XB-CLONE-4354861
Clone Name: IMAGE:7030375
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Gene:
ighmbp2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082748 [+]
AAGGCCGAGTGTTTGCGCTACCGGGCAGTAAGGGCTCATTGCCTACACTGTGCCCGGTCAGAGCGATATGGAGGAATTTGTGGAGAAGCAGCTGGAGCTC
CTGAGGGAAGAGAGAGAGGCAGAGATCCAAGAGACAAGAACATGGCGGGAAAACATCTCTTTAAAAGAACTGCAACGTAGAGGAGTGTGCCTTTTGAAGC
TGCAGGTAGCCAACCAAAGGACAGGGTTGTACGGACGGCTGCTTGTCACTTTTACACCCAGGAAATATGACTCTGAAGCAATTCTCCCAAGTAACAGCTT
TGGCCCAGGGGATATTGTGGGACTGTATGAAGCAAGTAGCCCAAATGAACAGCTTGCTACAGGGATTGTGGCCGGTGTTACACAAAAGGTTGTTAATGTG
GCTTTTGATGAATCCAGCAATGATTCGTTAAATCTGGGGACCGAGTGTACCTACCGACTCCTGAAATTAGCCAACGATGTGACCTACAAAAGAATAAAAA
GAGCTTTGACCGCATTGGGCCAGTACCGCAGTGGACAAGCATCTAGTCTCATTGATGTCCTCTTCTGCTCATCTGAACCCAGTCACTCCGTGCAAAAAAG
ACAGGTGGAGTTTTTCAATTTGTCCTTGGATGATTCACAGAAAGAAGCTGTGAGTTTTTCTCTCAATCAGAGAGAGGTTGCTGTTATTCATGGTCCACCG
GGTACGGGAAAAACCACCACAGTGGTAGAAATTATTCTTCAAGCTGTGAAGGAAGGACTGAAGGTCCTCTGCTGTGCCCCTTCAAATGTTGCTGTAGACA
ATCTGGTGGAACGCTTGTCTGTTTTTAAAGAGAAGATCTTGCGTCTGGGTCACCCAGCTCGTCTGCTGGAGTCCTTACAGCATCACTCCCTGGATGCCGT
GCTGGCACGAAGCGACAATGCACAGATTGTGGCTGACATAAGGAGAGATATTGACCAAACCTTTGCTAAAATGAAAAAGCTGCACGGCAAGCAAGAGAAA
AGCGGGTACAGAACGGAAATTAAAGCTCTAAGAAAAGAACTGAAAGAGAGAGAAGAAGCTGCCATGGTGGAGACATTAAAAAGGGCCAATGTTATCCTTG
CGACAAACACAGGTGCTTCACTGGACGGCCCGCTGAAATTGCTTCCGGAAGATTATTTTGACCTAGTGGTGATTGATGAGTGTGCCCAAGCATTAGAAGC
AAGCTGCTGGATATCCCTACTGCAGGCTAAGAAGTGTATTTTGGCTGGAGATCATAAGCAACTACCTCCTACTATAATCTCGCACAAAGCCGCAGCCAAT
GGCTTGTCTCTTAGCCTGATGGAGCGCGTAACTGAGAAGTATGGGGAGAAAGTTGTGAAAATGCTGACCGTTCAGTACCGTATGCATCAGTCTATAATGC
AGTGGGCCTCAGAAGCACTGTACGGAGGGCGACTTACTGCTCATTCCTCAGTTTCACAACATTTATTGAAGGTCGTTGTCCTGCTGGAAGGCAAACCTCC
GCCTCAGTCTCAAGTCTTTTGCAAACTCCAAGAGATTTTCCTCCAAGATTGCCGTGTATTTGGCTCCATACATCTTCCCATCAACTCTGACCAGCTTCCC
TGTCCCTGCTGAAGAGAAGCCCCCCCAGAGCATGATGCTGCCACCACCATATTTGACAGTGGGGATGGTGTGTTCAGAGTGATGTGCAGTGTTAGTTTTC
CGCCACACATAGCGTTTTGCATTTTGGCCAAAAAGTTCCATTTTGGTCTCATCTGACCAGAGCACCTTCTTCCACATGTTTGCTGTGTCCCCCACATGGC
TTGTGGCAAACTGCAAACGGGACTTCTGTTAACAATGGCTTTCTTCTTGCCACTCTTCCATAAAGGCCAACTTTGTGCAGTGCACGACTAATAGTTGTCC
TATGGACAGATTCCCCCACCTGAGCTGTAGATCTCTGCAGCTCCCCCAGAGTCACCATGGGCCTCTTGGCTGCATTTCTGATCAGCGCTCTCCTTGTTTA
GGTGGACGGCCTTGTCTTGGTAGGTTTACAGTTGTGCCATACTCCTTCCATTTCTGAATGATCGCTTGAACAGTGCTCCGTGGGATGTTCAAGGCTTTGG
AAATCTTTTTGTAGCCTAAGCCTGCTTTAAATGTCTCAATAACTTTATCCCTGACCTGTCTGGTGTGTTCTTTGGACTTCATGGTGTTGTTGCTCCCAAT
ATTCGCTTAAACAACCTCTGAGGCCGTCACAGAGCAGCTGTATTTGTACTGACATTAGATTACACACAGGTGCACTCTATTTAGTCATTAGCACTCATCA
GGCAATGTCTATGGGCAACTGACTGCACTCAGACCAAAGGGGGCTGAATAATTACACACACCCCACTTTGCAGTTATTTATTTGTAAAAAATGTTTGGAA
TCATGTATGATTTTCGTTCCACTTCTCACGTGTACATCACTTTGTATTGGTCTTTCACGTGGAATTCCAATAAAATTGATTCATGTTAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAA
5' EST: CF782754 [+]taattaatccccccccccccaaggccgagtgtttgcgctaccgggcagtaagggctcattgcctacactgtgcccggtcagagcgatatggaggaatttg
tggagaagcagctggagctcctgagggaagagagagaggcagagatccaagagacaagaacatggcgggaaaacatctctttaaaagaactgcaacgtag
aggagtgtgccttttgaagctgcaggtagccaaccaaaggacagggttgtacggacggctgcttgtcacttttacacccaggaaatatgactctgaagca
attctcccaagtaacagctttggcccaggggatattgtgggactgtatgaagcaagtagcccaaatgaacagcttgctacagggattgtggccggtgtta
cacaaaaggttgttaatgtggcttttgatgaatccagcaatgattcgttaaatctggggaccgagtgtacctaccgactcctgaaattagccaacgatgt
gacctacaaaagaataaaaagagctttgaccgcattgggccagtaccgcagtggacaagcatctagtctcattgatgtcctcttctgctcatctgaaccc
agtcactccgtgcaaaaaagacaggtggagtttttcaatttgtccttggatgattcacagaaagaagctgtgagtttttctctcaatcananagaggttg
ctgttattcatggtcccaccggggtacgggaaaaaccaccacagtgggtagaaattattcttcaagcctgtgaaggnaaaggactgaaggtcctctgctg
tgcccctttcaaatgttgctg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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