XB-CLONE-4356315
Clone Name: IMAGE:7027689
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Gene:
castor2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082733 [+]
CCAAGTTGTTTGTTACAGTGTAGCACCAGCTGCTTTTTGCACTCTGGAGCTTGTGCAGCTCGAGTCTTCCTGAACTCATTCAGCTTTCCCCCTCTTCTTC
TTCTTCTTCTTCTCTTCCAGATCCCCCACTCCTCTGTCTCTTTCCTACTTGCGCCTCGCTCATCCCCTGCACAAAGAGAAGGGCACAATAACGCTAATTA
ATCCAAAAGCAGACAAGAAAAGAAAAACAAAATACATAAAGAAAAAAACAACGTTTACCCTAGATGGTCTACAAATGAGAAGACGAAAAAGATGGAACTG
CATATTCTAGAGCACAGGCTAAAAGTAGCCAGCATAGCCAAGGAAAACATCCAGTTATTTACCTATGGATTAATCAAACTTGCCTTTTTATCATCCAAAA
CAAGGTGCAAGTTTTTCAGTCTTACAGAAACACCGGAGGACTATACCATTATTGTGGATGAAGAGGGGTTTTTAGAACTCCCATCCTCAGAACACCTGAG
TGTAGCAGATGCAACATGGCTGGCCCTTAATGTGGTGTCAGGAGGTGGCAGTTCCTCTAGCTCTCAACCAATCGGAGTGACCAAAATTGCCAAATCAGTT
ATTGCACCTTTAGCAGACCAGAACATCTCTGTGTTCATGCTGTCAACCTATCAAACTGACTTTATACTGGTACGTGAGAGGGATCTGCCTTTTGTGATGC
ACACGTTGGCAGCAGAGTTTACCATATTGCAAGTGGTAAATGGAGAAACTGTAGCTGCTGATAACCTTGGAGTAACAAATGGATTTGTGAAACCCAAGCT
TGTTCAAAGGCCTGTTATTCATCCACTTTCCAGTCCCAGCAATATGTTTTGTGTAACTAGCCTGGACCCTTATACACTTCCGACTGTGACTACACTCCTA
ATGGATGTTATGTTTTACTCCAATGGAGTAAAAGATTCAGTGGTGGGCAGCGAGGAGCCTGGTCATATCCGCTTCTTTTCCTTTTCCCTCATTGAGGGCT
ATATCTCTCTGGTGATGGATGTACAGACACAGCAGAGGTTCCCTAGTAATTTGCTGTTTACAAGTGCGTCAGGAGAGCTTTGGAAGATGGTTCGAATTGG
CGGGCAGCCTCTTGGATTTGATGAATGTGGGATCGTTGCACAGATCTCAGAACCCTTGGCTGCTGCTGACATCCCAGCATATTATATCAGTACCTTTAAG
TTTGATCATGCACTGGTACCCGAAGAGAACATCAATGGTGTAATCAACGCTCTCCAAGTCAGCCAAGCAGAAAAACATTAGAATTTTTTAAACTTTTTTT
TTTTTCATTATCTGTGCAGTATTTCAAGAGGAGCTCAGTTTTGAGAGGTGCCATGAAGGATGAGAGCATAAAAAAAGAAATAACGCTCCAGCAAGTTTTC
GTTTCCTTTTAGTCATAATATGGTTTCTCTTCTGTTAGTTTTCAAAGTGATCTGTAGACTTTCTGCTAACTTAGAGAAAAAAAAAAAAAAATTAAAAAAA
AAAAATTAAAAAACAAAAAAACCGACAAACAAGACAAAAAAAAAAAAAAAA
5' EST: CF782027 [+]taattaatcccccccccccccccaagttgtttgttacagtgtagcaccagctgctttttgcactctggagcttgtgcagctcgagtcttcctgaactcat
tcagctttccccctcttcttcttcttcttcttctcttccagatcccccactcctctgtctctttcctacttgcgcctcgctcatcccctgcacaaagaga
agggcacaataacgctaattaatccaaaagcagacaagaaaagaaaaacaaaatacataaagaaaaaaacaacgtttaccctagatggtctacaaatgag
aagacgaaaaagatggaactgcatattctagagcacaggctaaaagtagccagcatagccaaggaaaacatccagttatttacctatggattaatcaaac
ttgcctttttatcatccaaaacaaggtgcaagtttttcagtcttacagaaacaccggaggactataccattattgtggatgaagaggggtttttagaact
cccatcctcagaacacctgagtgtagcagatgcaacatggctggccccttaatgtggtgtcaggaggtggcagttcctctagctctcaaccaatcggagt
gaccaaaattgccaaatcagttattgcacctttagcagaccagaacatctctgtgttcatgctngtcacctatcaaactgactttatactggtacgtgga
gagggatctgcctttttgtgatgcacacgttgggcagcagagtttaccatattggcaagtggtaaatggagaaacctgtacctgctgaataaccttggaa
ttaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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