XB-CLONE-4355953
Clone Name: IMAGE:7028925
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Gene:
pcid2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080509 [+]
GGAGAGTAATTTAGTCTTTGCAGGAGCACAATTGGAAAAACTGAACGTTGAGTAACCAGAAGAGTACATGTGCATTCCTTATGTAACCGTATAAAGAAAA
AAAAAATCTATCTGGAGGAAATATGGCACACATTACCATAAATCAATATTTGCAACAGGTGCAAGAAGCTATTGACTCTAAAAATGGCTACAACTGTGCA
GACCTAGTTTCATTCAGACATCCTCATGTTGCAAATGCTCGACTACAGTTGTCTTCACCAGAGGAAAAGTGTCAGCAGGTACTCGAACCACCTTATGATG
AAATGTTTGCAGCACACCTACGGTGCATTAATGCAGTGTCCAACCATGACTTTGTTGAGGCATACAAATATCAAACGCTTGTTGTGCAATCTTTTCTGAA
AAGCTTTCAAGCTCATAAAGAAGAAAACTGGGCCTTGCCTATTATGTACTCCATCACACTTGACTTAAGAATATTTGCTAACAATGCTGACCAGCAGCTA
GTGAAGAAGGGAAAAGGTAAAGTGGGAGATATGCTTGAAAAAGCTGCTGAACTCCTGATGAGTTGCTTCAGAGTATGTGCAAGTGATACCCGTGCTTCTT
TTGAGGATTCCAAAAAATGGGGAATGTTATTTCTTGTGAATCAACTTTTCAAGATTTATTTCAAGATCAGCAAGCTCCACCTATGCAAACCTCTAATCAG
AGCAATTGACAGTTCCAATTTTAAGGAAGAATACACCATGGCACAAAGGGTCACATACAAGTATTATGTTGGTCGGAAATCCATGTTTGACAGTGATTTC
AAGAAAGCTGAAGAGTATTTATCATTTGCCTTTGAGCACTGTCACCGATCCAGCCAGAAAAACAAGCGGATGATCCTTATTTACTTATTACCAGTGAAAA
TGTTATTGGGTCATATGCCAGCAATTCAGCTGTTGAAAAAATATGATCTGATGCAGTTTGCCGAAGTAACAAAAGCTGTCAGTGAAGGCAACCTGCTGTT
ACTGACCGAGGCTTTGTCCAAGCATGAGACCTTCTTTATTCGTTGTGGTATTTTTCTGATACTAGAGAATCTGAAGATTATCAGCTATAGGAACCTCTTT
AAAAAAGTGTACCTTCTACTAAAAACTCACCAACTTTCTTTGGATGCATTCTTAGTTGCACTGAAGTTTATGGAAGTAGAAGACATAGATCTTGCCGAAG
TTCAATGTATTATAGCTAATCTTATTTATATGGGTCACATTAAAGGCTATATATCTCATCAGCATCAAAAGCTTGTGGTCAGCAAGCAAAATCCTTTTCC
ACCTTTGTCCACAGTCTGCTAGCTTCTGCAGTTTTTAAAGATGAATTGAATGCGAGAGAACTCCTATTCTTTTGAATATCATCATTTAGAGATATCAGAT
TTACCTTTTATAATGGCAACAACTTACTTTTTATTCAAACTACTAATAGCAAAAAGACAAAAGATACTTTTTTAAAAGGGATGAGAAAAAGGACACGCTC
TTACATTTTTTAACACCTTTTATAAGGTCCCCATTTTTGTAATGCTGCATGATGTTCCAAAATCAGCTGTAATGCTTTCCAGTTCCAGTGACATGATATA
AACTATATGCCTTATTGCCCGGGTGGGGAGAGTAAAGGTGTTCACTAGATTGTACATTATTTTTCTGTGTATAGATTCTCCTGCTGAATAAAGTAACTTT
TTTTTTGTATTAAGGAATGCTTGACAATTTTAAAAAAATAATGTGAAATATATTCATGCAGTATGTCAGAAGCCGTAGCTAAGATTACGTAAGCTTTTCA
TTTGTAGTTTGTACTGATCCTGGTAACCAAAAAAAAAAAAAAAA
5' EST: CF782208 [+]taattaatccccccccccccggagagtaatttagtctttgcaggagcacaattggaaaaactgaacgttgagtaaccagaagagtacatgtgcattcctt
atgtaaccgtataaagaaaaaaaaaatctatctggaggaaatatggcacacattaccataaatcaatatttgcaacaggtgcaagaagctattgactcta
aaaatggctacaactgtgcagacctagtttcattcagacatcctcatgttgcaaatgctcgactacagttgtcttcaccagaggaaaagtgtcagcaggt
actcgaaccaccttatgatgaaatgtttgcagcacacctacggtgcattaatgcagtgtccaaccatgactttgttgaggcatacaaatatcaaacgctt
gttgtgcaatcttttctgaaaagctttcaagctcataaagaagaaaactgggccttgcctattatgtactccatcacacttgacttaagaatatttgcta
acaatgctgaccagcagctagtgaaaaagggaaaaggtaaagtgggagatatgcttgaaaaagctgctgaactcctgatgagttgcttcaaagtaagtgc
aagtgatacccggtgcttcttttgaggattccaaaaaatggggaaagttattttcttggggaatcaactttttcaagatttattttcaagatcagcaagc
tcccccctatgcaaacctcctaatcagaacaaattggacagttccaatttttaaaggaaaaatacccccctgggccccaaaggggtccaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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