XB-CLONE-4355721
Clone Name: IMAGE:7030250
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Gene:
csrp1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077045 [+]
GATAAACTCTGCAGACTGCCATATAAAGAGGAAACAGAGATCCGCTACCAAAAGAGAGCACACACTCTGAACAGGTTTCTTTTACCAAAGGAAGACAACA
AGCAGAAGAATGCCTAACTGGGGTGGAGGAAAAAAATGTACAGTGTGCCAGAAGTCTGTTTACTTTGCAGAAGAGGTGCAATGCGAGGGAGGAAGCTTCC
ATAAGTCCTGTTTCTTGTGCATGGTATGTAAGAAGAATTTAGATAGCACTACAGTAGCAATTCATGGAGAAGAGATATACTGCAAATCCTGTTATGGGAA
GAAGTATGGGCCCAAAGGCTATGGGTTCGGCCAGGGAGCAGGTACTCTAAGCATGGATCGTGGAGAGCACTTGGGCATCCAAACTGATGACCCCTCAAGA
TCCCAACCAACGAATAACCCCAATGCCTCCAAGTTTGCGCAGAAAGTGGGAGGTACAGACATTTGCCCTCGCTGTTCAAAATCTGTGTATGCTGCTGAAA
AAGTAATTGGTGCAGGCAATTCTTGGCACAGGACCTGCTTTCGCTGTTCTAAATGTGGAAAAGGATTGGAATCCACAACAGTGGCTGATAGAGATGGAGA
CATATTTTGCAAAGCGTGCTATGCAAAGAATTTTGGGCCTAAAGGTTTCGGGTTTGGTCAGGGTGCTGGAGCGTTGGCCCACACAAAGTGATGACGCTGG
AATTCTAAAAAGATGATTCTCTTTTCTGGCTTTTATCATTTTACTAACAAAGTTTGATATTTACAGCTGCTTTCTTACTATATCACAGTAAATATCTACT
GCAGTTTTAAAATATAAGCACATGTATTGACTTTTGGTAGCCTGTGGCCTGCTGGTATTAAAGTATATAAAGAAGCTACTTCAATCTCTAAGAAGGGATT
CTTGCTTTTCGCCACACAAATTCACATTTAGGCAAACTGTTTTCCAAAGCAAAACAGTGCACAATCACACTGAAACTACTGTGGATGTAAGAATATGAAG
GACAGATTTTATAACGCAATTGACTAACCAAAAAAAAAAAAAAAA
5' EST: CF782324 [+]taattaatcccccccccccccgataaactctgcagactgccatataaagaggaaacagagatccgctaccaaaagagagcacacactctgaacaggtttc
ttttaccaaaggaagacaacaagcagaagaatgcctaactggggtggaggaaaaaaatgtacagtgtgccagaagtctgtttactttgcagaagaggtgc
aatgcgagggaggaagcttccataagtcctgtttcttgtgcatggtatgtaagaagaatttagatagcactacagtagcaattcatggagaagagatata
ctgcaaatcctgttatgggaagaagtatgggcccaaaggctatgggttcggccagggagcaggtactctaagcatggatcgtggagagcacttgggcatc
caaactgatgacccctcaagatcccaaccaacgaataaccccaatgcctccaagtttgcgcagaaagtgggaggtacagacatttgccctcgctgttcaa
aatctgtgtatgctgctganaaagtaattggtgcaggcaattcttggcacaggacctgctttcgctgttctaaatgtggaaaaagattggaatcccacac
agtggctgatagagatggagacatattttgcaaagcgtgctatgcaaagaattttggggcctaaggtttcgggtttggccagggggctggaccgttgggc
ccaccaaagtgatgaccctggaaattctaaaaaaatgaattcccttttccgggcttttaacaattttaccaaacacaaggttggaatattttaccgctgg
cctttccttaactaaatcccacgtaaattatttctccctgggcagtttttaaaaatattacaggcccccgggttttggactatttgtggaaaaccccggt
gggaccccggctggggtattttaaagggtattaattaaaaggaaggctcaccttctcaaatcccgctcaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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