XB-CLONE-4356093
Clone Name: IMAGE:7029045
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Gene:
tmem120b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082959 [+]
GCTGGGTCATGGAGTTAGGGAGGTGACGTAAGGTGCGAGCGTGGCTCCTGCGGCCGGGCTCACAGCGGCTTCTAACATGTCGCTACAGAAGTGCCAGGAA
GAATGGGGAGAGCTGGAGAAAGAATTCCAGCAGCTACAGGAAACACACAAGGTATACAAACAGAAGCTGGAAGAATTGAATGGCCTTCAGAATCTATGCA
GCAGTTATATTAATAAGCATAAGAGGCGTCTGACTGAGTTAAAAGGCAACCTTCATGGATATAAACATACCTCTAATGTTGAAGAAAAGGAACTCGTCCA
GCAAATAAACAGCACAATTAAAGAGAGGCACAATGCATTTTTCGACATGGAAGCCTATTTGCCAAAGAAAAATGGTTTGTATTTAAACCTGGTGCTTGGA
AATGTGAATGTCACTCTCCTTAGTAATCAAGCAAAATTTGCTTACAAGGATGAATATGAAAAATTCAAGCTTTACTTGACTATTATATTGTTACTGGGAG
CTATCACATGTAGATTTGTGCTTCATTACAGGGTGACGGATGAAGTATTTAATTTCTTATTAGTCTGGTACTACTGCACTCTGACCATTAGGGAAAGTAT
CCTCATCAGCAATGGTTCAAGGATAAAGGGATGGTGGGTATCCCATCATTATGTCTCCACGTTTCTTTCGGGAGTGATGTTAACATGGCCAGATGGACTC
ATGTATCAAATATTTCGAAATCAGTTTCTGGCATTTTCCATATATCAAAGTTGTGTACAGTTTCTTCAATATTATTATCAGAGCGGCTGTTTATATCGAT
TACGTGCTTTAGGAGAGAGAAATCATTTAGACCTCACTGTTGAGGGGTTTCAATCATGGATGTGGCGAGGCCTTACATTTCTACTTCCTTTCCTTTTTTT
TGGACATTTTTGGCAGCTGTACAATGCAATTACACTCTTTGGGCTGTCAAGACATGAAGAATGCAAAGAATGGCAGGTGTTTGTGTTGGCTCTCACATTT
CTCCTGCTCTTCCTGGGGAACTTCCTGACAACTCTAAAAGTTGTTCACACAAAATTTCAGAAGAACAAGCTAAAGAAGCCTTGAGCCTGGTGTAAGACTG
GGGCAAAAATATACTGGATGGAATATTTGCCCCTCCCCCATTGTGGACTTTAGCATGAGGGTGGGCTGGAAAGTGCAGGGGCTGTGCTTTGGGTCCCTGA
TCAGGCTATTACAGTGGGGGTTGGTATAATGGATTGGAAGGTGGGTGCATAGATGCCGGCCAGTTTCCCTCTGGGAGAGACTTTCGTACTTGCCATTTCT
AAGCTGCTTTTTTCCCCCCTCTGGAAAGGATACGCTCAGGCCTGCGGTACAGACCACTGTGTTCTACGGCTGCTAATGGAATTACTGACTCTACTACTAA
TCCCCATTAATCATTTTGGCCACATTGCATTTTGAATCACATATGCAAAGGTGGGTTTCCCAAACCAGTGTGTTATGGTTGGTACCATGTTTATATGGAA
GTACAGTAAGGGCACAGGCTGGAGGGAATGAAAAATAAATGTTTACTACTGCGAAAAAAAAAAAAAAAA
5' EST: CF782138 [+]taattaatcccccccccccccgctgggtcatggagttagggaggtgacgtaaggtgcgagcgtggctcctgcggccgggctcacagcggcttctaacatg
tcgctacagaagtgccaggaagaatggggagagctggagaaagaattccagcagctacaggaaacacacaaggtatacaaacagaagctggaagaattga
atggccttcagaatctatgcagcagttatattaataagcataagaggcgtctgactgagttaaaaggcaaccttcatggatataaacatacctctaatgt
tgaagaaaaggaactcgtccagcaaataaacagcacaattaaagagaggcacaatgcatttttcgacatggaagcctatttgccaaagaaaaatggtttg
tatttaaacctggtgcttggaaatgtgaatgtcactctccttagtaatcaagcaaaatttgcttacaaggatgaatatgaaaaattcaagctttacttga
ctattatattgttactgggagctatcacatgtagatttgtgcttcattacagggtgacggatgaagtatttaatttcttattagtctggtactactgcac
tctgaccattanggaaagtatcctcatcagcaatggttcaaggataaagggatggtgggtatcccatcattatgtctccacgtttctttcgggagtgatg
ttaacatggccagatggactcatgtatcaaatatttcgaaatcagtttctggcatttttccatatatcnaagttgtgtacagttttcttcaatattattt
atcagag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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