XB-CLONE-4356323
Clone Name: IMAGE:7027693
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Gene:
rbbp7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080146
[+]
CGACTGGTAGTCTTTCTTCTTCTTGTGAGTGTTTGGCGCGGGAAGCTGCAGCAGGACCGGCACAAGCGCGGAACACTGGGTTTTTAGTCACCGCGAGGCC
GGCGGCCCTTGCATCTGCACAGGAGTAGAGAAGATGGCGAATAAAGAGATGTTTGAAGATACAGTGGAAGAACGTGTCATCAATGAAGAGTATAAGATAT
GGAAAAAAAATACCCCATTTCTGTATGACCTGGTTATGACCCATGCACTTGAATGGCCAAGTCTAACAGTTCAGTGGCTCCCGGATGTGACTAGACCAGA
AGGAAAGGACTATGCGCTGCACTGGTTGGTCTTAGGTACTCACACTTCAGATGAACAAAATCACCTTGTAGTGGCTCGTGTCCAGATTCCCAATGACGAT
GCCCAGTTTGATGCCTCACATTATGATAGTGAGAAAGGAGAGTTTGGTGGCTTTGGTTCTGTTAGTGGCAAAATTGAAACAGAAATTAAAATTAACCATG
AAGGAGAGGTGAACCGCGCTCGTTACATGCCCCAAAATCCATGTATCATTGCAACTAAAACTCCTTCTGCAGATGTGCTGGTTTTTGATTATACCAAACA
TCCTTCTAAACCTGACCCTAGTGGAGATTGCAGTCCTGATCTCAGACTTAGAGGACACCAGAAGGAAGGCTATGGCCTATCTTGGAACTCAAACCTTAGT
GGACACCTTCTCAGTGCATCAGATGATCATACTGTTTGTTTATGGGACATAAGCGCAGGACCAAAAGAAGGCAAGGTAGTTGATGCAAAAGCCATCTTTA
CTGGCCATTCTGCAGTAGTAGAGGATGTGGCGTGGCACCTTCTGCACGAATCCTTATTTGGCTCTGTTGCAGATGATCAGAAGCTTATGATCTGGGACAC
AAGGTCAAATACCACTTCTAAACCCAGCCATTCAGTGGATGCGCATACTGCTGAGGTCAATTGCTTGTCCTTCAATCCATACAGCGAGTTCATTTTGGCC
ACAGGTTCTGCGGATAAGGTTAAGTGACTTTACTCCCTACTTACTCAATCATTTCCTTAAGAACTTAATTGAGACTTGTATCATAATTTTATTTTGTTCC
CTTTGTAGACTGTAGCTCTTTGGGATCTCAGGAATTTAAAGCTGAAGCTCCATTCCTTCGAATCTCATAAGGATGAAATTTTCCAGGTTCACTGGTCTCC
TCATAATGAAACTATTTTAGCTTCCAGCGGTACAGATCGAAGACTTAATGTCTGGGATTTAAGTAAAATTGGAGAAGAGCAGTCAGCAGAAGATGCTGAG
GATGGACCTCCTGAGCTTTTGTTTATACATGGAGGGCACACAGCAAAAATCTCTGACTTCAGCTGGAACCCAAATGAACCATGGGTGATATGTTCAGTGT
CTGAAGATAACATCATGCAAATATGGCAGATGGCTGAAAATATTTACAATGATGAAGAACCTGACATACCAGCTTCTGAGCTAGAAGCTCAAGGATCATA
GATCTTTTTCCTTTTTCTTATTCACACTCACTATTGCAGATGCTGCCACATTTGTGACGTGGTTAATGTTTGTGTAAGTTAATTTGCAATTCTGAGTACA
TGTATTGACATTTTCTTTGTAAATTGTATTGTAATATTCTTGCTCTTTGTATTGGATACCTGGGGGACTCGCTGAAGCGGCTGATGGAATAAAAAAAAAA
ATTAATAACAAAAAAAAAAAAAAAA
5' EST: CF782023
[+]taattaatccccccccccccccgactggtagtctttcttcttcttgtgagtgtttggcgcgggaagctgcagcaggaccggcacaagcgcggaacactgg
gtttttagtcaccgcgaggccggcggcccttgcatctgcacaggagtagagaagatggcgaataaagagatgtttgaagatacagtggaagaacgtgtca
tcaatgaagagtataagatatggaaaaaaaataccccatttctgtatgacctggttatgacccatgcacttgaatggccaagtctaacagttcagtggct
cccggatgtgactagaccagaaggaaaggactatgcgctgcactggttggtcttaggtactcacacttcagatgaacaaaatcaccttgtagtggctcgt
gtccagattcccaatgacgatgcccagtttgatgcctcacattatgatagtgagaaaggagagtttggtggctttggttctgttagtggcaaaattgaaa
cagaaattaaaattaaccatgaaggagaggtgaaccgcgctcgttacatgccccaaaatccatgtatcattgcaactaanactccttctgcagatgtgct
ggtttttgattataccaaacatccttctaaacctgaccctagtggagattgcagtcctgatctcagacttagaggacaccaaaaggaaagctatgggcct
atcttgggactcaaaactttagtggacaccttctcagtggcatcagatgatcatacg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: