XB-CLONE-4356009
Clone Name: IMAGE:7028977
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Gene:
sdha
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080510 [+]
AAAGATAAGTGAAAAGTGGGAGGTGCAGTACGCAGACTGTACGGGAAGCGTGGCGCTTGTTCAGTCAAAGAGTTTAGAGTGGCATAGGGAACATGGCGTT
TCTAAACGTTGCATCGTCGCGGCTTCTCAGCAAAACACTGCAGTTAGGCGGTAGAGTACAGAATTGCACTGCAACTTGTACAGCAGCAACACGTAACTTT
CACTTCTCAGTATATGGAAGAAAGGACACTTCAGCAAAACTTTCTGATTCGATCTCAACACAGTATCCTGTAGTTGATCATGACTTTGATGCAGTTGTTG
TTGGTGCTGGTGGTGCTGGATTACGGGCTGCGTTTGGATTGTCTGAAGCTGGCTTCAATACAGCTTGCATAACTAAGCTATTTCCTACCAGATCTCACAC
TGTTGCTGCACAGGGTGGAATTAATGCTGCGCTGGGTAATATGGAAGATGATGACTGGAGGTGGCACTTCTATGACACGGTTAAGGGTTCAGACTGGCTT
GGTGATCAAGATGCCATCCATTATATGACTGAGCAAGCACCAGCTTCTGTCATTGAGCTGGAAAATTACGGAATGCCTTTCAGCAGAACTGAGCAGGGGA
AAATCTACCAGCGTGCATTTGGAGGTCAGAGTCTTAAGTTTGGAAAAGGAGGACAGGCTCACCGTTGTTGCTGTGTGGCAGACAGAACAGGCCATTCCTT
GCTGCACACTCTCTATGGAAGGTCTCTTCGGTATGATACCAGTTATTTTGTGGAATACTTTGCCCTAGACCTTTTGATGGAAAATGGTGAATGCCGGGGT
GTGATAGCCCTCTGTATGGAAGATGGATCAATACATCGCTTCAGGGCCAAGAACACCGTGATAGCAACTGGGGGATACGGACGTACATTTTTCAGCTGTA
CCTCTGCTCATACCTGCACTGGAGATGGTACTGCAATGGTTACAAGAGCTGGGCTTCCATGCCAGGATTTAGAATTTGTGCAGTTCCACCCTACAGGAAT
CTTTGGGGCAGGATGTTTGATAACAGAAGGTTGTCGTGGAGAAGGAGGAATCCTTATCAACAGTGAAGGTGAAAGGTTTATGGAAAGATATGCTCCTGTA
GCAAAGGATTTGGCTTCGCGAGATGTGGTTTCCCGTTCTATGACTATAGAAATTCGAGAAGGAAGAGGCTGTGGGAAGGACAAAGACCATGTTTATCTTC
AGCTCCACCACCTTCCACCCAGTCAGCTGGCATCCCGACTCCCAGGAATTTCTGAGACAGCAATGATCTTTGCAGGAGTTGATGTAACAAAGGAACCCAT
TCCTGTCCTGCCAACAGTTCATTATAACATGGGGGGGTATTCCCACCAATTATAAAGGGCAGGTGATTACCCATGTAAATGGTGAAGATAGAGTGGTACC
AGGACTTTATGCATGTGGAGAGGCTGCATCTGCTTCAGTACATGGAGCCAACAGATTGGGTGCAAACTCACTTCTGGATCTGGTGGTCTTTGGTCGTGCA
TGTGCTCTAAGCATTGCAGAGTCGTGCAAGCCTGGTGAACCAGTGCCATCAATTAAGGAAAATGCAGGAGAGGAATCTGTTGCAAATCTGGACAAATTAC
GCTTTACCAATGGAAGCACTAGGACTTCAGAACTTAGAATCAACATGCAGAAGACTATGCAGAATCATGCAGCAGTTTTCCGAACAGGTTCTGTGTTGAA
GGAAGGTTGTGAAAAACTGAGTGCCATCAATTCTACAATGGATGACATCAAGACATTTGACAGAGGCATTGTTTGGAACACTGATTTAGTGGAGACACTA
GAGCTGCAAAATTTAATGCTATGTGCACTGCAAACAATTTATGGTGCTGAGGCACGCAAAGAGAGTCGTGGTGCCCATGCAAGAGAAGATTACAAGGTAA
GAATTGATGAGTATGATTATTCAAAGCCCATTCAAGGTCAGCAGAAGAAAAGTTTTAGCGAGCACTGGAGGAAGCACACGCTATCTTATGTTGATGGGAA
AGGAAAGGTTAGCTTGGAATACAGACCTGTCATCGATACAACTTTAAATGAAGACTGTGTGTCTGTTCCACCGGCAATTCGTTCTTACTAAACTACAGTT
ATTCAGTTTGAAGTCAAATTATTCATCCAGCAGTGTATTAAAAATAATTGTGGATTTTCGTAGTTAACTTGCACACAATCTTGCATTGCAGAAATCCTGA
TTCTGAGGAATCCAGTTTTGCTGTTAATAATAAATGAAATGATGCATACTTTTTGTTGATATTGTGGATTTTGCATTGCTATTGTTCATGAGACACCCTG
CGGATACAGATCTTGTTTGTATCTGTTCTAGGTTTAAAAATAAAATGCTGTAAAAAAAAAAAAAAAAAAAATCTGTTGAAGCAAAACTGTAAAAATAAAC
CAAAAACTCAAAAAAAAAAAAAAAA
5' EST: CF782180 [+]taattaatccccccccccccaaagataagtgaaaagtgggaggtgcagtacgcagactgtacgggaagcgtggcgcttgttcagtcaaagagtttagagt
ggcatagggaacatggcgtttctaaacgttgcatcgtcgcggcttctcagcaaaacactgcagttacgcggtagagtacagaattgcactgcaacttgta
cagcagcaacacgtaactttcacttctcactatatggaagaaaggacacttcagcaaaactttctgattcgatctcaacacagtatcctgtagttgatca
tgactttgatgcaattgttgttggtgctggtggtgctggattacgggctgcgtttggattgtctgaagctggcttctctactgcttgcataattaagcta
tttcctaccacatctcacactgttgctgcgtatggtgtaattaatgctgcgctgggtaatatggaagcattatgattgggaggaggctcttctccatgac
ccggcttaagtggttcccaccggtctcggttaatccccaattgcccctcccccttaccttgacctgatgtccagttcccgcctttcaggctgtttattct
tgaacaaccaccgggaatggcctttttcagccaaaacctgaaacacggcgataaatttttattacgcgtcgttttttggcggggttctaaagcccttccc
tttttcttacaaaggaaagacctggggtcttatccgctatgttcgcccatcgcgtgccaataaacgaacccccgtgcccgctctcgtttgggtgtgccgc
tatttctttttattgggaacggggcgcttctcccccgtaaagtagagtatccgagtataattcncctgcgggctcccgatatttcttatatcccttct
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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