XB-CLONE-4356387
Clone Name: IMAGE:7027746
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Gene:
sytl2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080147
[+]
GAGCAGAACCAGAACCCCACTGAGCTGGGATCTCCCAGGAGCTGCTTGGCCACCATTTAATCAGCAGAAAATTAACCAATTAGCCCGGCCATCTGCAGGG
GGTTAATTACAAACTGGGCTGAGAGGCACATTCTGCTGGGGAAGCCACTGAGGAGCAGCAATGATGATGATCCATACAAAGCCCGGGGCTGAATGACCCA
ATATCCACACAGGAGACAGAAAGCTGCGTTTGGGAGCAATGGGCCATGTCTGCGCTATGTAACTTCCCATCCGCTCCAGATATTAGCGGTCCAGAACATG
GTTGCCCTGTACTAGCCAAACATCCCGAGTCCAAACTCTCCCCCCTCTCACAGACTTGGAGAAAGTGAATGCTGCCGGCTGCAAAGACTCAGCAGAAATA
GCTGCCCTCTGATTGGTCACGTACTTTCCTTTTTTTTCCCCGTCTGATATTGATTTTGAATCTCAAAATGATTGATCTCAGCTTTTTAACAGAAGCCGAA
CAAGAGGCCATCCTGCGGGTGCTCAACAGAGACGCTGAGCTCAAGAAAGCAGAGGAGGAACGTGTCCGGCACCTCTATGATGCCGTCGATGATGAGAATG
AACTAAAATACAAAAGCGGTCAATGGTTTTATGAAGCAAAGTCAAAAAGGCATAGAGATAAGATCCATGGAGCAGATCTGGTCAGAGCGTCAATGAGAAA
AAGAAAAGCGCCACCGGCAACTCTAGCTGAATTAAGTAGAAACTATAAGGAAAACTCCAAGAAGACCTGGGTCAGCAGTGTCAACAAGGATGTGTTTATT
CCTCCAGAGCTTTATGGAGTCATGGAAGACCAAGAGGAGGAGCAAGGTGGCTTTGTGCCGGTGAAGATCGAGCCCACGCAAAGTTCCAAAAGAGTGAGCT
TCTTATCCACAGACAAAAACATGGAAAGTTCGAAGAACGGGAATGTATCTCCATCAAGGCAAAGAAGAAATCCATTTAACTCTACCAATGAGGGAGACGA
TGACACAGAACTTGGAAAGGACACACACGAACCTGTAAACGGACCAGAAAGTGGAGACAAGGTGTCCTACCCAGATGTCTTGCCCCCAAAAATAAAATTT
GGTGTGAAGTTGCCTTACCCAGCACCAGGAGAAATCATCCGACTTTCATCAACCTCTCCCAATGCCTCTTTAACAAATGGCGGACAGATTCCAATACCAA
AGCCTAGAACAATGCCACTGAAGAAAACGGACTCTCTAGAACGGTCTTCTTCTGACCTCAAAAGGGAGGATTCCTTCGGTAGTTCTGGCAGACCTAAAAG
CATTTTAAAACGCAGGTCCAGTTCAAGCTCTACAGATTCTGAATCTATAAGATTGGCCCAGAAAGCTGATTTAAACAAAATTGGCTTGTCTACCCCCATT
CTGCAAGCAGAACCTGTTGATGAGTCCCCAGACAAACTAAAGCAGGTGCGGTTCTCTGCAAATGTTCACCAGAACCCTCCATCCCCGGGGGGAGAGTCGG
TCGGTGGCAAAGAAATTGGTGAATTTGGAATTCTCGAACCAGGTTTATCCCAAGCTTCGTTGGAAAATCGTGCATCTTCTGAAGATTCGGTGTCGGCCCC
GAAGCCTTCTTCTAGTCTGGTTCTAGACATCCTAACAAAGACCATTTATCGAAAGGGCTCAGGGGAAATCCCAGACTTGAACGGGTCTCCAGTCGAAGGT
CCTAGTTTAGGTGCAAACTCGGGAAGTCTTAACGAAGACCTGAACAACATCAAAGAAGATTCATTTGTACAAGAAAACAAACCTGAAGTCAGTCTTGCAC
TGGAGGCAAATGTCTCTTCAGATAATGATCCTCTTTATGCCGCGGTGAAGAAACCTTCTGGATCCTTCTCTTCAGGTCTTTCCCAAGATAAGAAGCCAGA
AAGTGAATCGCCTGATGCTTCAAAACGTAACTCCATAGACCCAAAGCAAGGAATTGAAGAGTCTGATCCGATTTTGAGGGCACTGAAAAGGAGCAGTACT
AAAACGCTTCCTCCCCTACCAGTGCAGGAGATTTCCCTTGAGAAGTCAAACCAAGACGACATTACTGTTCCGAAGGAGGATAGGGACCATACCTACGAAG
AGGTCCGTGCAGATAAAGTCGTTCCGGAGAGAAGTCATTTTTCCGATCCAGAGAAACTAAAGCGACTGAGCCAATCGGTGCCCTCCTTCCTCAATGACGA
TACTGATGGCAGAGAGACAGATTCAGCATCAGAAAGCAGTTTTCAGCTTGGCAGACACAAGAAGAGCCCCAGTTCTCTAACCAATCTTAGCGGCTCCTCT
GGCATGGCCTCCTTATCTTCTGTTAGTGGAAGCGTCATGAGCATCTACAGTGGAGATTTCGGCAATGTGGACATCAAGGGTAACATCCAGTTTGCTTTAG
ATTACGCCGATAACCTGCAGGAGTTTCAGGTCTTTGTCAGCCAGTGCAAAGATTTGGCTGCGGCGGACGTTAAGAAACAGCGATCGGACCCCTATGTGAA
AGCCTATCTACTTCCAGAAAAGGCAAAAATGGGAAAGAGAAAAACGGGAGTCAAGAAGAAGACTTTGAATCCTGTCTACAATGATGTTCTAAGGTATAAA
ATCAACAAGAACGCTCTCCTGAGTCAAACGCTAAACCTCTCGGTCTGGCACCATGACGTCCTGGGGCGCAACAGTTTTCTAGGAGAAGTGGATGTGAACT
TGGCATCTTGGGACTGGAGTAATAAGCAGATGAACTGGTACCCACTGCAGCCTCGGACACCCGCTGCTGGAATTGGGCTGGAAAACAGAGGGGAGATGAA
ATTGGCTCTGAAATATGTCCCAGAAGCCTTCACGGGTGCTAAGAATGCTGGGACGGGCGAGGTGCACATATGGCTAAAGGAATGCACTAATCTCCCCATG
CTTAGAGGAAACAAAATAAACTCCTTTATCAAATGTACGATCCTTCCTGATACAAGTAGAAAAAGCCGCCAGAAAACACGGGCGGTAGACAAGACCCCGA
ATCCCTATTTCAACCACACCATGGTTTATGATGGGTTCAGGAAAGAAGATCTAAAAGAAGCTTGTGTGGAACTTACAGTCTGGGACCACAACAAACTGAC
CAATCACTTTTTGGGAGGCTTAAGGATAGGCTTAGGCACAGGTAAGAGCTATGGAACCCCGGTGGATTGGATGGACTCCAATGCGGCCGAAGCAACCACG
TGGGAGAAAATGATCACCTCCCCGGATACATGGGTGGAAGCCATACTGCCGCTCAGGATGCTAAAGATGGCTAAAATGGCCAAATAATTGGCAGCAGCTT
CTCTTTCCAGGCGTTCATAGGCTATATTAGTACTATTATCGCTAATCTACCCAATATCTATGGTTATTTGTAAAATATTCAATCTAAACGGCAGCGGTAT
AATGCTGCTCTACACGGACCTCTTCTGAGCACTCTCTCTCTTATGCATTTAATGTATTTGTGATATTAATGGGATTCCAACTGAGTATTCTAGTAATCAC
TATGTTTGTCACAGGGGAAATAGGCTGATTAGTAATAGATGTATGATTTTAATAAAATGCATATTCTATCAAAGGCGACTTTCCTCCAGAAAACCCAAAC
ACGCTGCTATTATAATGTTCTGGCACTCCTATCAAGAACAAAACAACGGACCCTCCTGCTGCCTGCTGGCCCTACCACGTCTTGGGGCCCACGGACTTTA
CAATAAAGTGTATAGTATTGGTAAATATGACTTGCTAAGAAATACTTTACGTTTACATATCGGCATGACCGGGCCGCTCGCTTTTGCGGATGCCGAGCGG
CCGAGAACGCTTTTTTTTATTTTTCACGGATTTGATATGCAAAGCGAAAAGCCTTTCACTCGATGAATATATATGTATAATGGCTCCACGGGCGCTGTAT
AAAGATAAATGTTAATAAGGTTTAAATCATTTTAAAATAAAATGTAAATAAATGTTAATTATACAAATAAATTGATTACTTCTATAAAAAAAAAAAAAAA
AGAAAAAAAAAAAAAAAA
5' EST: CF781991
[+]taattaatcccccccccccccgagcagaaccagaaccccactgagctgggatctcccaggagctgcttggccaccatttaatcagcagaaaattaaccaa
ttagcccggccatctgcagggggttaattacaaactgggctgagaggcacattctgctggggaagccactgaggagcagcaatgatgatgatccatacaa
agcccggggctgaatgacccaatatccacacaggagacagaaagctgcgtttgggagcaatgggccatgtctgcgctatgtaacttcccatccgctccag
atattagcggtccagaacatggttgccctgtactagccaaacatcccgagtccaaactctcccccctctcacagacttggagaaagtgaatgctgccggc
tgcaaagactcagcagaaatagctgccctctgattggtcacgtactttccttttttttccccgtctgatattgattttgaatctcaaaatgattgatctc
agctttttaacagaagccgaacaagaggccatcctgcgggtgctcaacagagacgctgagctcaagaaagcagaggaggaacgtgtccggcacctctatg
atgccgtcgatgatgagaatgaactaaaatacaaaagcggtcaatggttttatgaagcaaagtcaaaaaggcatagagataagatccatggagcagatct
ggtcagagcgtccatgaaaaaaaagaaaagcgccacccggcaactctagctgaattaagtagaaactataagggaaaactccaagaaggacctggggtca
acagtgtcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: