XB-CLONE-4356261
Clone Name: IMAGE:7027656
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Gene:
tra2b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077018 [+]
GATAAGTTGCTCCGTGCCCGGAAGTACGCGTCATTTCCTGCATGTTCAGAGGCCGGCTGAAGTTATCGCTTTGGTAAACCTGCAAGGCAAGATGAGTGAT
AGCGGGGAGCAAAATTATGCCGATCGGGAATCAAGATCTGCATCAAGGAGTGGAAGTGCACGACAATCTGGCAAGTCTGCAAGTCAGTCCCCGAATCACT
CTGCTGCCCGGTCTCGTTCAAAAGAAGGATCCAGGCATTCCAGATCAAAAACCAGATCCAGATCTGACTCAAGGTCTAGATCAAGAAGAAGTTCTCGTAG
ACATTATACAAGATCACGCACTCGCTCTAGGTCTCGGAGGAGATCAAGAAGCCGGTCACACAGTAGAGACTATAGAAGACGTCGCAGCCATAGTCATTCT
CCTATGTCTACTCGGCGAAGGCATGTTGGAAACAGGGCTAATCCAGATCCTAACTGCTGTCTGGGAGTATTTGGTTTGAGTCTATATACTACAGAACGTG
ATCTTAGAGAAGTATTTTCGAAGTATGGTCCAATTAGCGATGTTTCCATAGTTTATGACCAGCAGTCAAGACGTTCAAGAGGATTCTCTTTTGTATATTT
TGAAAATGTTGACGATGCAAAAGAGGCAAAAGAACGTGCTAATGGCATGGAACTTGATGGACGACGGATACGTGTTGATTTCTCAATAACAAAACGACCT
CATACACCTACACCAGGAATATACATGGGAAGACCAACATATGGCAGTTCACGGCGCAGAGATTATTATGATCGGGGATATGACAGAGGCGGGTATGACG
ACCGAGAGTACTACAGCAGATCCTACAGAGGGGGAGGAGGTGGAGGTGGAGGATGGCGTGGAGGACAAGACAGAGACCAGTTTTCTAGGAGGCGGTCTCC
TTCACCATACTATAGCCGTGGTAGCTACAGATCTCGCTCCAGATCACGATCATACTCTCCACGTCGCTATTGAAACCTGGTTCATGATCACTTCTATGAT
GTGTTTTGGTAAACTCTACAAACTTGTAGGCTTTTTGTGCAGCCTTGAAGATATAATGCATGTGTTTTTATAGTCTGACTTGTGACTGCTAAAGATGTTT
ATATAGCTTAAGCAATTTTGTGTCACTTTACCCTCTTTACACCTTCAATATCTGAATTCAACAAGCTAAAATAAAGCAAAATTTGAGTTTTGTTTTTAAA
AAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CF782054 [+]taattaatcccccccccccccgataagttgctccgtgcccggaagtacgcgtcatttcctgcatgttcagaggccggctgaagttatcgctttggtaaac
ctgcaaggcaagatgagtgatagcggggagcaaaattatgccgatcgggaatcaagatctgcatcaaggagtggaagtgcacgacaatctggcaagtctg
caagtcagtccccgaatcactctgctgcccggtctcgttcaaaagaaggatccaggcattccagatcaaaaaccagatccagatctgactcaaggtctag
atcaagaagaagttctcgtagacattatacaagatcacgcactcgctctaggtctcggaggagatcaagaagccggtcacacagtagagactatagaaga
cgtcgcagccatagtcattctcctatgtctactcggcgaaggcatgttggaaacagggctaatccagatcctaactgctgtctgggagtatttggtttga
gtctatatactacagaacgtgatcttagagaagtattttcgaagtatggtccaattagcgatgtttccatagtttatgaccagcagtcaagacgttcaag
aggattctcttttgtatattttgaanatgttgacgatgcaaaagaggcaaaagaacgtgctaatggcatgggaacttgatggacgaacggatacgtgttg
gatttctcaataacaaaacgacctcatacacctacaccaaggaatatacattggggaagaccaacatatgggcagtttcaccggcgcagaagattattta
tgt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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