XB-CLONE-4356229
Clone Name: IMAGE:7027633
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Gene:
exosc6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080174
[+]
GGGTGTTTCGGCGCATTAGGCCTGTCCGTGCTCACGGTGGGAGGAAACACGTTGTAGGATAGTATCACCCAGTGCATAGCCATGCCGGGAGACAGCCGCC
GTATTCGTGGCCCCGAAGAGTCGCAGTCCCCTTTGCTCTATGTACTGAGTGAAGAAGGAGGAAAAGCCGCAGGGAGAAGGGGCCGCGGTCCGTCAGAGCC
CCGGCCGGTGTTTGTCAGGGCCGGCCTCCTGAGCCAGGCTAAAGGCTCCGCGTACCTGGAGGCCGGGAGTGGCGGCACTAAAGTGCTGTGCGCAGTTCAT
GGGCCGCGGGAGCGCGGGATGGGAGGGGAGAGAGCAGAGACCCGGGGCCGCCTGCTCTGTGACCTGCGCTGGGCTCCCTTCTCCCGCCGCGGACCCTGGT
CTGGCTCTTGCCCCGCTGGGCCCTCTCCCCGCCAGGCCGGGCTGCAACTACAGGAAAGTCTGGAACCGGCCGTGCGATTGGATCGCTACCCCCGAGCGGA
GGTGATAGTGTGGGTCCTAGTGCTGGAGGATCGCGGCTCTGCGCTGCCTGCGGCCGTGTCTTGTGCCTCGCTGGCTTTGGCCGATGCTGGGATAGAGATG
TTTGACCTGGCGCTCGGATGTGGGCTAAGCCGCGGCCCCGGTGGGGAGCTTCTGCTGGATCCGGATGATGATGAAGAGGAGGCTGGGAGTGGGGGCACCA
TGAGCCTGAGCCTCTTGCCAACCCTTAATCAGGTTTCTGGACTTATTAGCAGTGGAGAGTGGGAAGGCGAGAGCTCGGAGGAAGCGGTTAGACTCTGCAT
GGAAGGCTGCCAGAGATTGTACCCAGTTTTGCACCAGTGCCTAGTGAAAGCCACTAAAAGGAAAATACCAGTGCCACAGCCTGACACAAACTGATCCTCG
TAGCTTTGGGTGCAGCTCTCCTTTCTAAGGCAAACTAATAGTGCTGCAAAGCAGGCAATGTCTACTTTCTGCTCTATAGTGGATCGGGACCACCCGAAGA
CAAAGCCACCATCGCTGGCTGATCAAATGTATTGTTGAAGTGTTATATTCTGTATATGGTCAGCTGAAAATTATAGCTCTGTATTCATGTTGAATGTGAA
AGGCGTATAGTATCTATACAGAACTTGTTTGCCAGTCAATCTGCTTTAACAGCTGCTCTCTAAAGACAGAAATAACACTGATTTCAGTTTACTATGGCAT
TTAGAATATCCCTTTACTGGGAACTTACTGTACCAGCTACTGTCACTTATTTCCTTAAAAGGGAATCTCCACCTGGTTTCTGTTTAAAACTCCATTTTCA
TGGGGCAAAATTATTTTCTATAGACAGCCTTAGGCTTTTTTCCCTGCTGTAGATGATCATAGGCGTTTTTTTTTTGTTTTTTTTAAAAACAAACTATGCA
CTTGGCATTACCATAAAACATTAAAGGCTTCTATATGAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF782070
[+]taataatcccccccccccccgggtgtttcggcgcattaggcctgtccgtgctcacggtgggaggaaacacgttgtaggatagtatcacccagtgcatagc
catgccgggagacagccgccgtattcgtggccccgaagagtcgcagtcccctttgctctatgtactgagtgaagaaggaggaaaagccgcagggagaagg
ggccgcggtccgtcagagccccggccggtgtttgtcagggccggcctcctgagccaggctaaaggctccgcgtacctggaggccgggagtggcggcacta
aagtgctgtgcgcagttcatgggccgcgggagcgcgggatgggaggggagagagcagagacccggggccgcctgctctgtgacctgcgctgggctccctt
ctcccgccgcggaccctggtctggctcttgccccgctgggccctctccccgccaggccggggctgcaactacaggaaagtctgggaaccggccgtgcgat
tggatcgctaccccccgagcggaggtgataatgtgggtcctaatgctggaggatcgcggcttctgctcttgctgcgaccgggtccctgtgccctctctgg
gttttggcccaaacctgggaatagagatgttttgaacctggggcccttggaaaggggggctaaaacctgcgggccccaggtggggggagccttctggcct
gggattcccggaatgaaggaaaggaataatgaaagcatgggaagttgggggggcacctattaaatatcttgaagccctttattgcaaaaaccatcttaaa
ttcagggggttacctggaggaatataatttgtcaagcgtn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: