XB-CLONE-4354662
Clone Name: IMAGE:7026794
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782854
[+]
taattaatccccccccccccgtgcatttgctgatcgcagcacagaagagaaggagtatcctatcaaccaacaaaacacagcactttcaaagagatccact
ctgctcaggagctaaccttattgttggctatcaattcctctgcaactgaagccacacgtacacaagctcggatgctggatttatccattcaagggcatgc
ttaaccaactggattacacctcattatcatttttggtgctccagacttggggttttgcttaaaagaaattcaaacccctttatgtgtgaattattgaaaa
ttgtggtaggattgtttctttaacatttttattataatgatttcattttttgctctttttctccgagacgtgcatcaattttgggaaataataatagatt
tgcgacgggactttagggaaagcaaggggaaagatgatgaaagtgtggatacccataatttctaagagaaaggactgcacaaccgcaagaaggaatactt
tgggaactgtgggcaccaaatattgatttaaattaggaaagnatttaaaactgggtgtcacacacttggcatagcaacattatcttaatcagacgaaatt
ttccaaacataacttaaaaaacaatttctgaatatttacatacagggtaaattctcctacgatattaggggggatttagtgggttccacaataaacgtcg
acacgcaatcaatggtaacttcatttgtaagatacgagtgattctgtgttgcaattctggtgtggngagatgtcgtgtatattactcaggggagcgtacc
atgggtaatatacatagangngcgaggaccctgggtaaacatataatgaaaatacactcatgttngatgatgggnggtgagtaaaaagcgaaaaaaaacc
acaatgacgtgcgtgnaatacagagtggggttgtaaacgaactacataatttatcacaatcaaacataggaacctcatgcgnattatataatatctgctn
ntcactntctcttacctattatataatattttnnattttaaaatatatatcaagaaggagtagggtagcacgaaaaagtaagaagtgggggcgatgggaa
tataagaaaaagaggaaaaaaagaaaagggnatttagacctaccnccttaacaaggtgttataggtgtaactaatgcaagggacgagggatgagaaggga
gataagataaagacagtaaagagcgcgttgtcagaaaggagataatacgagatcctgtaattgagtgatcctaatngagtagaattgaagatggactgca
acatgcaatcataggcagacacataggaaagtatggattgtgtgtggaaggagaaaggacgntgacgtagtctgatgtgggagggggtagcggcntgagc
catagntggtcgtggaggaagggctgagaagaataggagaaagtatgtgtaaaataggagtaaaaaagtgcgggtacgacagtaggctacaacatgtagc
gtgtgcgtgtggtgnaactttnaatgcgagatgctagtggtcaaagcaccagtggagagacggacgtgtgaagatgatctcaacgtccgagcaatactta
gcaagttaaaactgcccgcatat
3' EST: CF782853
[+]ccttcatctccttgccttgcttccaaaagtccgtagaaaatcagtattattcccaaaattgatgcacaactcagataaaaagagcaaaaatgaaaacaat
aaaaaaaaaatgtaaaagaaaaaatctaccacaatttacaataaatcacacataaaggggtctgaacttcttttaagcaaaaccccaagtctggagcacc
aaaaatgataatgaggtgtaatccagttggttaagcatgcccttgaatggataaatccagcatccgagcttgtgtacgtgtggcttcagttgcagaggaa
ttgatagccaacaataaggttagctcctgagcagagtggatctctttgaaagtgctgtgttttgttggttgttaggatactccttctcttctgtgctgcg
atcagcaaatgcacggggggggggggattaattaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: