XB-CLONE-4355007
Clone Name: IMAGE:7030458
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Gene:
bub1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077709 [+]
AAGCAGAAGCAGGATGGGACGTGCGTGATGTTGTAGCGGAATAGCGGGGCCCTGGAATCGCCACCTTACTGCTCATCATAAAAATGGATCTACAGAGCCA
TGCACAGATGTTTGAAGCCCATATTCAGGGATACAAAGGCGATGATCCACTTGATCTTTGGGAAAGGTATGTACTTTGGGCAGAAGAAACTCTCCCCCCA
CAAGAGAAGCAGAACATTTTTTGTTTGCTTGAGCGTCTAGTTAGAAACTTCATAGGAGACAAAAGATACAGCAATGATGAGCGATATCTGAAGTACTGCA
TCAGATTTGCTGAAACTATCAATGAGCCAGCACAATACTTTGAGTACCTATATAACCAAGGCATCGGCCATCAGTCTGCCCCTCTGTACGTGACATGGGC
TCAGTTGCTAGAGACACAAGGAGACCTGCAAGGTGCCAGTACTCTGTATCAGAAGGCAATTCACAGCCATGCAAAACCTAAAGAAATCCTGGACCAGCAC
TTCAGAGCATTCCAGATCAGGGTCTCTCAAGCAAATATGCAAAATCATGGTGCCCTTGTGGAACCCCTCAGGAATTCTCAGATTTTAAATCAAATGAACT
CTACTTCATCAAATGTGGATCAATTATCTCTTGCCAAATGTGAGAGTTCTGCTCCTTCACAAATTCACACTTCTCAAGAATCTGCATGTGATGTTGATAG
AAGTGGGAATAAATGGGTCACCATCTCCAAGTCAGCTGTGGTTCCACAGCCTGCTAAATCTGTGGGTGTGGAAGTTAAACAGGTTCCTATGTACTGTAAG
GATAAGCTGGTCTGTTCCGATTCTGAGCTTTCGTTTGAGGAATTCCGAGCAAATATATACAAAAAAAAAAAAAAAA
5' EST: CF782681 [+]taattaatccccccccccccgaagcagaagcaggatgggacgtgcgtgatgttgtagcggaatagcggggccctggaatcgccaccttactgctcatcat
aaaaatggatctacagagccatgcacagatgtttgaagcccatattcagggatacaaaggcgatgatccacttgatctttgggaaaggtatgtactttgg
gcagaagaaactctccccccacaagagaagcagaacattttttgtttgcttgagcgtctagttagaaacttcataggagacaaaagatacagcaatgatg
agcgatatctgaagtactgcatcagatttgctgaaactatcaatgagccagcacaatactttgagtacctatataaccaaggcatcggccatcagtctgc
ccctctgtacgtgacatgggctcagttgctagagacacaaggagacctgcaaggtgccagtactctgtatcagaaggcaattcacagccatgcaaaacct
aaagaaatcctggaccagcacttcagagcattccagatcagggtctctcaagcaaatatgcaaaatcatggtgcccttgtggaacccctcaggaattctc
agattttaaatcaaatgaactctacttcatcaaatgtggatcaattatctcttggccaaatgtgagagtttctgctccttcacaatttcacacttttctc
aagaatcttgcatggtgatgtttgaaaagaagttggggaataaaatggggtccaccattctttccaagtccaggctgttgggtttccaccgacccctgcc
ttaaaattctggtgggggtggttgggaaaatttaaaaacaagaggttttccccttagtgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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