XB-CLONE-4355029
Clone Name: IMAGE:7030469
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Gene:
smarca4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084531
[+]
GAGAGAAATTGAGCCGAGGTAGCGGCGGGAACGACCCGGTGTTCCCGGTGATAGTTGGAGAGAGACGGCTGAAGGATTTGGGGACAAGTTAGGTTTGCAG
TGATTGGATCTTCTCCCTGCAGTGATGTCCACCCCAGACCCTCCAATGGGGGGCACTCCCCGGCCTGTACCTTCCCCCGGGCCAGGGCCTTCTCCTGGAG
CTATGCTAGGCCCCAGCCCAGGCCCCTCCCCTGGCTCTGCACACAGCATGATGGGACCAAGCCCCGGGCCACCTATTCCTCCACAGGGACCTGCTGGGTA
CCCACCTGAAAATATGCACCAGATGCACAAGCCAATGGATTCCATTCATGACAAAGCAATCCCTGAAGACCCCCGATACAATCAGATGAAGGGTATGAGC
ATGAGACCTGCTGCTCACTCTGGGATGGGGCCTCCACCCAGTCCTATGGACCAGCACTCTCAAGGATACCCATCACCTCTGGGCAACTCTGAGCACGTGC
CCAGCCCTGTACCATCTAATGGTCCTCCCTCTGGCCCTCCGATGCCTTCTGGGCCATCAGCGGTGAATATGGAGAACTCGGACCCACAACAGCAGACAAG
CCGAAGTCCTACTCCCTTCAACCAGAACCAGCTGCACCAGTTGCGGGCTCAGATAATGGCTTACAAAATGTTAGCCAGGGGGCAAGCTTTACCCGACCAC
CTTCAGATGGCAGTGCAGGGAAAGAGACCCATGCCTGGGATGCAACAACAGATGCCAACACTACCTCCACCTGCTGCTTCGGGGACTGGCGCTGGTCCTG
CCCCAGGCCCAGTGCCCCCTAACTACAACAGACCTCATGGAATGGCTGGTCCTACTATGCCACCTCCCGGCCCTTCAGGAGCTCCACCTGGAATGCCTGG
CCACCCTGCTGGTGCTCCACCTAAGCCTTGGCCTGAAGGTCCCATGGCTAATGCAGCTGCCCCTGCCACTGCCCCCCAGAAGCTGATTCCTCCACAGCCT
ACTGGCCGCCCTTCCCCTGCCCCTCCGGCTGTCCCACCGGCTGCTTCCCCCGTGATGCCTCCGCAGACACAGTCTCCAGGCCAGCCGGCTCAGCCTCCTC
CCATAGTACAGTTTCACCCTAAGCCCAACCGCATCACTCCCATACAGAAACCGCGAGGCCTGGATCCTGTGGAGGTGCTACAGGAACGGGAGTACAGGCT
GCAGGCGAGAATTGCACACAGGATCCAGGAACTGGAAAATCTTCCTGGTTCTTTGCCTGGAGATCTAAGGACCAAAGCCACCATAGAGCTGAAAGCTTTA
AGGCTTCTCAATTTTCAGAGACAACTGCGGCAAGAAGTTGTGGTCTGCATGCGGAGGGACACGGCCCTTGAGACCGCCCTAAACGCAAAGGCCTACAAAC
GCAGCAAGCGGCAGTCCCTGCGCGAGGCGCGTATCACGGAAAAACTGGAAAAGCAGCAGAAGATTGAGCAGGAGCGCAAGAGGAGACAAAAGCACCAGGA
GTATCTAAACAGCATCCTCCAACATGCCAAGGACTTCAAGGAATTCCATCGCTCAGTCACAGGGAAGATCCAAAAACTGACAAAGGCTGTGGCCACGTAC
CATGCCAACACCGAGCGAGAGCAGAAGAAGGAGAATGAAAGGATTGAGAAGGAGAGAATGCGCAGGCTCATGGCTGAGGATGAGGAGGGCTACAGAAAGC
TGATCGATCAGAAGAAAGACAAGCGTCTGGCCTACCTCCTGCAGCAGACGGACGAGTATGTAGCAAATCTGACGGAACTGGTGCGGCAGCACAAGGCAGT
GCAAGCCCTCAAAGAAAAGAAGAGAAGGAAAAAAAAAAAAAAAA
5' EST: CF782670
[+]taattaatcccccccccccccgagagaaattgagccgaggtagcggcgggaacgacccggtgttcccggtgatagttggagagagacggctgaaggattt
ggggacaagttaggtttgcagtgattggatcttctccctgcagtgatgtccaccccagaccctccaatggggggcactccccggcctgtaccttcccccg
ggccagggccttctcctggagctatgctaggccccagcccaggcccctcccctggctctgcacacagcatgatgggaccaagccccgggccacctattcc
tccacagggacctgctgggtacccacctgaaaatatgcaccagatgcacaagccaatggattccattcatgacaaagcaatccctgaagacccccgatac
aatcagatgaagggtatgagcatgagacctgctgctcactctgggatggggcctccacccagtcctatggaccagcactctcaaggatacccatcacctc
tgggcaactctgagcacgtgcccagccctgtaccatctaatggtcctccctctggccctccgatgccttctgggccatcagcggtgaaatatggagaact
cggacccacaacagcagaacaagccgaagtcctactcccttcaaccagaaccagctgcaaccagttgcggggctcagataatggcttacaaaatgttagc
caggggggcaagctttacccgaccactttcaaatggcanttgcagggaaagaagacccatgcctggggatgcaacaacagatggccacactacctccact
ggcgggcttcggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: