XB-CLONE-4355541
Clone Name: IMAGE:7030089
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Gene:
gtf2a2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077041
[+]
GATATTCATTTCCGGAGCTTGTTGCCGGAAGGGAGAACTTGGAGCATCTGCTATCGTCTTGACAGTATTGGCTTAATTTACAATTACGTCAGTGGATGGT
CGACTGAATTGTCTTCCATTAGCCATGGCTTATCAACTGTACAGGAATACGACACTGGGTAACAGTCTTCAGGAAAGCTTGGATGAGCTAATACAGTCTC
AACAGATCAACCCTCAACTTGCACTTCAGGTTCTGCTACAGTTTGACAAGGCTATTAATTCAGCATTGGCACAGCGGGTCAGAAACAGAGTCAACTTTAG
GGGATCCTTAAACACTTATAGGTTTTGTGACAATGTATGGACCTTTGTGTTGAACGATGTCGAGTTCAGAGAGGTTACTGACCTAGTGAAGGTGGATAAA
GTTAAAATTGTTGCTTGTGATGGAAAAAACACCGGTTCAAACACTGCAGAGTAAGAAAAATGGAATATTTCTCTAAATGGTTTCTGTTGGTCAGCGGCCT
TTCCAGGAATATAACGGACACGACTGTTTATTTATTTCAATGATACAGCCAAAAGAAGCTATCCTACAGCATTCACTCGAGATTTGGAAAATTTCTTTTT
CTACTTACATGTAGCACACAAAAGCTTCCTATATAAGGTTTTTTCTTTTTCTTTTTAATACTAAATGTGAAGTTGCCTTTTTTTTCTTTTTTCAACATGT
CTAATAAACTTTTATTTAACTAAAAAAAAAAAAAAAA
5' EST: CF782414
[+]taattaatcccccccccccccgatattcatttccggagcttgttgccggaagggagaacttggagcatctgctatcgtcttgacagtattggcttaattt
acaattacgtcagtggatggtcgactgaattgtcttccattagccatggcttatcaactgtacaggaatacgacactgggtaacagtcttcaggaaagct
tggatgagctaatacagtctcaacagatcaaccctcaacttgcacttcaggttctgctacagtttgacaaggctattaattcagcattggcacagcgggt
cagaaacagagtcaactttaggggatccttaaacacttataggttttgtgacaatgtatggacctttgtgttgaacgatgtcgagttcagagaggttact
gacctagtgaaggtggataaagttaaaattgttgcttgtgatggaaaaaacaccggttcaaacactgcagagtaagaaaaatggaatatttctctaaatg
gtttctgttggtcagcggcctttccaggaatataacggacacgactgtttatttatttcaatgatacagccaaaaagaagctatcctacagcattcactc
gagatttggaaaaattctttttctacttacatgtagcacacaaaagcttcctaaataagggttttttcctttttcctttttaaaaccaaaatgtggaagt
ttgccctttttttttccttttttttcaacttggtcttaataaaacttttttattttaaccttaaaaaaaaaaaaaaaaaaaaggagtccccctcaagttt
cctaagaagggggggcggcccaccccgggggggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: