAGCTAGATTTGTTTCATTGTCATTTCCGTGGTGCATTGGACTTCCATAATGAAATCTCAAAACTCCAAATCTGACATTTCAGTGTGGGGTA
AGGGGATTCATGCTGCAGCAGAGCATATTCAGGTAGACTCCAGAGACTGTTAGAGCAATAAACAAAAACATCATGGTACAGCGAAAAAAAAAAAAAAAA
5' EST: CF781953 [+]taattaatcccccccccccccgcatgatcacacgtgttcagtgatccataaggggcaagaggccggagtggggctgcagtgctagcccggggactagtag
acatgcttacgttttctttacggcgcttggcacagcccctgcggagttgctgccatgttcgcagtcagcacagccaagcttggacacaggctggaaacca
agtctggactggacaggaaagtctggcagagggtgaccctgagatgtgggaccttgtacagaaagagaaggataggcaatgccgagggctggagatgatt
gccttagagaatttctgtagccgggcagccttggaagcccttggttcctgcctcaacaacaagtattctgagggctacccagggaagagatattatgggg
gtgcagaggttgtggacaagattgaattactgtgccagcagcgggctttggatgcctttgacttgaatccagaaaaatggggagttaatgtacagccata
ttcaggctctccagccaactttgcagcatacacagctgttctacagccacacgatcgcatcatggggctagaccttccctgatggaggacatctgaccca
cgggtacatgtctgatgtgaagcgcatttctgctacatctatatactttgaatcccatgccttataagttaaatcccgcaacaggggttaattggattat
gatccgcctgggaaatgactggctccgcctctttccgaccaaaactttatcattggctgggaacccggtgcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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