XB-CLONE-4355777
Clone Name: IMAGE:7028759
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Gene:
yipf5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080486 [+]
CGGACACGTAGCAACAGGAGAGGCTGGAGGGGTGCAGCTGAGAGAGCCGGGGTGCGCGGCCTGATAGAGCTGCCAACATGTCGAACTTTGATAATTTCAA
CACAGATTTCTATCAGACCAGCTATAGTATAGATGATCAGTCTCAGGGCTACAACTACAATGCAGGCGGAGCACAACACAGCAAGCAATATGCTTATGAT
CCCTACAGTCAGCAAGGAGGTTTTATTCCACCAGAGATGATGAATCAACAGCAGCCATACACGGGACAGATATACCAACCTACACAAACATACACTCCTG
CAGCCACTGACTCTGTGTATGGGAGTACATTTGATGATGAGCCACCTCTTCTAGAAGAACTGGGAATCAACTTTGACCACATTTGGCAGAAAACTCTGAC
TGTGCTACACCCTTTAAAAGTAGCAGATGGCAACATTATGAATGAGACAGATTTGGCGGGTCCAATGGTCTTCTGTCTAGCATTTGGAGCCACGTTATTG
TTGGCTGGAAAGATACAGTTTGGCTATGTCTATGGGATCAGTGCAATAGGATGTCTGGGAATGTATTGTCTTCTGAATTTAATGAGCATGACGGGCGTTT
CGTTTGGCTGTGTATCCAGTGTACTTGGTTACTGTCTTCTTCCCATGATTATACTCTCCAGTTTTGCTGTAATCTTCTCTTTACAAGGAATTTTGGGCAT
TGTTCTTGCTGCCTTGATAATTGGTTGGTGCAGTTTCTCCGCCTCAAAGATCTTCATTTCTGCTCTGGCCATGGATGGACAACAGGTTCTAGTTGCCTAC
CCTTGTGCTTTACTTTATGGAGTGTTTGCTCTTATCTCTGTGTTTTGAGCATATTTTATTTTAATTTGGACTTTAGTGGGCCAACAGCTAATAAAGACCA
ATCCTGTCTCCCAGAAAGGACCAGAGAGCTTTGCTATCTCACATTCTAATGTCTCTGGGACAACAATGGAAATAACGAACATCTGGTTCGCAGCTTTTAT
ACAGAACTGTTAATACTGAAGGGACTCCCAATGTCTTCGGTGTTAATAAAATGCCTGGTTGTAAGGTAACAATGTAATGTTTGGTGGTGTTCACAGAAGG
AACATGCATGCTATGGTGATGTTTCACTGATAATAGCACAGTATGAAAAATTATTTACAGTAATAAAAAAAAAAAAAAAA
5' EST: CF782296 [+]taattaatccccccccccccccggacacgtagcaacaggagaggctggaggggtgcagctgagagagccggggtgcgcggcctgatagagctgccaacat
gtcgaactttgataatttcaacacagatttctatcagaccagctatagtatagatgatcagtctcagggctacaactacaatgcaggcggagcacaacac
agcaagcaatatgcttatgatccctacagtcagcaaggaggttttattccaccagagatgatgaatcaacagcagccatacacgggacagatataccaac
ctacacaaacatacactcctgcagccactgactctgtgtatgggagtacatttgatgatgagccacctcttctagaagaactgggaatcaactttgacca
catttggcagaaaactctgactgtgctacaccctttaaaagtagcagatggcaacattatgaatgagacagatttggcgggtccaatggtcttctgtcta
gcatttggagccacgttattgttggctggaaagatacagtttggctatgtctatgggatcagtgcaataggatgtctgggaatgtattgtcttctgaatt
taatgagcatgacgggcgtttcgtttggctgtgtatccagtgtacttgggttactgtctttcttccatgaataaaactctccaggttttggctgtaatcc
ttctcttttcaaggaaattttggggcattgtttcctggctggcctttgaaaaaatgggttgggtgccagatttcctcccgcccttccaaagaatcctttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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