XB-CLONE-4355713
Clone Name: IMAGE:7030242
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Gene:
hnrnpu
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077044 [+]
CCGGTCCTTGCTGGTGCCGCCATTTTGGAAGGAGTAAGAGCGCAACAAACACGCTCGCAAACCCTCTCCTGGTTCAGTCCACTAGCACGCACAGCTGACC
GGCACAATGAGTTCCCCGATAAACGTGAAGAAGCTCAAGGTATCCGAGCTAAAGGAAGAGCTGAAGAAGAGGAACCTTAGCGATAAAGGGCTGAAGGCCG
ATCTGATGGAGAGACTGCAAGCTGCGCTGGACGAGGAGGGAAGCGCTTCTGGAGGAGGGGGAGTGGCGGTCCCTGATGAGGCCGAGGATTTGGGCGATCC
CGAACAAGGTGCAACCGGAGAAGACGAAGAGGAGGAAGAAGAAGAAGGAATGGAGCTAGGAGGAGAAAACGGAGGCGATGGGGCCGATGAGGAGGCGCCC
GACGAGGAGGTACCCGGCGAGGAGGTACCTGAAGCAGAGCAAGGAGATGAGAACGGAGACGATCAGGGTTTCCAGGAGGGAGATGAGGAGGAGGAAGAAG
AGGAAGAAGATGAAGGCATTCCGGTTGGACTGGAGGAAGAAGATGATGCGGAAGCGGCTATCGGAGAGAACGGGCAAGATCAGGAGAAGGAGGAAAGCGA
GAGTGCGCCCAGTGGCCCACAACCACTAATGGCCATAAAGTGCGAAGAGACGGGCGGCCCCGGGAGGAGCGGTGAGTTTATGGAGGATGGAAAAGATCCA
AAAAAAAAAAAAAAA
5' EST: CF782328 [+]taattaatcccccccccccccccggtccttgctggtgccgccattttggaaggagtaagagcgcaacaaacacgctcgcaaaccctctcctggttcagtc
cactagcacgcacagctgaccggcacaatgagttccccgataaacgtgaagaagctcaaggtatccgagctaaaggaagagctgaagaagaggaacctta
gcgataaagggctgaaggccgatctgatggagagactgcaagctgcgctggacgaggagggaagcgcttctggaggagggggagtggcggtccctgatga
ggccgaggatttgggcgatcccgaacaaggtgcaaccggagaagacgaagaggaggaagaagaagaaggaatggagctaggaggagaaaacggaggcgat
ggggccgatgaggaggcgcccgacgaggaggtacccggcgaggaggtacctgaagcagagcaaggagatgagaacggagacgatcagggtttccaggagg
gagatgaggaggaggaagaagaggaagaagatgaaggcnttccggttggactggaggaagaagatgatgcggaaacggctatcggagagaacgggccaga
tcaggagaaggaggaaagcgagagtgcgccccagtggccccccaaccacaaatgggccataaaggtgcgaaaaaaacgggcggcccccgggaaagaaacg
gttgaatttattgggaggaagtggaaaaaaaatcccaaaaaaaaaaaaaaaagagggatcccccttaagttttctagaagggggcccggccccaccccgg
gtggggaaacttttggccccgtgtggcccacaacctttgttttttatttggccgcccctttataaaagggggtttcacaaaaaaaaagggcccatatact
ctcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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