XB-CLONE-4355769
Clone Name: IMAGE:7028754
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Gene:
p4ha2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080485 [+]
GCATAGGAGCGCTGAGAACACAGGAGCACCCAGCTTAGAGTGGCTGAGATTGAGCTGTGTTGGAAGATGGAGCTACATGCTATCTCATTAATCCTGCTTA
GTCTTTGCTGCATATGGGAAGTCCAGACGGAGGTCTTCACCTCAACAGGTCAGATGACCGATCTGATATTTAGGGAAAGAGATCTTTTACAATCTTTAAA
AGAATACATCAGGGAAGAAGAGGAACGTCTTTCTAAAATAAAACGCTGGGCATCTGAAGTTGATGAACTATCTAGCAGATCAACTGCTGACCCAGAAGGA
TATCTTGGGCACCCTGTTAATGCATATAAACTTGTGAAGAGATTTAACACTGACTGGTCATCCTTAGAGAACCTTGTGCTTCAAGACTCAGCCAAAGGAT
TTATTGCCAACTTCACGTTTCAGAGAGAATATTTTCCCACTGAAGAAGATGAAACTGGAGCGGCCAGGGCTCTGATGCGCATTCAGGACACATACAAACT
TGATCCTGATGTCATAGCTAAAGGAAAATTACCCGGAACAAATTATATTTCATCTTTATCTGCGGATGACAGCTTTGGCATGGGGAAGATTGCCTACCAT
GATGGTGATTATTACCACACAGTACTTTGGATGCAGCAGGCATTGAAGCAGATGGATGAAGGGGAGGATGCTGTAGTTAATAAAGTGGATGTCCTGGATT
ATCTCAGTTACACTGTCTTTCAGCTTGGTGATGTCCCCAGGGCACTTGAGCTTACTAAAAGGCTCTTGGCTCTAGATCCAAACCATGATCGAGCTGTAAA
CAATCTGAAGTATTTTGAGAAAATGCAGGAGAAACAGAAAGCAGAACTAAAGCAGAATGAGAGCACTAACACAGAGTCAGCCACAAGGCAACCAGGAGTC
TATAGCAGGCCATTGGACTACTTGCCAGAGCGGGATGTCTATGAAGCCTTGTGCCGGGGAGAGGGAGTCAAAATGAATCCACGAAGGCAAAGGAGACTTT
TCTGTAGGTATCATAACGGAAACAGAAGTCCTTACCTTATCTTGAGTCCAGTTAAAGTGGAAGACGAATGGGATAGTCCACGTATTGTCAGATATCTTAA
TGCCTTATCTGATGAAGAAATTGCGAAAATAAAGGAACTGGCAAAACCAAAGTTAGCAAGAGCCACAGTGCGGGATCCTAAGACTGGTGTTCTATCAGTG
GCCAACTACAGAGTATCTAAAAGTGCATGGCTTGAAGAAAATGATGACCCAGTAATAGCACGTGTGAATCTTCGTATGCAGGCAATAACAGGGCTTACAG
TAGACACAGCAGAATTGCTTCAGGTTGCCAATTACGGCATGGGAGGGCAATATGAGCCACACTTTGATTTTTCAAGGCGTCCCTTTGACTCCAATCTCAA
AACGGATGGAAATAGACTTGCAACATTTCTTAACTATATGAGTGACGTCGAGGCTGGAGGAGCGACGGTATTTCCTGACTTTGGAGCAGCAATTTGGCCA
AAGAAGGGTACTGCTGTATTCTGGTATAACCTCTTCAGAAGTGGCGAGGGAGATTACAGAACACGACACGCAGCTTGTCCTGTATTAGTAGGTAGTAAAT
GGGGTAAGTGGACACATACTCAAGATCATCATTTTGATTCAGTTGTGTAATAAACCTCATGAGGCTCCAAGTGATTGGCAATGTACACATAAAGTTTGGT
CCTGGTTTCCCTTTTCAATTTCGCTTGCAGTCTGTTTTGATTCAGGAAATGTTCCTGAATCTTACCATTCGGAACAAATACTGGATCAAACAGACTTGGA
ACTGCAGAATCCTCTCTGCCAACTGATTTATTGTGCCATCATGCTTAACATGATTCAGTAGCCCCCCTTTGTCAAGTGATTAAATGTGTTGTGCATGATA
GCACACTATACACCTTTGCAGAGAAAGTTCTGCAGTTTGTCTGTTTGCTCTAAGCTATATGCAGTGTTATTGGGACACAGCCTGCAATCTTTATACAGTA
TAAATGCATACATTGGTGCTTTTCCTATTAATATTGATGTACCAGCCTGTAGATTGTATAAAGAGTTCTACAACTTTACCTGAAATCCTGTAATGCTTGT
GGAAGTCAATGATAGCCTGAGCCTATTCTGATCCATTGTGTTTCCATTCATATCTGCTTAAATCATAACCATTTCTATGACAAACCATTCCTCATTTCCG
ATATTAAATACTCATTATGTATATTTTTTTTTTGTTTTGTCAGTTTCCAATAAATGGTTCCATGAAAGAGGACAAGAATTCCTGAGGCCTTGTGGATTAA
CAGAAGTCGACTGATGTCCAAAAAAAAAAAAAAAA
5' EST: CF782300 [+]taattaatcccccccccccccgcataggagcgctgagaacacaggagcacccagcttagagtggctgagattgagctgtgttggaagatggagctacatg
ctatctcattaatcctgcttagtctttgctgcatatgggaagtccagacggaggtcttcacctcaacaggtcagatgaccgatctgatatttagggaaag
agatcttttacaatctttaaaagaatacatcagggaagaagaggaacgtctttctaaaataaaacgctgggcatctgaagttgatgaactatctagcaga
tcaactgctgacccagaaggatatcttgggcaccctgttaatgcatataaacttgtgaagagatttaacactgactggtcatccttagagaaccttgtgc
ttcaagactcagccaaaggatttattgccaacttcacgtttcagagagaatattttcccactgaagaagatgaaactggagcggccagggctctgatgcg
cattcaggacacatacaaacttgatcctgatgtcatagctaaaggaaaattacccggaacaaattatatttcatctttatctgcggatgacagctttggc
atggggaagattgcctaccatgatggtgattattaccacacagtactttggatgcagcaggcattgaagcatatggatgaaagggaagatgctggtattt
aaaaaagtggatgtcctggattatctcagttacacctgtctttcagcttggggatgtcccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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