XB-CLONE-4356339
Clone Name: IMAGE:7027706
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Gene:
maea
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080474
[+]
GAGCTGCTATGGATTGTAGCTGGCTGGCAGGAGCCGAGGAGCTGGGCGCTTAGACAGCGAGCCGGGGCTGCGGGCTGCCTTAATACATAAGAAAATCGGG
CCTGTCCCGCGAAAAGCGAGCCTTTCAGGCGCTGTGGGATGGCATTGATTACCCATGATGCTCAGGACTGTGGGCATGTAGCCTCCAGCTAATTCTGTCC
GGCCCCTCCCCCTTTCCAAGCCAAGATGGCGGTCCAGGAATCAGCTGCTCAGCTCTCAATGGCTCTGAAGGTTCAGGAGTACCAACTTTAAAGGTGCCAT
ATGAGACTCTAAACAAGCGCTTTCGGGCAGCCCAGAAGAACATTGACCGAGAGACAAGCCACGTCACCATGGTTGTGGCTGAACTAGAAAAGACGTTGAG
CAGCTGCTCGGCCGTGGACAGTGTGGTTAGCCTGCTTGATGGCGTTGTTGAAAAATTAAGTGTTCTTAAGAGAAAGGCTGTGGAATCGATCCAGGCAGAG
GATGAGAGCGCCAAACTGTGCAAACGGCGGATTGAACACCTGAAGGAGCACAGCAATGACCAAGTGGCGGCAATCAATATGTGGAAAAAGAAGCGTATGG
ACCGCATGATGGTGGAACATCTCCTGCGCTGCGGCTACTACAACACGGCTGTGAAGCTAGCTCGCCAAAGTGGGATCGAGGACTTGGTGAATATCGAGAT
GTTTCTGACTGCAAAGGAAGTGGAGGAGTCTCTGGAAAGGCAAGAAACCATGACTTGCCTGGCTTGGTGCCATGATAACAAATCAAGGCTTAGGAAAATG
AAGAGCTGCCTGGAATTCAGTTTGAGGATCCAAGAGTTTATTGAGCTGATTCGGCAGAATAAGAGGCTGGATGCTGTTAGACACGCACGGAAGCATTTCA
GCCAGGCAGAGGGGAGCCAGCTAGATGAGGTGCGCCAGGTGATGGGGATGCTGGCCTTTCCCTCCGACACCCACATCTCTCCTTACAAGGATCTTCTGGA
CCCAGCGCGGTGGCGAATGCTGATCCAACAGTTCCGTTATGATAACTACAGGCTTCATCAGTTGGGGAACAACTCTGTTTTTACTATAACACTTCAGGCG
GGCCTCTCGGCTATTAAAACACCACAGTGCTACAAAGAGGATGGGACCTCGAAGAATCCCGACTGCCCCGTGTGCAGCAAATCTCTGAATAAATTAGCAC
AGCCCCTCCCGATGGCACACTGCGCCAACTCCCGGTTGGTCTGCAAGATTTCCGGAGATGTCATGAATGAAAACAACCCTCCCATGATGCTCCCAAATGG
ATATGTGTATGGATATAATTCTCTGCTTTCTATTCGCCAAGATGACAAAGTCGTTTGCCCTAGAACCAAAGAGGTCTTCAACTTCTCCCAAGCTGAGAAA
GTCTACATCATGTAGGCGTGGGAACCTCCTCGTGCCTCTCGCCCCTCCCCCCACACCATGACCAGAGTGCTCGGCCGACAGCAGCATAAACCGCACAATG
GGAGGGGGGGCATCGGCGACTGTTCAGTGGTTGCATTCATCCTTTCTTGCAACCAAAGATTTACCAAGTAACAACAGTACATGCGTCAAGGAAACATCTG
CTTCCAAATACAAAAAACAAAAACATTTGTACTTGAAATTTACAGTTTTGATTGTGCGAGTTTTGTAACGAGTCGCGACTGGCGCTTCTGAAAACTTCTT
TAAGAATTTAGCCATTTTCTTTTTTTTTTTTAACACTTATTTGCTATAGAGACTTGCGATGCTTGGCCCGTTCCTCAAGTTCTGAGGTTCTAGGGTTCCA
CTTTTGATTTTTTAGAGGTCTTGGGATCATATGGTTAGCGCTAAAGCCTATTTTGCTTTGAGAATGTATTCACGGGGAAATCAAACCCATATTCTTCCAT
GTTGGACTTCCCAGAGCTTTGCCTACATTCAGTAACTGCACATAAGCAACAGCCGTGGTCCTGATCCAGTATTTTCTGGATACACAACGCGTTTTCAACC
AACAGCCGCAAAGGAAATTTTAGTCTTTGTAACCTTAAGGAAACTTTGCTGTAGAAATTTGTATTTAGTGCTTATTTTTGCATGTTGGTGTTCATCAACT
AATAAAATATTGTTCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF782015
[+]taattaatcccccccccccccgagctgctatggattgtagctggctggcaggagccgaggagctgggcgcttagacagcgagccggggctgcgggctgcc
ttaatacataagaaaatcgggcctgtcccgcgaaaagcgagcctttcaggcgctgtgggatggcattgattacccatgatgctcaggactgtgggcatgt
agcctccagctaattctgtccggcccctccccctttccaagccaagatggcggtccaggaatcagctgctcagctctcaatggctctgaaggttcaggag
taccaactttaaaggtgccatatgagactctaaacaagcgctttcgggcagcccagaagaacattgaccgagagacaagccacgtcaccatggttgtggc
tgaactagaaaagacgttgagcagctgctcggccgtggacagtgtggttagcctgcttgatggcgttgttgaaaaattaagtgttcttaagagaaaggct
gtggaatcgatccaggcagaggatgagagcgccaaactgtgcaaacggcggattgaacacctgaaggagcacagcaatgaccaagtggcggcaatcaata
tgtggaaaaagaagcgtatggaccgcatgatggtggaacatctcctgcgctgcggctactacaacacggctgtgaagctagctcgccaaagtgggatcga
ggacttggtgaatatcgagatgtttctgactggcaaggaaagtggaagagtctctgggaaggccaagaaaccatgactttgcctggcttggtgccctgat
aaccat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: