XB-CLONE-4354887
Clone Name: IMAGE:7030391
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Gene:
snrnp27
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077690 [+]
AGGTTCCAGGGCGGAACCTTTCGCAAGCGGAAGTTGTTTCGGTCGGAAGGCTCTTGTTGCTCGCGTGTAGAAGTTTCTGGCAGGGGTAGTTCCGGTGTGT
ACGGAAGGGATACAAAATGGGTCGCAGCCGGAGTAGATCCCCTGAAAGGCGGCGAGAACGCAGGCGTTCTCGATCCGCTTCACGGGAGCGAGAAAGGAGG
AGGAGAGAGCGCTCTCGATCCCGAGAAAGACGTAGGAGCCGCTCACGATCCCCACACAGACGCCGCTCTAGGTCTCCCCGCCGTCACAGGTCTTCCTCCA
TTTCTCCTGGTAGGCTGAAGGACAGACGTGATGATGACAAGAAAGATTCTAAGGAATCTAAAGGTGCAAAGGAACGTCAGATAGCAGCGGAAGATCTTGA
AGGGAAGACAGAAGAGGAAATTGAAATGATGAAGATGATGGGCTTTGCTACATTTGATACCTCCAAGGGAAAGAAAGTAGATGGATCCGTTAATGCATAT
GCCATAAATGTTTCTCAAAAGCGAAAATACAGGCAGTACATGAACAGAAAAGGAGGATTTAACAGACCCCTGGATTTCATTGCTTAAGGTGTAAGACCGT
CATGGTTCCAGTTACACAAGATGTACAGTATGTAAACTCAGAGATTGAAAGGTGTCATGGCCCACAAACTGAGCTGCATTTCACAGCCAGCAAAGCATTC
TTATTGGAAGTTTATTAGCATGTGTTTGTGTGTGGCCCAGCGCATCCCATCCTAGAAAGCATTTCCTGGCTTTCACACTGCTGAAGAAAGCACAGCTTGG
AAAATAGAGCCCTTTGTGTTTTTTTGCTCCTAAGGCTTGGACTAGTTGCCCCCCATACACAGTTAGGCATGGTATTAGTTTGGTGATACATATGAGACTA
GTTGGAGTGTCCCCACCCTAATCACTCATGTTTTGTGTTTTATTACTGTCAGAAATAATGTGGTGTAAATAAACTGTGACTGATCTGAATCTTTTTATAA
AATGTGTGAGACAAAAAAAAAAAAAAAA
5' EST: CF782741 [+]taattaatcccccccccccccaggttccagggcggaacctttcgcaagcggaagttgtttcggtcggaaggctcttgttgctcgcgtgtagaagtttctg
gcaggggtagttccggtgtgtacggaagggatacaaaatgggtcgcagccggagtagatcccctgaaaggcggcgagaacgcaggcgttctcgatccgct
tcacgggagcgagaaaggaggaggagagagcgctctcgatcccgagaaagacgtaggagccgctcacgatccccacacagacgccgctctaggtctcccc
gccgtcacaggtcttcctccatttctcctggtaggctgaaggacagacgtgatgatgacaagaaagattctaaggaatctaaaggtgcaaaggaacgtca
gatagcagcggaagatcttgaagggaagacagaagaggaaattgaaatgatgaagatgatgggctttgctacatttgatacctccaagggaaagaaagta
gatggatccgttaatgcatatgccataaatgtttctcaaaagcgaaaatacaggcagtacatgaacagaaaaggaggatttaacagacccctggatttca
ttgcttaaggtgtaagaccgtcatggttccagttacacaagatgtaccgtatgtaaactcacagattgaaaaggtgtcatggccccacaactgaactgca
tttcacagccagctaagcatttcttattggaagtttattaaccaggggtttggggtgtgggcccaaacccatcccaatcctaaaaaagcatttccatggc
ttttcacactggctggat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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