XB-CLONE-4355363
Clone Name: IMAGE:7029909
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Gene:
fus
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077686 [+]
ACAGTGCGCAGCGGAGGTGATATATCGTGGTTACTAGGCGGTACTTGGAGAACATCAGTGATACCGCAACATGGCCACCAACGATTACTCGCAACAAGCC
AGCCAAGGGTATGGGGCATACCCCTCCCAACCAAGCCAGGGTTATGGGCAGCAGGGCAGCCAGTCCTACAGCCAGCAAGGTTACAGTGGTTACGGCCAGT
CTGCAGGTGGCTCCTCGTATGGACAGAGTGGCTACAGCTCTTCATGTGGTCAAGACCAGAGTGCTGGATATAGTTCTCAGTCAACTCCACAAGGATATGG
ATCTGGTGGTTATGGAAACTCCCAATCATCTCAGTCCCCTTATGGCAGCGGGCAGCCACAGCAGCAGTCCCAGTCCTCTTACTCCAGCTACTCCCAGCAG
CCTCCAGCCAGTAGCAACACAGGAAGTGGCAGCTCCCAGCCATCCAGCTACCAGCAGCAGCAGAGTGGTGGATATGGGCAGCAACCAAGTGGGGGTTATG
GAAGTCAGCAGAGCAGCTATGGAGGTCAACAGCAATCCAGTTATGGGAGCCAGCAGGGTCAGAGTGGCTATGGCAGTCAGCAGCCAAGCTCTTATGGACA
GCAGCAGCAATCAAGCTACAGCCAGCCCCAGGGCTATGGCCAGCAGCAAACACAGTATAGTGGTGGAGCTGGTGGATATAATCAGGACTCTCCTTCCATG
GGCAGCGGAAGCTATGGTGGAGGGTCTGATCAGGGTGGCTATGGAGGCCAAGATAGAGGAAGGGGACGTGGAGGATTTGGTGGTCGTGGTGGTGGTGGTG
GAGGAGGAGCAGGTGGCTTTGACAGCCGCGGAAGGGGCAGCCGTGGTGGCAGAGGCGGCATGGGGTAGGTATTTCATCAGGCAAAGTTAGAAATTACAGC
TGTGAGTGTGTGTAATGTGTGAGAAAGTGGTCTGTGTTGGAAGCAATTTGAGTGAATTCTTGTTCCTTCTGGATTTTGATGTGTAGCCTAAAAAAAAAAA
AAAAA
5' EST: CF782503 [+]taattaatcccccccccccccacagtgcgcagcggaggtgatatatcgtggttactaggcggtacttggagaacatcagtgataccgcaacatggccacc
aacgattactcgcaacaagccagccaagggtatggggcatacccctcccaaccaagccagggttatgggcagcagggcagccagtcctacagccagcaag
gttacagtggttacggccagtctgcaggtggctcctcgtatggacagagtggctacagctcttcatgtggtcaagaccagagtgctggatatagttctca
gtcaactccacaaggatatggatctggtggttatggaaactcccaatcatctcagtccccttatggcagccggcagccacagcagcagtcccagtcctct
tactccagctactcccagcagcctccagccagtagcaacacaggaagtggcagctcccagccatccagctaccagcagcagcagagtggtggatatgggc
agcaaccaagtgggggttatggaagtcagcagagcagctatggaggtcaacagcaatccagttatgggagccagcagggtcagagtggctatggcagtca
gcagccaagctcttatggacagcagcagcaatcaagctacagccagcccccagggctatggccagcagcaaacacagtatagtggtgggacctggtggga
tataatcagggacttctccttccagggggcaggcgggaaacctatgggggggaggggccttgaatcagggggggcctaatgggagggcccaagaatagag
ga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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