XB-CLONE-4356443
Clone Name: IMAGE:7027782
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Gene:
ap3s1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077669
[+]
TGGTGCTGGTATCGATGTGAGGGCGACGTTCTAATGCGCAGCCCTGGAGACCGAGCCCGGGCATAAGGACCCGTGTGTGAGGCGAGCATTAAACTTCCGG
TTACCGGTGCCCGAGTGCTGACACTGTACCTGCCCCGCTGGTCTCTGGCAGTGCCTGCTACGCAGCATCATGATAAAAGCGATCCTCATCTTCAACAACC
ATGGCAAGCCCCGGCTATGCAAGTTCTACCAGCACTATAGCGAAGACACACAACAACAGATTATAAGAGAAACTTTCCACCTTGTATCCAAAAGAGATGA
AAATGTTTGCAACTTCTTAGAAGGAGGCTTATTAATAGGTGGATCTGACAACAAACTCATATATCGGCATTATGCTACCTTATATTTTGTTTTTTGCGTG
GATTCTTCCGAAAGTGAACTTGGCATTCTAGATCTTATACAAGTGTTTGTAGAAACACTGGATAAATGCTTTGAAAATGTGTGTGAGCTGGATTTAATAT
TCCATGTCGACAAGGTTCACAACATCCTTGCAGAAATGGTGATGGGAGGTATGGTACTAGAAACTAATATGAATGAAATTGTCACTCAAATTGATGCATA
GACAAAGCTGGAAAAGTCAGAGGCTGGCTTAGCAGGAGCGCCGGCCCGGGCTGTGTCTGCCGTAAAGAATATGAATCTTCCTGAGATCCCAAGAAACATT
AACATTGGTGATATCAGTATAAAAGTGCCAAACCTGCCATCTTTTAAATAAGATCATTCCCTTTACTTGAATTTGATAAAACGTTTACGAAATCAATGTG
GATAGCGGTTTTCTATTCCATAAAACTGAAGGATCTGTATAGACTGTTTGCGATATATCTGAAGTGTTGGACTCAGCTAGATGTTGCTGTTAATACTGCT
AAGGCTAGCTCAACCTGACTCCAGAATTACATTCCTACGCCCCTGTAAGGCATGTCCTATGTCTTGGCTGCTCCCATTTCCACAGAAAGTATTTCCAATG
TCTCTTCGTTGTTCCGTTTACATAGTTGAAGAATTCCATTAGATAATCTGAAAATTCCTTATTCTATTATGTTTCTATTATGTTTTCTCTTTTACTCAAC
ATAAGACACAAAAATCCACTTTTTAAAGTATCATTTGAGATTGAACATGAACACAGTTGTTTTTAATATCGTTATCTAGGAACTTCATGATATAGCTGTT
TTAACACTGTAAAACATATTGAGATGGAATGTCGTGGTGTAGTATTCTGTAAAATAAATATTTTATGACTGAAAAAAAAAAAAAAAA
5' EST: CF781963
[+]taattaatccccccccccccctggtgctggtatcgatgtgagggcgacgttctaatgcgcagccctggagaccgagcccgggcataaggacccgtgtgtg
aggcgagcattaaacttccggttaccggtgcccgagtgctgacactgtacctgccccgctggtctctggcagtgcctgctacgcagcatcatgataaaag
cgatcctcatcttcaacaaccatggcaagccccggctatgcaagttctaccagcactatagcgaagacacacaacaacagattataagagaaactttcca
ccttgtatccaaaagagatgaaaatgtttgcaacttcttagaaggaggcttattaataggtggatctgacaacaaactcatatatcggcattatgctacc
ttatattttgttttttgcgtggattcttccgaaagtgaacttggcattctagatcttatacaagtgtttgtagaaacactggataaatgctttgaaaatg
tgtgtgagctggatttaatattccatgtcgacaaggttcacaacatccttgcagaaatggtgatggggaggtatggtactagaaactaatatgaatgaaa
ttgtcactcaaattgatgcatagacaaagctggaaaagtcagaggctggcttagcaggagcgccgggcccgggctgtgtctgccgtaaagaatatgaatc
ttccctgagatccccagaaacattaaccattgggtgatattcagtattaaaagtggccaaacca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: