XB-CLONE-4355711
Clone Name: IMAGE:7030235
|
Gene:
decr1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC077043 [+]
AGTTGAATCCTTGCGTTCCAGATGGCGCTGCACTTGTCACCTCTGAGCCGCTGGGCAGTGCTTAGTGCGGGCCGGGTCGGGCAAGGCCACAGGGCATTTA
GCCATGGGACAGCATTATTATCAAGCGAGAACAAAGAACCCCCACAAGCAAAGTTCTTCCCTCCTTTAGAGACGGCAATGTTACCACCCGACTCATTCAA
AGGAAAGGTGGCCTTCATTACAGGGGGTGGGACCGGCCTGGGAAGAGGAATGACCACCACCCCTTCCCGATTAGGAGCCGAATGTGTTATATCCAGCAGA
AAACTGGATGTCTTAAAAAAAACTGCAGCTGAGGTCACATCAGAAACTGGAAACAAGGTTCATCCAGTTCAGTGTGATGTCCGAGATCCCGAGTCAGTTA
AAAAAGCTGTAGAAGAACTGATTCAAGTGGCTGGACATCCAGATGTTGTGATAAACAATGCAGCGGGGAACTTCATTTCTCCTTCTGAAAGACTGTCTGC
AAACGCCTGGAAGACCATCACAGACATTGTTCTTAACGGCACCGCTTACGTAACGCTCGAGGTTGGCAAGGAGCTCATTAAGGCTGGGAAGGGGGCAGCA
TTCCTTTCAATTACCACAGTATATGCCAATAGTGGGTCTGGCTTTGTAGTACCGAGTGCATCTGCCAAGGCTGGAGTTGAAGTGATGTGCAAATCCCTTG
CATCTGAGTGGGGAAAATATGGCATGAGGTTTAATGTGATACAGCCTGGTCCAATAAGAACGAAGGGGGCATTCAGTCGCCTTGACCCCACTGGACAATT
TGAAGCGGAAATGTTGAAAAGGATTCCTTGTGGACGTCTGGGAACAATTGAGGAGATCGCAAATCTTGCCTCCTACCTCTGCAGTGACTATGCTACCTGG
GTCAATGGGGCAATTATTCGTCTGGATGGTGGTGAATATGTATCTATGGCCGGCGAGTTTAATAGTTTAACAAAGGTAACCAATGATCAGTGGGATATGT
TAGAAAAATTAATCAGAAAAAAAAAAAA
5' EST: CF782329 [+]taattaatcccccccccccccagttgaatccttgcgttccagatggcgctgcacttgtcacctctgagccgctgggcagtgcttagtgcgggccgggtcg
ggcaaggccacagggcatttagccatgggacagcattattatcaagcgagaacaaagaacccccacaagcaaagttcttccctcctttagagacggcaat
gttaccacccgactcattcaaaggaaaggtggccttcattacagggggtgggaccggcctgggaagaggaatgaccaccaccccttcccgattaggagcc
gaatgtgttatatccagcagaaaactggatgtcttaaaaaaaactgcagctgaggtcacatcagaaactggaaacaaggttcatccagttcagtgtgatg
tccgagatcccgagtcagttaaaaaagctgtagaagaactgattcaagtggctggacatccagatgttgtgataaacaatgcagcggggaacttcatttc
tccttctgaaagactgtctgcaaacgcctggaagaccatcacagacattgttcttaacggcaccgcttactaaacngctcgaggttggcaaggagctcat
taangggctgggaagggggcagcattcctttcaattaccacagtatatgccaataagtgggtcctggctttgntagtaccgagtgcatctgccaaggctt
ggagtttgaaatgatgtgcaaatccctttgcatctggagtggggaaaaatatgggcatgaagggtttaatgtggataccagcctgggtcaantaaagaaa
cgaaggggggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: