XB-CLONE-4356125
Clone Name: IMAGE:7029079
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Gene:
krt15.2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC081376 [+]
GGCACTGCGGGCTGAAACGCTGTAAGACACGCGTCGCTCTTGGAACCATCTCCCACCATCGCAATGAGCTATAGCCTCTCTCAGAGCAGCTCATCTAGAA
TGAGCAGTGGAATCTCCTCCAGGCTCTCTGATGGGATCTACAGAGCTGCTAGTATGCAAGGGGGTTATGGAGGATCTGGCGTTTCCATGTCCAGTGCCAA
GTTCACATCAGCAGCTGGGTCAGGGATGGGCTTTGGCTCTGGATTTGCCAGTGGGGCATCCGCTGGTAGTGGGGCCTTCTATGGAGCCAGCTTTAGTTCA
GGGGGTGGAGATGGGCTGCTGTCTGGCAATGAGAAGTACACCATGCAGAACCTAAATGACCGCCTTGCCAGTTACTTGGCTAAAGTGAAGACTTTGGAGG
TGGCCAACACTGACCTGGAGGCCAAGATACGTGATTGGTATTCTAAGCAAGGGTCAGTCGCTGTCAGTGAACAAAGCTTTGGAACCTTGTACACCTCTAT
TGGTGAACTACGTCAAAAGATTTTAGCTGCCACCATTAACAACTCAAAGCTTGTCCTGGAGATCGACAATGCAAGATTGGCTGCTGATGACTTCAAACTC
AAGTATGAGAATGAGTTTTCCCTGCGTCAGACAGTCGAAAATGACATCAGTGGCCTGAGACATATCTTGGATGACCTGACCATGACCAGATCTGATCTGG
AGCTCCAGATTGAAAGCCTAAAGGAAGAACTGATTTACTTGAAGGCAAACCATGAGGAGGAAGTCTCTGAGAAAAAGCAGCATGCAGCAGGAACGGTGAG
CGTGGAGTTGGATGCTGTTCCTGGTGTCGGCCTTCTAAACACACTGAATGACCTTAGGGAGCAGTATGAATCTATTGCGGATAGAAACAGACGGGAAGCT
GAAGCTTGGTTCCTCAGCCAGGTTGAAGGTCTACAGAAAGAAGTTGTCAGCAGCACCCAGCAAATACAGAGCACCAAGAGTGAAAGCACCGAACTGAGAC
GTGGTTTGCAGAGTCTGGAGATTGAGCTGCAGTCCCAGTTGAGCGCTAAAGCCGGGTTAGAGGCATCGTTGGCAGAGACAGAAGGTCGCTACGCTGCACA
GCTCTTCCAGATTCAGAACATCATTAGCGGCATGGAGGCTCAGCTTTCAGACCTTCGTTCAGACCTTGAGCGACAAAACCAGGAGTACAGGACTTTATTA
GACATCAAGAGTCGCCTAGAACAGGAAATTGCCACGTACCATCAGCTCCTGGAAGGACAGGGAGGCCTAATCGCAGCTGGAGCAAATAGCAGTTCAGGGT
CCAGCAGTGGAAGCACAACTACCCGGGTACATGCCATAATAAAGGAAGAATCTGGTGGAAAGGTGGTCTCTTCCACACTTCTCAAGTCTTTCTGAGATGT
TCCTGCCGACCAGAGACACCTCCAGCATGTGATTTAGGGGCAGAGAATAACGGTGTGAGCTGCTACGGAACCCTCTTTCATACCCAGGGGCTTCATACAG
AAACACAGAGAAGGCTTTGTGCTGTCTTCCCATTCTCAGACTATTAGACAAGAGCTCTCTTAAGGTCCCCATACACGGCCGATTCTAGCTGCCGATATGG
GTCCCTTAGACAGATTCGGCAGCTAATCGGCCCGTGTATGGGCACTACCGACGGGCCTGCCCGACTGATATCTGGCCTGAAATCGTCCAGATCTCGATCG
GGCAGGTTAGAAAATACAGTCGGATCGTTGATGCAGTCCCTGGACCCACTTTTCCTATTCCCGTCGTTATAATTCGATCGTTTGGCCTGGATTCTCCCGA
TATCGCCCACCAGTAGGTGGGGATATCGGAGAAGATCCACTCGCTTGGCGACATTGCCAAACGAGCGGATCGGCCCATGTATGGGGACCTTTACTGTGTG
TAACCCCATAAAGCGGAGAAGGTTTTACTAAAATATTGAATAAAATGATTTCAATGTAATAAACAAACATAACTACACTGATAATCTGCAAAACCTTGTC
ACTTGGTGGAGAATGTTCTTTCTAAGTCAAATAAAGGTCTTCATGGGTTGTGCAGATGTTGAAAAAAAAAAAAAAAA
5' EST: CF782122 [+]taattaatccccccccccccggcactgcgggctgaaacgctgtaagacacgcgtcgctcttggaaccatctcccaccatcgcaatgagctatagcctctc
tcagagcagctcatctagaatgagcagtggaatctcctccaggctctctgatgggatctacagagctgctagtatgcaagggggttatggaggatctggc
gtttccatgtccagtgccaagttcacatcancagctgggtcagggatgggctttggctctggatttgccagtggggcatccgctggtagtggggccttct
atggagccagctttagttcagggggtggagatgggctgctgtctggcaatgagaagtacaccatgcagaacctaaatgaccgccttgccagttacttggc
taaagtgaagactttggaggtggccaacactgacctggaggccaagatacgtgattggtattctaagcaagggtcagtcgctgtcagtgaacaaagcttt
ggaaccttgtacacctctattggtgaactacgtcaaaagattttagctgccaccattaacaactcaaagcttgtcctggagatcgacaatgcaagattgg
ctgctgatgacttcaaactcaagtatgagaatgagttttccctgcgtcagacagtcgaaaatgacatcagtggcctgagacatatcttggatgacctgac
catgaccagatctgatctggagctccagattggaagccctaaaggaagaactgattttacttggaagggccaaccatggaggaaggaagtctcttgagga
aaaaagcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: