XB-CLONE-4356061
Clone Name: IMAGE:7029021
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Gene:
h2az1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077029 [+]
GAGACTGTTTCGAGTGCCAGAGCGCTAGTTTGGGTTTAAGGAGGAATTACAGGACTTGATTAAGATAAAAATGGCTGGTGGCAAGGCTGGCAAAGATACC
GGAAAAGCAAAGGCTACCTCTATCACCCGATCCTCCAGAGCTGGATTGCAGTTTCCAGTTGGTCGTATACATAGACTGCTGAAGAACAGAACTACCAGCC
ATGGTCGTGTGGGTGGTACAGCGGCAGTATATACAGCTGCTATCTTGGAATATTTGACTGCTGAGGTTCTTGAATTGGCTGGAAATGCTTCCAAAGATCT
TAAGGTGAAGCGTATCAGCCCACGTCACTTGCAGCTTGCCATCAGAGGTGATGAAGAATTGGATGCTCTTATAAAAGCAACCATTGCTGGTGGTGGAGTC
ATTCCACACATACACAAGTCACTCATTGGAAAGAAAGGACAGCAGAAGACTGTTTAGTCAATGTCAGAAACCCAAGAACTCGTCAGACTTGATCAGCCTT
CTTTCTGAAGAAAAGACTGGTTTTCACACTCATCCAAGACTGAGATTCCCATTTCAAAGCAGATGCTTGGTGGTTTTAATGTGCCCATGGCTTCCAAGAA
GCCAGTTCTGATGGACTCTGGTTTTACATACAGGTTTTTTTTATGCAAGTTGTTGGGAGATGTATAAACATGGAACAGCTGATGCTTGATTTGTTTAGTA
AATGAATTTAATAAATGTTTGGCTTAATGGGTATGTCATGTAATTGCATTTTATTTCTAGCTGTTAAGCTGAAATAGACCATCTGTGCAATACTAGATTT
TGAAATGTTTCTCCTGTTGTGTTAAATCATGGTATTAAAATACTGCGATTTTGTGGTTGTAATTTAAAAAAAAAAAAAAAA
5' EST: CF782154 [+]taattaatcccccccccccccgagactgtttcgagtgccaaagcgctagtttgggtttaaggaggaattacaggacttgattaagataaaaatggctggt
ggcaaggctggcaaagataccggaaaagcaaaggctacctctatcacccgatcctccagagctggattgcagtttccagttggtcgtatacatagactgc
tgaagaacagaactaccagccatggtcgtgtgggtggtacagcggcagtatatacagctgctatcttggaatatttgactgctgaggttcttgaattggc
tggaaatgcttccaaagatcttaaggtgaagcgtatcatcccacgtcacttgcagcttgccatcagaggtgatgaagaattggatgctcttataaaagca
accattgctggtggtggagtcattccacacatacacaagtcactcatttggaaagaaaggacagcagaaaactgtttagtcaatgtcagaaacccaagaa
ctcgtcagacttgatcagccttctttctgaagaaaagactggttttttcacactcatcccagactgagaattcccatttccaagcagatgccctgggggg
ttttaatggggccccatgggctttccaaaaaacccgttttctgaatgggacttctgggtttttacataacagggttttttttttatggccaaatttttcg
gggaaaaaggtaacaaaccttggtaaccaccccgaaagcctttgaattttggcctaagtaaaaagggaattttcaccaaaagcggcttggggcttaaatg
ggggaatcgcgccacgggccacctttgccctttttctatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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