XB-CLONE-4355385
Clone Name: IMAGE:7029943
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Gene:
ankrd10
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084530 [+]
GGTGAGAGAGAGCAGACAGTAGTCAGTCATGTCTGTGTGCTCAGAATCCGGCTTCTCCAGCGAGGAGGTGCTCAGTCTGCGGTTCCCTTTACATCGCGCC
TGCCGAGATGGAGACCTGCGCTGTCTCCGCTCACTACTACAGGGCCCTCCGGGAAACACCGCGCTGCAGCTCGGCAAGGAGGACTCCTGCTACGGCTGGA
CCCCCATACATTGGGCCGCGCACTTCGGCAAGCTGGAGTGTCTTATTCAGCTGGTTGGAGCAGGATGCTCAGTAAATGCCTCTACAACTCGGTTTGCACA
AACTCCAGCTCATATAGCAGCTTTCGGAGGGCATCCCCAGTGTTTGATGTGGTTGATTCAAGCAGGTGGCCAAATAAATAAACAGGATTATGTTGGTGAA
ACTCCTGTCCATAAAGCAGCAAGGGCTGGTAGCATGGACTGCCTAAAGGCCCTAATATCCAATGGAGCGCAAATAGACATCAGGAATGCCAGTGGTTTGA
CTGCCGCGGACCTAGCACATTCACAAGGGTTCAGAGATTGCGCTCAGTTCCTCCTAAACGTCCAAAACAGTCAGCTCAACGGTTTCTATTGTAGCAGCTC
CTTAAATGGTGTTCATGAGAGCACCCCATCTAAACACCTCAATGGAATTGCTAATCGCAAAAGGTCTTTCAATGACTTTGAGGCTTCAACTGTCAAGAAA
CCTAGAACAGAAGTGAACGGGTTTGTAGCTGTTAATCCTGAGTTTATGACTGGAGGCTTGGAGGAAGAGGATGAGAGTATGCACGTTGAAACTCACTCAG
CTGAGATATCAGGTGGGATTGGGCAATTTAATGACCACTGCTGCAGCTCTCGAATTCTTGAAGATCTGAAACCCAAGGAGAGAGAGTCTATTAGACCAGC
TGATTCTGATATTTATACGGTGCCTGCCGTGCAAACCCCCTTTCGCTGCATCAGTCAGTATGCTTATTTTTAAAATAGTCAACTGATTGGTGTGCTTTCT
TTTTCTTTCATTGTTTCAAAACGTCGCTACACAAATATATAAAGACTTTGGTAGAATCTGCTTTGCTTGTAACAAGAGAAACCTGGTATTCCATGTACAT
TTTTTTCTCGGAAAAGCTCAAATATACATTGATTCACGCTTTCAATATTATATACAGTATAATAAGGTTATATTGTGTGACTTCTTCTTATTCTTCTCCA
TTGAGCACACTAAGCAAAATATTTACTTGTATTTTTTTTTTTTTTTTAAATAAAGTGTAATATTAAATGCAAATTAAATTTGATGTTCTCTAATGGGAAA
AAAAAAAAAAAAA
5' EST: CF782492 [+]taattaatcccccccccccccggtgagagagagcagacagtagtcagtcatgtctgtgtgctcagaatccggcttctccagcgaggaggtgctcagtctg
cggttccctttacatcgcgcctgccgagatggagacctgcgctgtctccgctcactactacagggccctccgggaaacaccgcgctgcagctcggcaagg
aggactcctgctacggctggacccccatacattgggccgcgcacttcggcaagctggagtgtcttattcagctggttggagcaggatgctcagtaaatgg
cctctacaactcnggtttgcacaaactccagctcatatagcagctttcggaaggcatccccagtgtttgatgtggttgaattcagcaggtgggcaaataa
taaacaggaatatgttggtgaaacttctggtccataagccaccaagggctggtaacatggatggctaaaggcccttaattccatgggaccccaataaaac
tccggaatgccccgggtttaaatgcccggaaacttaaaatttccaagggggtcaaaaaaatggggcccattttcccccccaaaaggcccaaaaaaaaggg
gcccccccggggtttttttttttggaaaaccccccccctaaaaagggggggttccaaaaaaaaaaaccccccccttataaaaaaccccccgcgggggggg
gattgtggttcccctccccccaaagggggggggttttttttaaaggagaggatttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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