XB-CLONE-4354715
Clone Name: IMAGE:7030287
|
Gene:
gtdc1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC080493 [+]
GATGCGCTCCAGCTTCCATTGCCTGTCTCCTTTTGCTGCCTGTGTTTCTGGGGTGCAGAAGGCTTTGACTAGAGAGAAATGAGCGTCCTGCTAATCGAAG
CCTTCTACGGAGGCTCTCATAAGCAGCTGATGGACCTCATAAATCAAGAGATTGAAGGCTGCGTTTTGTACACACTCCCGGCGAAGAAATGGCACTGGCG
AGCACGGACAGCAGCGCTGTACTTCATGCAAGCTATTCTGCCGGATGATAAGTACAGGGTTCTGTTTACAAGTTCAGTGCTGAACCTGGCAGAACTGGCG
GTGCTTCGACCAGACCTGGGGAAATTAAAGAAGATCCTCTACTTCCACGAGAACCAGCTGATCTATCCTGTTCAAAAAACGCAAGAGAGAGACTTTCAGT
ATGGCTATAACCAAATTCTTTCATGTTTAGTTGCAGATATTGTGGTGTTCAATTCCGCCTTCAACATGGAATCTTTTCTGACTTCTATTAAAACCTTTCT
GAAGAAGATACCCGACCATCGTCCTAAGAATCTAGAAGAAATCATTAGACCAAAATGCTGCGTCCTTTATTTTCCCATGACTTTTCCAGACATCAGTCAG
TTTCTGCCGGAACATAAACGTGTATCCCATACCCTAGCTGATAACATGTCATCTATGGATATCAGTCATTGCCCGAAGACATCACTCCAGGCAGAGAACA
TGTCTGTGGAATTCAGTCATGACCAAATCCAGTCCTCTATTGGAGAGTCATCTGCTGAACAAGAAAGAGCAACAATCTCTGAAAAAAATATTTGTGCCAG
TGGCGATCCTGTGATATTAAATGCAAGCAATACTTTGGCTGGAGATATTCGTCAGAAAAAGCCCTTGCATATTGTGTGGCCCCATAGATGGGAACACGAT
AAAGACCCAGAAACGTTCTTTAAAGTGTTGCTGAAACTGAAAGAAAAGGAGCTGACTTTTCATGTATCGGTCCTTGGAGAAACCTTCACCGACGTACCAG
ATATATTTTCTGAAGCCAGAATAACCTTGGGATCGTCTGTCTTGCACTGGGGCTATTTAGCCAGCAAAGATGACTATTTGCAAGCTCTCTGCATGGCTGA
TGTGGTCGTTTCGACGGCTAAACATGAATTCTTTGGCGTGGCGATGCTGGAAGCCGTGCACTGTGGCTGCTACCCCCTGTGCCCCAAATCCTTGGTCTAC
CCCGAAATATTCCCAGCGGTATATCTGTATTCTTCACCTGAACAGCTCTATAGAAAACTGGAGAATTTCTGCAAGAGGCCGGATGTTGTACGGAGACACC
GATTCCAGGGGGATACGGCACGCTTTTCTTGGGCAGCGCTACGTGGTGAATTCAGGTCTTTGCTCGCCGCCGAACCAAGGGAAGATCTGTGACAGATGGG
GCTAAGTCACAAAACTTGCAGCCTAAGGCAGAATTTGTAGAACTTTCCAGAGCGTGCCCATATTTACCTGATCAGAGAGAAAAAGAAAATCTGCAGAGGA
AGCCGAGCCTGGCTGCTTGTCATAGCTGACACAAAGCCATCTGCCACAAACCTGTGGCGGCTTCAAAACTCCAGTCCCTGCCACCACCCTCACTCAAATT
AAATACAGATTCTTTGAGACGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAA
AA
5' EST: CF782827 [+]taattaatcccccccccccccgatgcgctccagcttccattgcctgtctccttttgctgcctgtgtttctggggtgcagaaggctttgactagagagaaa
tgagcgtcctgctaatcgaagccttctacggaggctctcataagcagctgatggacctcataaatcaagagattgaaggctgcgttttgtacacactccc
ggcgaagaaatggcactggcgagcacggacagcagcgctgtacttcatgcaagctattctgccggatgataagtacagggttctgtttacaagttcagtg
ctgaacctggcagaactggcggtgcttcgaccagacctggggaaattaaagaagatcctctacttccacgagaaccagctgatctatcctgttcaaaaaa
cgcaagagagagactttcagtatggctataaccaaattctttcatgtttagttgcagatattgtggtgttcaattccgccttcaacatggaatcttttct
gacttctattaaaacctttctgaagaagatacccgaccatcgtcctaagaatctagaagaaatcattagaccaaaatgctgcgtcctttattttcncatg
acttttccagacatcagtcagtttctgccgggaacataaacgtgtatcccataccctagctgataacatgtcatctatggatatcagtcattgccccgaa
gacttcactccangcagagaacatgtctgtgggaattcagtcatggaccaaatccagtcctcctatggaaaagtcatctggctgaaccaaggaaagagca
accaatctctgaaaaaaaatatttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: