XB-CLONE-4355801
Clone Name: IMAGE:7028790
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Gene:
kif20a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084524 [+]
GTGAATTCGGAAAGTAGCAACAGTCGGGAATCACGAGTCTGCAGAGAGCTGCCTGTCCCGGGAGGCGGTGGTTTCGGTAATACAGGGTAATACCAAGGGT
CATTATAACACAGGTACAGGGCCTGAATAATCATGGTTAGCAATGTTGTATCCCCTGGCATGGGACTGCTCTCAGATGATGAGGATAACCCAGTGTTTGA
ATCTACTGCAGCTGAATGTGGGGCAGGAATCCGAAAAGCACTTCTGTCTGACTTCTCTGTCATCTCACCAGGACTGGGCCATCCTCCCCAGTCTATGCCT
GAGGAATCAAATGACAAAGTCATGGTTTATGTGAGGATTAGACCCTTTGTAGATGGGGAGTTGGAGAAGAAGGAGGACCAGGGCTGTGTTGAGGTGGAGA
ATGAGGAGACCCTGGTGTTGTGTGCTCCTAAGGATTCCTTTGCAATGAAAAATACAGAACGCGGACTGGGGCAAAGTGTGCACAAATTTACTTTTTCTCA
GCTATTTGGACCAGAAGTGGACCAGAAGCAGTTTTTTGATGGAACTATGCGGCAAGTGGTAAAGGATGCTCTGAACGGCCAAAACTGGCTTATTTATACT
TATGGCGTTACTAACTCTGGCAAAACCTTTACCATACAAGGGACTAGCAAGGATAGGGGTATTCTGCCTCGCTCTATTGCACTTATCTTTAACAGTGCCC
AGGATCGCCTGTATAAAAGCTCAGACCTCAAACCTCTTTCTAATGAGGTGATGTGGCTGGACAGCAAACAAGTGCGACAGGAAGAACTTAAAAAAGTTTC
ACTGCTGTCTAATCTGAGAGAGGAAGAACTTATGACCCCCCTAAAAAGAAGTGGTAATGAATCCCAGGTCCGGGCTGGCACAAGTGTAAGCTTTGACAGT
GGCATTGGGGGACTTTCTTCTACAAGTCAGTTTGTCAATCAGACTTGTAGCCAGCTAGAAGAGACCTGTTCACGATGGAATGACCAAGAAGTAGTATCGC
TTCAGGAGGCTGCCGACGTACAACTCTCCATCTGGGTATCCTATTATGAAATCTACAATGAATTTGTTTATGATTTACTGGACTTCATGCCTTCTGGTCC
GAACCGTAAACGAACCACATTGCGACTGTGTGAGGACAGAAATGGGAATCCATATGTGAAAGACCTCACCTGGATAAATGTACACAGTGCTGAAGAAGCT
TGGAAGATCCTAAGAGTTGGAAGGAAAAACCAGAGTTTTGCCAGTACACATCTGAACCAAAACTCAAGCCGGAGTCACAGTATCTTTTCCATTCGGATTC
TCCACATGCAAGGAAAAAATGATTTGACGCCTAAAATAAGCGAGCTTTCTTTCTGTGACTTGGCTGGATCTGAGCGCTGTAAGGATCAGAGAAGCGGTGA
TCGCCTCAAGGAAGCAACTAACATTAATACCTCTTTGCACACTCTGGGCCGCTGCATTACAGTATTGAGACAGAACCAACAACAAAAGTTGCGACAGAAC
GTGGTCCCTTTCAGGGACAGTAAGCTGACCCGCATATTCCAGGCATACTTCACTGGGCGTGGGAAGTCTTGCATGATTGTAAACATTAATCAGTGCGCTT
CTACATATGATGAGACTCTTTATGCCATGAAGTTTTCAGCCATTGCCAGCCAGTTGGTTCAAGCTCCACCAGTTAAAGTGAACATTCCGTCCATTCAGTC
ACTCATCAAGGAAAGCACCATGCTAGCCAATCGCAGTTACACAGATGAGGAGGATGCTGACGAAAATTCTGATGAAGAAAGCGAGGAAACGGATATTGCA
AAATTTAACAGAGAGGAGTTATTACAAGTTATTGAGAACATGAAGGAACTAATTGTAAAGGAGCGTCAGGAGAAGCTGAACATGGAGATACAAATCCGTA
AGGAAGTTTGTAGCGAAATGATGGATATGATGCAACAACAGCAGAAACAGCATAGTGAGAGCCTGGAAGATGAGCGAGAATTGTTAGAGGAGATATACGA
GGGTCGGCTGGAGAACCTTAAAGAATCTCTAACAAATTATTACAAACACGAATTGGAGGAAAGAGATGAGAGGATTGAGGAACTGGAAGCTGCTTTGAAG
GAAGCCATGGAAAGTGTCCATTCCATGCCAAGCCAGCCGAGACAAAAAAAAAAAAAAAA
5' EST: CF782284 [+]taattaatcccccccccccccgtgaattcggaaagtagcaacagtcgggaatcacgagtctgcagagagctgcctgtcccgggaggcggtggtttcggta
atacagggtaataccaagggtcattataacacaggtacagggcctgaataatcatggttagcaatgttgtatcccctggcatgggactgctctcagatga
tgaggataacccagtgtttgaatctactgcagctgaatgtggggcaggaatccgaaaagcacttctgtctgacttctctgtcatctcaccaggactgggc
catcctccccagtctatgcctgaggaatcaaatgacaaagtcatggtttatgtgaggattagaccctttgtagatggggagttggagaagaaggaggacc
agggctgtgttgaggtggagaatgaggagaccctggtgttgtgtgctcctaaggattcctttgcaatgaaaaatacagaacgcggactggggcaaagtgt
gcacaaatttactttttctcagctatttggaccagaagtggaccagaagcagttttttgatggaactatgcggcaagtggtaaaggatgctctgaacggc
caaaactggcttatttatacttatggcgttactaactctggcaaaacctttaccatacaagggactagcaaggataggggtattctgcctcgctctattg
cacttatctttaacagtgcccaggatcgcctgtataaaagctcagacc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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