XB-CLONE-4356265
Clone Name: IMAGE:7027658
|
Gene:
tvp23b
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC077019
[+]
GAAGTGAGCTGGTGAAGATTCCGGAAGAAGGGAGAGAATGCTGCGGCAGGACAGCAATGACGAGACAGAAGACGTTTCCCTGTTTGATGCTGAAGACGAA
GGAACTAGGGCTGCAAAGAAGAAGAAGATAAGGCACCCCATCGCTTCCTTCTTCCATCTGTTTTTTCGGATCAGCGCAGTCCTGGTGTACATGCTCTCTG
GGTTTAGCAGCAGTTTCATTGCCTGCATGGTGACCATCATATTACTCTTGTCCTGTGACTTCTGGGTTGTGAAGAACATAACAGGGAGGCTGATGGTGGG
CCTGCGGTGGTGGAACCAGGTGGATGATGACGGGAAGAGTCATTGGGTGTATGAATCCAGGAAGGCAGCTCAAGGAAAGAAATCCAACTCCGAAGCAGAA
TCCCGAATCTTCTGGTTGGGGCTCATCTCCTGCCCCATCATTTGGGTCATCTTCGCCTTCAGCTCCCTCTTATCCTTCAACTTGAAATGGCTGGCTGTAG
TTATCATGGGGGTAACACTGCAGGGGGCAAATCTGTATGGATATATCAAGTGCAAAGTTGGAAGTCGCAAGAATCTCGGCAGCATCGCTACCAATTACTT
CGGCACCCAGTTCCTCAGACAGACCTTTTCCAGAGAGAACCCAGCAGAATCTTGAACCCCGTTCCCTACCCGGATTTTTCGCTGACAAATAATTGGGGAT
GAGAATTTTTGAGCAAAACGGAGATCTGAAATCTTCCTATTAATTTGTTTTTTTTTCTGCTGCTTTTTCTGATTGCAGTTTGTAAGGTTCATACTGGGGG
GGGGTGGGGGTGTTGGACAAACCACGGACAATGAGGGGGACCCGGCCTACTCCTGTGTGATGGGCCGAAAAGAAATTAACACTAATCTGCTGCCCCGGCC
TTAATAAGTCACTGGGAACATTTCATCTGCTAAAAGCACAACTTCTGTTATGTCTTTGTTCCTTGGCGCGGCGTGTAAATATGTATTTATGGGTACAGGG
GGTGCAACACTTCATTATGGAGAGTGGGAAATAAAGGCTATTTCCTCAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF782052
[+]taattaatcccccccccccccgaagtgagctggtgaagattccggaagaagggagagaatgctgcggcaggacagcaatgacgagacagaagacgtttcc
ctgtttgatgctgaagacgaaggaactagggctgcaaagaagaagaagataaggcaccccatcgcttccttcttccatctgttttttcggatcagcgcag
tcctggtgtacatgctctctgggtttagcagcagtttcattgcctgcatggtgaccatcatattactcttgtcctgtgacttctgggttgtgaagaacat
aacagggaggctgatggtgggcctgcggtggtggaaccaggtggatgatgacgggaagagtcattgggtgtatgaatccaggaaggcagctcaaggaaag
aaatccaactccgaagcagaatcccgaatcttctggttggggctcatctcctgccccatcatttgggtcatcttcgccttcagctccctcttatccttca
acttgaaatggctggctgtagttatcatgggggtaacactgcagggggcaaatctgtatggatatatcaagtgcaaagttggaagtcgcaagaatctcgg
cagcatcgctaccaattacttcggcacccagttcctcagacagaccttttccagagagaacccagcagaatcttgaaccccgttccctacccggattttt
ccctgacaaataattgggggatgagaattttttgagcaaaacgggagatctggaaatccttcctaataaatttggttttttttttcggctgctttttttc
tgattggcagtttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: