XB-CLONE-4355159
Clone Name: IMAGE:7030545
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Gene:
myl9
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077710 [+]
AGTGCAGAGCTTCCCCGGCTCCTGAGCAGCCACACTGAGCGGCACTCACACTCATAGCAGGTACCATAACCACACGCAACCATGTCCAGCAAGAAAACCA
AAGCAAAGACCAGCAAGAAGCGTCCACAGAGGGCGACTTCCAATGTGTTCGCTATGTTCGACCAGTCCCAGATACAGGAGTTTAAGGAAGCCTTCAACAT
GATTGACCAGAATCGAGATGGCTTTATTGACAAGGAAGATCTACATGATATGCTGGCCTCTATGGGTAAAAACCCCACTGATGAATATCTGGAAGGAATG
ATGAGCGAAGCCCCAGGACCCATCAACTTCACTATGTTCCTAACAATGTTTGGAGAGAAACTGAACGGCACAGACCCCGAGGATGTCATCAGGAACGCCT
TTGCCTGCTTTGATGAGGAGGCGTCAGGATTTATCAATGAAGACCACTTGCGAGAGCTCCTCACTACGATGGGAGACCGCTTCACAGACGAGGAGGTGGA
TGAGATGTATAGAGAGGCCCCAATTGACAAGAAAGGCAACTTCAACTATGTGGAATTTACCCGTATCCTGAAACACGGGGCCAAGGAAAAAGACGACTAG
AGTTACGACAAGACACCGATGCCTTGTGGCCCTAAATGAAGGAGATGCTGTGCAAGCAATGGGCAGCGCTTATGTCTCCCAGTGTGTAATTATTTTATAT
GAGAAGCCTCCCCATGCAAAGTCAACCGTGGCAGCGGCCATATTTAAAATCCTCCACAAAAAAATGACTGTAATGCGCCTACTCTTCACCCCTGTCACCT
TAGCCGTTCCAATGGGAAGACATCATATTAATATATAAATAAATCAATTTATTCGTGAAAAAAAAAAAAAAAA
5' EST: CF782605 [+]taattaatccccccccccccgagtgcagagcttccccggctcctgagcagccacactgagcggcactcacactcatagcaggtaccataaccacacgcaa
ccatgtccagcaagaaaaccaaagcaaagaccagcaagaagcgtccacagagggcgacttccaatgtgttcgctatgttcgaccagtcccagatacagga
gtttaaggaagccttcaacatgattgaccagaatcgagatggctttattgacaaggaagatctacatgatatgctggcctctatgggtaaaaaccccact
gatgaatatctggaaggaatgatgagcgaagccccaggacccatcaacttcactatgttcctaacaatgtttggagagaaactgaacggcacagaccccg
aggatgtcatcaggaacgcctttgcctgctttgatgaggaggcgtcaggatttatcaatgaagaccacttgcgagagctcctcactacgatgggagaccg
cttcacagacgaggaggtggatgagatgtatagagaggccccaattgacaagaaaggcaacttcaactatgtggaatttacccgtatcctgaaacacggg
gccaaggaaaaagacgactagagttacgacaagacaccgatgccttgtggccctaaatgaaggagatgctgtgcaagcaatgggcagcgcttatgtctcc
cagtgtgtaattattttatatgagaagcctccccatgcaaagtcaaccgtggcagcgggcatatttaaaatccctcacaaaaaaatgactggtatgcgcc
tactcttcacccctgtcactttagccgtttccatggggagacatcttattaatatac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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