XB-CLONE-4356015
Clone Name: IMAGE:7028982
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Gene:
pxmp2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077678 [+]
GACCTGTCTAAATCTTCAAGAGGTACTAAGAGTTCTAAATCAAAATGCCGGTAGCTTCTAAACCAGTACCGGGCAGAGCCCCGCTTCACATGGTATTGCT
GCTGAGGTACCTGCAGCTTCTGCACAGCCGCCCTGTACTGACAAAGGCACTGACTAGTGCTATTCTGTCTGCACTAGGAAACATTTTGTCCCAAACCATA
CAGAAGTGGCGCAAAGAGCAGAAGCACCCTCAGAATGTGGACCTTAGAGGACCACTTCGATTTGCTGTTTATGGCTTACTCTTCACTGGCCCACTGTCTC
ACTATTTCTACTTGTTATTGGAGCAGCTGGTACCTTCATCTGCACCCCTAGCTGGGCTACAGAGACTCCTAATTGAACGATTAATAATTGCACCAGCTTT
TCTACTTCTATTTTTCTTGGTGATGAATCTACTGGAGGGTAAAAATTTTACAAAACTAAATCAAAAGCTGAAAAGCAGTTATTGGCAAGCATTAAAGTTG
AACTGGAAAGTCTGGACACCCTTTCAGTTTATCAATATTAATTACGTACCAGTACAATTTAGAGTCTTGTTTGCCAACCTGGTGGCTTTCTTCTGGTATG
CATACCTTTCCTCAACCCGGAACTGACATTGAGACAAGCAGATACAGATTGCAGAAACGCAGTAGCTGAATGAGATAATTGTTTTTTGATTAACATAGAA
AATGTCACTGAATTCCTATAACTTATTAATCTAACACAATTTAAGTCACCACTAAACAGACTCATGTTTGCCATTACTTCAGGTGCTAATAAGGAGCTTA
TTACTGTAAGAGTCAGAAAACTGTATGCATTAACTTTAACTTTGTAATAGTGCTCAATACGAGCATTAAGAAAGTTTTAAGAATAAATATAGCCATGTTT
TGAAAAAAAAAAAAAAAA
5' EST: CF782177 [+]taattaatcccccccccccccgacctgtctaaatcttcaagaggtactaagagttctaaatcaaaatgccggtagcttctaaaccagtaccgggcagagc
cccgcttcacatggtattgctgctgaggtacctgcagcttctgcacagccgccctgtactgacaaaggcactgactagtgctattctgtctgcactagga
aacattttgtcccaaaccatacagaagtggcgcaaagagcagaagcaccctcagaatgtggaccttagaggaccacttcgatttgctgtttatggcttac
tcttcactggcccactgtctcactatttctacttgttattggagcagctggtaccttcatctgcacccctagctgggctacagagactcctaattgaacg
attaataattgcaccagcttttctacttctatttttcttggtgatgaatctactggagggtaaaaattttacaaaactaaatcaaaagctgaaaagcagt
tattggcaagcattaaagttgaactggaaagtctggacaccctttcagtttatcaatattaattacgtaccagtacaatttagagtcttgtttgccaacc
tggtggctttcttctggtatgcatacctttcctcaancccggaactgacattgagacaagcagatacagattgcagaaacgcagtagctgaatgagataa
ttgttttttgattaaacatagaaatgtcac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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