XB-CLONE-4354825
Clone Name: IMAGE:7030351
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Gene:
nck2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080494 [+]
GTGTGTAGGACCGGAGCCTGCAGTAACAAGCTCTTCGGGAGATATTGACTCCATGAAAGATGACAGAGGAAGTCATTGTTATTGCCAAGTGGGATTATAC
GGCACAACAGGACCAAGAACTTGATATTAGAAAAAATGAACGCCTGTGGCTACTTGATGACTCCAAAACCTGGTGGAGGGTAAGAAACACTGCCAACAAA
ACTGGATACGTGCCATCAAACTACGTGGAGAGGAAGAACAGCTTAAAAAAAGGATCTCTTGTTAAAAATTTAAAGGACACTCTGGGTCTTGGCAAAACAA
AAAGCAAAAAAAATAGCACTCGAGATGCCTCACCCACACCAAGCACTGATGCGGAATATCCATCTAATGGCAGCAATACAGATCGAATCTATGATTTAAA
CATTCCTGCAGTAGTCAAATTTGCCTATGTAGCAGAGAGAGATGATGAGCTGACACTCGTGAAAGGCACACGAGTGGTTGTTATGGAAAAGTGCAGTGAT
GGATGGTGGCGTGGTGCGTACAATGGACATGTTGGCTGGTTTCCATCTAACTATGTAGTAGAAGAAGCTGATGACACAACTCCAGATTCCCCAAGTTTTC
TAAGCTCAAGGAAAGGAGCTTCTATGACCAATGGGCAAAATACAAAAGTGCTTCACATTGTTCAGACATTGTACCCATTCAGCTCAGTAACTGAAGAGGA
GCTGAACTTTGAGAAAGGTGAAACTATGGAAGTAATTGAAAAACCAGAGAATGACCCTGAGTGGTGGAAATGTAAAAACTCTAGTGGGCAAATTGGACTT
GTACCCAAAAACTACGTTGTTATTTTAACTGATGGCCCTTCTATGAACAGTTCCCATATTACTCAGATAAGCTACACAGGTCCTGCGTGCACCGGCCGGT
TTGCCGGTAAAGCATGGTATTATGGTGGTATAACCCGACATCAAGCGGAATGTGCCCTGAATGAAAGAGGAATGGAGGGAGACTTTGTTATTAGAGACAG
TGAGTCTTCGCCCAGTGACTTTTCCATATCTTTGAAGGCATCAGGAAAAAATAAACATTTTAAAGTACAGCTGGTGGACAGTGTATACTGCATTGGACAG
CGGCGATTTAAGAACTTAGATGAATTGGTGGAACATTATAAAAAAGCCCCTATCTTCACTAGTGAACACGGAGAAAAGTTGTACCTTGTTAAACCACTAC
AGTGACAACCCCGGAGACCAAGCTACCCCTACCGGGTTCTCCCAACTTCAAGCCTTAGTTCTGACAAATAACTTCCATGGAACAGAGCCATCATTCCAAA
AGAAGCAAATGAGCACTCTTAAAATAATGGCTCACCTTATACCACAAAACACTCTTTGCATTGTACTTTTATTTTTCTGTTGTGTGTTTGTCCTTTCTGT
TTGTGTGTCACATTGCCCTCAGGTTAAACACCTCACTTAAGTGACCATTGTAAGCACTCACTGTCATGCCTTGGTTTAGACTCCTATTCTGCATATGTTT
ACAGTATAATGCTGGTCCTGGCTAGCAAATGGGTAGGGGTCAACTTGAACAGTGTTAATGTACTTAAATCCTTTTTTTTTTATAATATAAGAATTCTTTA
TTGGAAATAGACACAGATAAAATGCACTGAAGCTGCAGCAAATAATTATTGTAGACATGCTGTATCAGTTGTGGCTGTGAATTCTGTAGCCTCATTGCTT
TACCAAGTAGTACATTCAAAAATGTCATAGATCCTAGGCACTCTTTACACTGTATAGTACTCTGTGTCTGCATTACCTCTTGACCACTTTGCAGAGTATA
CTATGTAAAGATATTGTAGCAGTAATTCTTCAACTACTTTGGTACAATTGATTGTAGTATATAGTGTATTTTGATATGTTATTACTGCAAAATAATTACT
GAATGACTTTGATTCCAAGTTTTGCATAAAAGTTAACCAAATGTGCCATCCACATGTTATTGTTTTTTTTCTTGCCCTTCTGCTCGTTGAAATTGAACAG
TTATGTATTTAATTATGAAAGTAATATGTATGTATGTATGTACGTATGTGTAGCTGATTGTCTAGGATAATACAAAATAAAAAAAAAAAAAAAA
5' EST: CF782772 [+]taattaatcccccccccccccgtgtgtaggaccggagcctgcagtaacaagctcttcgggagatattgactccatgaaagatgacagaggaagtcattgt
tattgccaagtgggattatacggcacaacaggaccaagaacttgatattagaaaaaatgaacgcctgtggctacttgatgactccaaaacctggtggagg
gtaagaaacactgccaacaaaactggatacgtgccatcaaactacgtggagaggaagaacagcttaaaaaaaggatctcttgttaaaaatttaaaggaca
ctctgggtcttggcaaaacaaaaagcaaaaaaaatagcactcgagatgcctcacccacaccaagcactgatgcggaatatccatctaatggcagcaatac
agatcgaatctatgatttaaacattcctgcagtagtcaaatttgcccaatgtaccaaagaaaagatgatgaatctgacactcgtggaaggcacacgaatg
gttgtttttggaaaatgcattgatggaatgtggctggttccctacaagggacatggtgggccggggtttcccccccaaacttttgtataaaaaaaaaacc
tttaaaaaaaacacaaacttcccaaaatttccccccaggtttttttttaaaacccccccagggggaaaaggggggccttttctcttttaaaaaccaaagg
gggggggcacaataatttccccccaaaagggggggcgtcttctcccacaaatttgtgtttttttcccaaaaaacat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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