XB-CLONE-4355053
Clone Name: IMAGE:7030482
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Gene:
akr1b1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084532
[+]
GCTGTCAGTGTTAGAAGTCTCAGCTCTCAGCAGCGGCCGCAGGGATGAAGGGCCCGACCCATGTGCAGCTCTATACCGGGGCCCAGATGCCCATTGTAGG
TCTGGGAACATGGAAGTCTGAGCCGGGGAAGGTGACGGCGGCGGTGGCCAAGGCTATTGAGGTGGGCTACAGACACCTGGACTGCGCCTATGTGTATCAG
AATGAGAATGAGGTGGGAGAGGGGATCCAGCAGAAGATTAAGGAGGGACTGGTGAAGAGAGAAGACCTGTTCGTCGTTAGCAAGCTGTGGAGCACCTTCC
ATGACAAATCCATGGTGAAGGGGGCCTGCCAGAAAACCCTGTCCGACCTGAAACTGGATTACCTGGACCTCTACCTGGTCCATTGGCCGACAGGGTTCCA
GGCTGGCGATGCGCTCTTCCCACTCGACAACGAGGGCTGCGTAATTCACAGTAATACGCATTTCCTGGATACCTGGGAGGGGATGGAGGAGTTGGTAGAC
GCCGGGCTAGTCAAGGCCATCGGCATCTCGAACTTCAACCGCGAGCAAATAGAGCAGCTGCTGAATAAACCAGGATTGAAGCACAAGCCGGCGGTTCACC
AGTTTGAATGCCACCCCTACCTGACCCAGAAGAAACTGATTGATCTCTGCCAGTCCAAGGGGATCGTGGTCACTGCCTACAGCCCCCTGGGCTCCCCAGA
CCGGCCCTGGGCCAAACCGGAAGATCCGTCTCTGCTCGAGGAGCCCAAAATCAAAGAAATTGCTAAAAAGTACAACAAGACCTCGGCCCAGGTTCTGATC
CGGTTCCATATCCAGCGAAATGTTGTCGTCATCCCCAAATCTGTCACCCCGGCGCGCATTGAGGAGAACTTCCAGGTGTTTGACTTTGAGCTGTCTCCGG
AAGACACGGAAGCCATCTTTAGCTTCGAGCGCGGCTGGCGAGTGTGCGCGCTTTCCTCGGCCAAGAAGCACAAGGATTACCCCTTCCACGCAGCGTATTA
GGCAACTCGCCGGCTGTTTCACCTCCTACTTTTCTCATTGGTCGTGTTTTGTTACAAGGTTATCGCTGGGGTGGACGGGGTTGAAGCTTCATATTCTTGG
GCTGGGGGGCAAATTCCATGGCCTTGATGTTATTAAACTGCCGCTGCTCCACAGTTACCCACATAAAGGAGTTGGTTACCTTTAAATAAACCTTTAGATG
TTCAGAGACAGTTTGCAATTGGTCTTCATCTTTTATATTTTGCAGTTATTCATTTTAGTTTTTCTGTTTCTCCAGTAGCTACGTGGGTGCTAGGGGTCTG
GAATATGAATAGGAAAGATAAGTAACAAAAACTAAGAATACATTTTTTTTTTTTTAAAAAATGAAGACCAATTGCAAAGTTGTAGGAGTGGGACAGTCTG
TAACATACTCAAAGGTAACGGAGGGGAACTACTTCTTAGAACCAGTTGAAAGTCACATTGTGCCTTGTCCAGGTAGGAGATATAGCGGCACCTTTACAGC
CCCAATACTGGGGCCTATGGATCACTCCATTCTGTACTGACTGTAACTGCCCCCTGCCCCGTGTACTGTAACTGCCCCGTAGGCAAAGCTGTGCTTGTTC
TATTAAAAAAAAAAAATAAAAGCCCAAAAAAAAAAAAAAAA
5' EST: CF782658
[+]taattaatcccccccccccccgctgtcagtgttagaagtctcagctctcagcagcggccgcagggatgaagggcccgacccatgtgcagctctataccgg
ggcccagatgcccattgtaggtctgggaacatggaagtctgagccggggaaggtgacggcggcggtggccaaggctattgaggtgggctacagacacctg
gactgcgcctatgtgtatcagaatgagaatgaggtgggagaggggatccagcagaagattaaggagggactggtgaagagagaagacctgttcgtcgtta
gcaagctgtggagcaccttccatgacaaatccatggtgaagggggcctgccagaaaaccctgtccgacctgaaactggattacctggacctctacctggt
ccattggccgacagggttccaggctggcgatgcgctcttcccactcgacaacgagggctgcgtaattcacagtaatacgcatttcctggatacctgggag
gggatggaggagttggtagacgccgggctagtcaaggccatcggcatctcgaacttcaaccgcgagcaaatagagcagctgctgaataaaccaaggattg
aagcacaagccgggcggtttccccagtttgaaatgccaccccctacctggacccaagaaaaaaacttgatttggatctcctgccaagttcccaaggggga
attcgtggttccactgtccttacaggcccccccgggggcttcccccagaaccgggccccttggggcccaaaacccgggaaaaaatacccgttcttctggc
ctttcgaaggaagcccccaaaaaatccaaaagagaaaatttgggtcttaaanaaaagggttccaaaaccaagaggaaccctctcccggggcccca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: